GPHT LAB - Histopathologic Techniques

Question 1

process of indicating the number of specimen by means of a pencil, in order to properly identify the specimen
properly speaking is the first step in all histopathologic techniques

Answer 1

numbering

Question 2

is the first step in all histopathologic techniques

Answer 2

properly speaking

Question 3

process of preserving cells and tissue constituents in a condition identical to that existing during life and to do this is a way that will allow preparation of thin stained sections

Answer 3

fixation

Question 4

process of water removal from the tissue prior to replacement by wax. water is immiscible with paraffin or wax

Answer 4

dehydration

Question 5

process whereby the alcohol in the tissues is replaced by afluid that will dissolve the wax with which the tissue must be impregnated

Answer 5

clearing or dealcoholization

Question 6

process that involves the impregnation of tissue with a medium that will fill the natural cavities, spaces, and interstices of the tissues

Answer 6

wax or paraffin impregnation

Question 7

process that involves the use of molds for the purpose of setting the embedding medium to a sufficient consistency in order to allow the cutting of suitably thin sections w/o undue distortion, w/o alteration of spatial relationships of the tissue and cellular elements

Answer 7

embedding

Question 8

facilitates sectioning

Answer 8

embedding

Question 9

process that involves separation of one tissue block from another using sharp knife (performed only when one uses a compound embedding unit)

Answer 9

blocking

Question 10

process that involves the cutting of excess wax (in thin slices to prevent the block from cracking) from the tissue block so that the block forms a four-sided prism or truncated pyramid opposite sides being parallel (this is most important if serial sections are desired)

Answer 10

trimming

Question 11

process of cutting very thin slices of tissues accomplished with a microtome

Answer 11

sectioning

Question 12

apparatus for sectioning

Answer 12

microtome

Question 13

process w/c involves the use of dyes or stains for optically differentiating the cellular and tissue constituents and to determine the free chemical nature of some details in the cells (histochemical staining)

Answer 13

staining

Question 14

process w/c involves the use of a medium and cover slip to facilitate the ease of handling and storage of slide and to prevent damage to the section

Answer 14

mounting

Question 15

process of indicating the year and the specimen number on one end of the prepared slide for proper identification

Answer 15

labeling

Question 16

used to indicate the number of the specimen

Answer 16

pencil

Question 17

first step in all histopathologic techniques

Answer 17

properly speaking

Question 18

allows for the preparation of thin stained sections

Answer 18

fixation

Question 19

why is dehydration performed

Answer 19

water is immiscible with paraffin or wax

therefore water must be removed before impregnating the tissue with wax

Question 20

purpose of embedding (2)

Answer 20

allow the cutting of thin sections without undue distortion and without alteration of spatial relationships of tissue and cellular elements
facilitates sectioning

Question 21

instrument used in blocking

Answer 21

sharp knife

Question 22

performed only when one uses a compound embedding unit

Answer 22

blocking

Question 23

purpose of staining (2)

Answer 23

to optically differentiate the cellular and tissue constituents
to determine the free chemical nature of some details in the cells (histochemical staining)

Question 24

purposes of mounting (2)

Answer 24

facilitate the ease of handling and storage of slide

to prevent damage to the section

Question 25

purpose of labeling

Answer 25

proper identification

Question 26

materials used in mounting

Answer 26

medium and cover slip

Question 27

another name for dealcoholization

Answer 27

clearing

Question 28

in dealcoholization, alcohol is replaced by what?

Answer 28

a fluid that will dissolve the wax with which the tissue must be impregnated

Question 29

why use molds in embedding?

Answer 29

to set the embedding medium to a sufficient consistency

Question 30

why set the medium to a sufficient consistency

Answer 30

to allow the cutting of suitably thin sections w/o undue distortion and w/o alteration of the spatial relationships of tissue and cellular elements

Question 31

why must slices be thin when trimming

Answer 31

to prevent the block from cracking

Question 32

shape of block in trimming (2)

Answer 32

four-sided prism or truncated pyramid opposite sides being parallel

Question 33

this is most important if serial sections are desired

Answer 33

opposite sides of the block are parallel

Question 34

determining the free chemical nature of some details in cells

Answer 34

histochemical staining

Question 35

what must be indicated when labeling

Answer 35

specimen number and year

Question 36

blocking is only performed when one uses a

Answer 36

compound embedding unit