GPHT LAB - Histopathologic Techniques Flashcards
process of indicating the number of specimen by means of a pencil, in order to properly identify the specimen
properly speaking is the first step in all histopathologic techniques
numbering
is the first step in all histopathologic techniques
properly speaking
process of preserving cells and tissue constituents in a condition identical to that existing during life and to do this is a way that will allow preparation of thin stained sections
fixation
process of water removal from the tissue prior to replacement by wax. water is immiscible with paraffin or wax
dehydration
process whereby the alcohol in the tissues is replaced by afluid that will dissolve the wax with which the tissue must be impregnated
clearing or dealcoholization
process that involves the impregnation of tissue with a medium that will fill the natural cavities, spaces, and interstices of the tissues
wax or paraffin impregnation
process that involves the use of molds for the purpose of setting the embedding medium to a sufficient consistency in order to allow the cutting of suitably thin sections w/o undue distortion, w/o alteration of spatial relationships of the tissue and cellular elements
embedding
facilitates sectioning
embedding
process that involves separation of one tissue block from another using sharp knife (performed only when one uses a compound embedding unit)
blocking
process that involves the cutting of excess wax (in thin slices to prevent the block from cracking) from the tissue block so that the block forms a four-sided prism or truncated pyramid opposite sides being parallel (this is most important if serial sections are desired)
trimming
process of cutting very thin slices of tissues accomplished with a microtome
sectioning
apparatus for sectioning
microtome
process w/c involves the use of dyes or stains for optically differentiating the cellular and tissue constituents and to determine the free chemical nature of some details in the cells (histochemical staining)
staining
process w/c involves the use of a medium and cover slip to facilitate the ease of handling and storage of slide and to prevent damage to the section
mounting
process of indicating the year and the specimen number on one end of the prepared slide for proper identification
labeling
used to indicate the number of the specimen
pencil
first step in all histopathologic techniques
properly speaking
allows for the preparation of thin stained sections
fixation
why is dehydration performed
water is immiscible with paraffin or wax
therefore water must be removed before impregnating the tissue with wax
purpose of embedding (2)
allow the cutting of thin sections without undue distortion and without alteration of spatial relationships of tissue and cellular elements
facilitates sectioning
instrument used in blocking
sharp knife
performed only when one uses a compound embedding unit
blocking
purpose of staining (2)
to optically differentiate the cellular and tissue constituents
to determine the free chemical nature of some details in the cells (histochemical staining)
purposes of mounting (2)
facilitate the ease of handling and storage of slide
to prevent damage to the section
purpose of labeling
proper identification
materials used in mounting
medium and cover slip
another name for dealcoholization
clearing
in dealcoholization, alcohol is replaced by what?
a fluid that will dissolve the wax with which the tissue must be impregnated
why use molds in embedding?
to set the embedding medium to a sufficient consistency
why set the medium to a sufficient consistency
to allow the cutting of suitably thin sections w/o undue distortion and w/o alteration of the spatial relationships of tissue and cellular elements
why must slices be thin when trimming
to prevent the block from cracking
shape of block in trimming (2)
four-sided prism or truncated pyramid opposite sides being parallel
this is most important if serial sections are desired
opposite sides of the block are parallel
determining the free chemical nature of some details in cells
histochemical staining
what must be indicated when labeling
specimen number and year
blocking is only performed when one uses a
compound embedding unit
