Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Botany > Bacte Lec - Staph > Flashcards

Bacte Lec - Staph Flashcards

1
Q

gram reaction of staph

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

color of staph in gram stain

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

gram + spherical cells which normally colonize the skin

A

micrococcus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

morphology of micrococcus

A

cubes of 8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

coagulase reaction of micrococcus

A

negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

coccus bacteria in marine environment

rarely assoc with human infection

A

planococcus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

test to diff strep and staph

A

catalase test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

catalase rxn of stapg

A

+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

all staph are [aerobe or anaerobe]

A

facultative anaerobe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

only obligate anaerobe staph

A

S. saprophyticus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

hemolytic rxn of staph

A

beta or non-hemolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

reaction of staph in catalase [describe]

A

bubble formation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

catalase test not used on blood agar bec

A

blood has coagulase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

rxn of staph on SIM

A

confined; not turbid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

common isolate on wound infection

A

suppurative type of infection [pus-forming]

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

habitant of skin and mucous membrane

A

staph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

found in environment on dust

inanimate objects

A

staph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

single most impt test to differentiate pathogenic staph from other species

A

coagulase test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

coagulase producing staph

A 
Hyicus
Aureus
Delphini
Intermedius
Schleiferi
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

all coagulase producing staph are animal species except

A

aureus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

virulence factors of staph [5]

A 
enterotoxin
exfoliative toxin
cytolytic toxin
enzymes
protein A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

type of VF

heat stable

A

enterotoxin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

type of enterotoxin that are resistant to gastric acid and digestive juices

A

enterotoxin A and enterotoxin D

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

produce acid in the form of HCl

A

parietal cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

type of VF
phage group II
involvement of skin
formation of blisters

A

Exfoliative toxin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

type of exfoliative toxin Observed among tampon

A

Toxic shock syndrome toxin 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

type of exfoliative toxin
sloughing off of epidermal layer of skin
scalded skin syndrome [Ritter disease]

A

epidermolytic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

staph infection most observed where

A

skin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

type of cytolytic toxin

platelets destroyed

A

alpha toxin/ hemolysin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

type of cytolytic toxin
injury on most cells on culture and to leukocytes
less lethal

A

delta hemolysin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

another name for leukocidin

A

Panton-Valentine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

type of cytolytic
lethal to PMN [macrophage]
invasiveness of organism

A

Leukocidin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

another name for beta hemolysin

A

hot cold lysin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

attacks sphingomyelin of RBC membrane

A

beta hemolysin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

enzymes as VF [3]

A

Coagulase
Hyaluronidase
Lipase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

type of enzyme
clumping factor
virulence marker

A

coagulase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

type of enzyme
coagulase + and coagulase -
acts on fats and oils
responsible for colonization

A

lipases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

type of enzyme
hydrolyze hyaluronic acid
with exfoliation
tries to make skin loose so bacteria can spread

A

hyaluronidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

another name for hyaluronidase

A

Durans-Reynal Factor or Spreading factor

40
Q

type of VF with phagocytic activity

A

protein A

41
Q

epidemiology of staph [3]

A

anterior nares of human carriers
hospital outbreaks [common nosocomial infections]
infant nurseries, burn units, invasive procedures

42
Q

transmission of staph [2]

A 
direct contact
community-acquired infections
poor hygiene
fomite transmission [nonliving]
carriers
43
Q

how to prevent s.aureus infection

A

hand washing

44
Q

Cutaneous infection [4]

A

bullous impetigo
boils
furuncles
carbuncles

45
Q

toxin induced diseases [4]

A

food poisoning [refrigerated foods rich with creamy sauces & mayonnaise]
toxic shock syndrome
scalded skin syndrome
toxic epidermal necrolysis

46
Q

meaning of SSS

A

Scalded Skin Syndrome

47
Q

meaning of TEN

A

toxic epidermal necrolysis

48
Q

resolution for toxic induced diseases

A

steroid therapy

49
Q

disease observed among young menstruating females

A

toxic shock syndrome

50
Q

common among sexually active young women

A

S. saprophyticus

51
Q

also seen in older men with indwelling catheter

A

S. saprophyticus

52
Q

resistant to novobiocin

A

S. saprophyticus

53
Q

most common hospital acquired UTI

A

catheter infection

54
Q

causes UTI

A

S. saprophyticus

55
Q

associated with pyelonephritis and cystitis

A

S. saprophyticus

56
Q

a multisystemic disease characterized by high fever, hypotension, shock
other conditions are pneumonia, endocarditis, osteomyelitis, septic arthritis

A

toxic shock syndrome

57
Q

isolation and identification culture media used [4]

A

thioglycollate broth
MSA
PEA
CNA

58
Q

CNA meaning

A

columbia colistin nalidixic agar

59
Q

PEA meaning

A

phenylethyl alcohol agar

60
Q

MSA meaning

A

mannitol salt agar

61
Q

cm which differentiates S. aureus from other staph

A

MSA

62
Q

cm which eliminates Gram (-) only gram + is allowed to grow

A

PEA

63
Q

colony size of aureus, epidermidis, saprophyticus

A

pinhead

64
Q

type of staph beta hemolytic

A

aureus

65
Q

type of staph non-hemolytic [2]

A

epidermidis and saprophyticus

66
Q

color of colonies of aureus

A

yellow, tan, orange

67
Q

color of colonies of epidermidis

A

white

68
Q

color of colonies of saprophyticus

A

yellow

69
Q

a clear zone indicates

A

hemolytic rxn

70
Q

substrate of catalase test

A

3% hydrogen peroxide

71
Q

genus + for catalase

A

staph

72
Q

genus - for catalase

A

strep

enterococcus

73
Q

blood used in coagulase test

A

citrated blood

74
Q

coagulase + bacteria

A

s. aureus

75
Q

coagulase - bacteria

A

epidermidis or saprophyticus

76
Q

novobiocin susceptible

A

epidermidis

77
Q

novobiocin resistant

A

saprophyticus

78
Q

coagulase test - for cell bound coagulase

A

slide test

79
Q

rgts in slide test

A

human, rabbit, dog plasma

80
Q

type of glass slide used in slide test

A

smoked glass slide

81
Q

coagulase test for free coagulase

A

tube coagulase test

82
Q

number of hours of incubation for tube coag test

A

4 hrs

83
Q

why is there a need to check every time for the clot during the whole incubation period

A

S. aureus has hyaluronidase which can liquefy the clot = false negative

84
Q

in msa, what does a yellow color mean

A

acid fermentation/LF

85
Q

in msa, what does a red color mean

A

alkaline

86
Q

what type of test rxn involves clearing of toluidine blue

A

DNAse Test Reaction

87
Q

base medium of DNAse Test Reaction

A

nutrient agar

88
Q

reagent in DNAse Test Reaction

A

dilute HCl

89
Q

in DNAse Test Reaction, it is an indication of nucleic acid hydrolysis

A

blue green to pink

90
Q

no clearing means what in DNAse Test Reaction

A

DNA not utilized

91
Q

(+) in DNAse Test Reaction means

A

Hydrolysis of DNA

92
Q

agar in gelatinase test

A

gelatin

93
Q

reaction of gelatinase producing organism in gelatinase test

A

gelatin will liquefy

94
Q

test to differentiate saprophyticus from other species

A

thermostable nuclease test/ novobiocin testing

95
Q

s. aureus enzyme stable at what temp

A

40C

96
Q

Other methods: rapid testing

A 
SEROSTAT
Slide Test
Staphylatex
Accu-staph
staphurex
staphyloslide
hemastaph
staphase

Botany (324 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions