Para Lab - Quiz 1 Flashcards

1
Q

– most common specimen

A

stool sx

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2
Q

amount of stool collected (Formed)

A

2-5 g

about size of walnut or thumb

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3
Q

must be labelled with

A

Name
Age
Date and time of collection

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4
Q

how many stool specimens are collected in different days to increase chances of detecting the parasite

A

3

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5
Q

stool sx must be accompanied with

A

a lab request

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6
Q

brown color of stool due to

A

stercobilin

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7
Q

• Gross abnormalities – presence of abnormal structures such as

A

Mucus
o Pus
o Proglottids (Segment of a tapeworm)
o Blood

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8
Q

uses iodine and saline wet preparations for screening purposes

A

DFS

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9
Q

for detection of helminths (parasitic worms) & protozoans

A

DFS

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10
Q

For detection of trophozoite and cyst stages

A

DFS

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11
Q

Disadv of DFS

A

not sensitive because of very small amount of Sx used

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12
Q

can hide fecal organisms

A

Fecal debris

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13
Q

ADV of conc technique

A

-advantage: increases the chances of parasite detection because a larger amount of stool is used

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14
Q

DISADV of conc technique

A

-disadvantage: cannot preserve or detect trophozoites because they are easily destroyed

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15
Q

2 methods of conc technique

A

flotation

sedimentation

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16
Q

used for small eggs or cysts that tend to float

A

flotation

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17
Q

-principle of flotation

A

all eggs and cysts will float

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18
Q

flotation not suitable for protozoans but useful for demonstration of helminthic eggs

A

brine flotation

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19
Q

-Sp Gr of brine solution

A

= 1.20

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20
Q

measures specific gravity

A

Refractometer

21
Q

Advantages of brine

A

Inexpensive

• Applicable in field work

22
Q

Disadvantages of brine

A

Badly shrunken morphology of some eggs and cysts

Eggs of Clonorchis and Opistorchis do not float

23
Q

useful for: recovery of protozoan cysts and helminth eggs

A

ZnSO4 flotation

24
Q

sp gr in zinc sulfate flotation

A

Sp Gr = 1.18 – 1.20

25
Q

ZnSO4 flotation NOT useful for

A
  • Large trematode eggs
  • Some tapeworm (cestode) eggs
  • Ascaris lumbricoides unfertilized eggs
26
Q

useful for detection of Cryptosporidium

A

Sheather’s Sugar Flotation

27
Q

Solution added in Sheather’s Sugar Flotation

A

sucrose-phenol solution

28
Q

used for heavy or large eggs or cysts

A

sediments

29
Q

FECT meaning

A

Formalin Ether Concentration Technique

30
Q

FECT Components
Components:
– preservative/fixative

A

10% formalin

31
Q

FECT Components
removes fecal fats
-difficult and dangerous to handle, highly volatile

A

ether

32
Q

FECT Components

to strain fecal suspension

A

gauze

33
Q
FECT Components
optional only (done for better visualization
A

Lugol’s iodine

34
Q

FECT Components

removes fecal debris

A

conical tube

35
Q

a substitute for 10% formalin but may destroy Blastocystis hominis

A

distilled water

36
Q

susbtitute for ether

A

ethyl acetate

37
Q

4 Layers that will be Formed in sedimentation (from top to bottom)

A
  1. Ether
  2. Debris
  3. Formalin
  4. sediment
38
Q
  1. Used for quantitating helminth eggs
  2. Used to determine intensity of infection (↑ eggs, ↑ intensity of infection)
    - results expressed as EPG or eggs per gram
A

kato-katz technique

39
Q

another name of kato-katz

A

cellophane technique

40
Q

KK components

pleasing to the eyes; soaked overnight in glycerol

A

Green cellophane

41
Q

sub for green cellophane

A

substitute: 3% Aqueous Malachite Green or 3% Methylene Blue Stain + transparent cellophane

42
Q

KK components

acts as a clearing agent to be able to view the Sx clearer

A

50% glycerol –

43
Q

KK components

calibrated

A

template

44
Q

template thickness and diameter

A

thick: 1.5 mm
dia: 6mm

45
Q

KK components

removes large fecal debris for easy viewing

A

Wire mesh

46
Q

KK components
– used to evenly distribute/spread stool
-

A

Rubber stopper/cork

47
Q

sub for rubber stopper or cork

A

substitute: mineral bottle cover

48
Q

KK components

used in pressing the wire mesh and scraping of the sieved stool

A

Popsicle stick

49
Q

hastens the clearing process

A

Heat (40C) or direct sunlight