BACTE LAB - AFS Flashcards
Slides must be
new clean free from grease free from fingerprints unscratched
what to write on the frosted end of the slide
laboratory register serial number
order number of the sputum specimen
type of pencil to use on unfrosted slides
diamond pencil, left end of the slide
what part of the sputum must be taken
yellowish purulent part
smear shape
oval
smear size
2 cm wide x 3 cm length
the smear size corresponds to how many visual fields
150, to be counted in one length
if wire loop was used instead of applicator stick, dip it in ___
alcohol washed-sand bottle
clean the wire loop in a flask containing ___ before sterilizing in a flame
70% - 90% alcohol with glass beads or sand
for accidental contamination with sputum,
hand:
wash with soap and water
for accidental contamination with sputum,
cloth:
spray with 5% phenol OR
Soak in disinfectant OR
Autoclave OR
Boil
for accidental contamination with sputum,
Surface of container,
wear rubber gloves before handling it
for accidental contamination with sputum,
bench of floor
spray with 5% phenol or Lysol
How to dry the smears
air dry, not in direct sunlight or over a flame
Elements of Quality Smear
Quality of sputum size of smear thickness of smear evenness of smear staining technique cleanness of slide
What will happen if during fixation the slide was heated for too long or kept stationary
smear will be scorched
a good stained smear by ZN method shows ___
strong red AFB, against weak blue background
It is not possible to decolorize if _____ are used
cold watery acids
To prepare 0.3% Ziehl’s carbol fuchsin working solution, mix
10 ml solution A and 90 ml solution B
Solution A of Ziehl’s carbol fuchsin
Fuchsin Alcoholic Stock Solution
Solution A of Ziehl’s carbol fuchsin components
3g basic fuchsin
95% alcohol UP to 100 mL
Solution A of Ziehl’s carbol fuchsin NAME
Aqueous Phenol Solution
Solution A of Ziehl’s carbol fuchsin components
Phenol crystals 5g
Distilled water up to 90 mL
Before adding water, liquefy the phenol crystals by
gentle heating