HEMA LAB- Methods of Blood Collection Flashcards
peripheral blood is obtained from which sites (2) in adults
1) tip of 3rd or 4th finger
2) earlobe
ideal depth of puncture in skin puncture
2-3 mm
why must squeezing puncture site in skin puncture be avoided
alters the composition of blood
difficulty in blood collection in skin puncture can be relieved by
applying warm compress
essential for tests that require anticoagualation (Except for microtechniques)
venipuncture
most convenient method of blood collection
venipuncture
reason why venipuncture is the most convenient method of blood collection
it reduces the number and variety of apparatus required
methods of performing venipuncture
syringe method
evacuated tube method
depth of needle in venipuncture
0.5 - 1 cm
after obtaining required amount of blood, patient must never ____
flex the arm
peripheral blood from infants obtained from (2)
heel
big toe
peripheral blood from newborns obtained from
heel
method recommended for whole blood clotting time technique
two-syringe method
what happens to the jugular veins when the infant cries
becomes more prominent
thin, flexible, hollow tubes inserted and positioned into a vein in order to access the bloodstream for delivery of therapies
CVADs
CVAD meaning
Central Venous Access Devices
two types of CVAD
catheter
port
type of CVAD
have one end positioned externally like PICC
catheter
PICC meaning
Peripherally inserted central catheters
type of CVAD
surgically placed internally
port
3 methods/procedure of blood collection from CVAD
Discard method
Push-pull method
Re-infusion method
angle in arterial puncture
35 - 40
prevention of hemolysis during blood collection skin puncture (2)
use sharp lancet
avoid squeezing area near the site
prevention of hemolysis during blood collection
venipuncture (7)
- use a sharp, smooth needle
- penetrate uppermost wall of the vein fully
- check and make sure the tourniquet is NOT TOO TIGHT when aspirating blood
- aspirate blood gently
- remove needle before transferring blood to tubes (syringe method)
- mix tube by gentle inversion if the tubes contain additives
- if serum is needed, do not rim and centrifuge the blood until a clot has formed
prevention of hemolysis during blood collection arterial puncture (2)
use a sharp needle
avoid excessive needle manipulation
how many mL of blood is aspirated when using the discard method of blood collection from CVAD
3-6 mL
how many mL of blood is aspirated when using the re-infusion method of blood collection from CVAD
3-6 mL
how many mL of blood is aspirated when using the push-pull method of blood collection from CVAD
6 mL
CVAD is flushed with what solution
normal saline solution
CVAD is flushed with NSS in which method/s
push-pull
discard
vein that runs from an angle of the mandible to the submandibular area
external jugular vein
how to immobilize arms of the infant during external jugular vein puncture
wrap infant in a sheet
