CC LAB - TPAG

Question 1

􏰀 Based on measurement of nitrogen content 􏰀

Answer 1

Kjeldahl Method

Question 2

Reference method

Answer 2

Kjeldahl Method

Question 3

􏰀 Digesting agent of Kjeldahl Method

Answer 3

H2SO4

Question 4

Most commonly used

􏰀

Answer 4

Biuret Method

Question 5

Need two peptide bonds and an alkaline medium

Answer 5

Biuret Method

Question 6

􏰀 Composition of Biuret Reagent:

Answer 6

a) Copper sulfate- provides the cupric ion that complexes with the peptide bonds
b) Potassium sodium tartrate (Rochelle’s salt)- prevents precipitation in alk solution
c) Potassium iodide- prevents autoreduction of copper

Question 7

• important in maintaining plasma oncotic pressure

Answer 7

Albumin

Question 8

family of globular proteins that have higher molecular weights than albumin and are insoluble in pure water

Answer 8

Globulin-

Question 9

Inverted A:G ratio means

Answer 9

presence of myeloma

Question 10

Immediate response occurs with stress or inflammation caused by infection, injury or surgical trauma

Answer 10

Acute inflammatory response

Question 11

• normal or ↓ albumin

* ↑ α1 and α2 globulins

Answer 11

Acute inflammatory response

Question 12

Late response is correlated with chronic infection

autoimmune diseases, chronic liver disease, chronic infection, cancer

Answer 12

Chronic inflammatory response

Question 13

• normal or ↓ albumin
• ↑α1 or α2 globulins
• ↑↑ γ globulins

Answer 13

Chronic inflammatory response

Question 14

Cirrhosis can be caused by [2]

Answer 14

chronic alcohol abuse or viral hepatitis

Question 15

↓ albumin
• ↓ α1, α2 and β globulins
• ↑ Ig A in γ-fraction

Answer 15

Liver damage - Cirrhosis

Question 16

the kidney damage illustrates the long term loss of lower molecular weight proteins

Answer 16

Nephrotic syndrome

Question 17

retention of higher molecular weight proteins (↑↑ α2-macroglobulin and ↑β-globulin)

Answer 17

Nephrotic syndrome

Question 18

↓ albumin and IgG – they are filtered in kidney)

Answer 18

Nephrotic syndrome