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Botany > HEMA 2 LEC - Cytochemistry > Flashcards

HEMA 2 LEC - Cytochemistry Flashcards

1
Q

In vitro staining of cells that allows microscopic examination of the cell’s chemical composition

A

Cytochemistry

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2
Q

Differentiate cell lineage of blasts in acute leukemia

A

Cytochemistry

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3
Q

are used if constituents are sensitive to heat, light, storage, and processing techniques

A

Fresh smears

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4
Q

can be used for stable constituents

A

Aged specimens

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5
Q

Types of Cytochemical Staining

A

Enzymatic

NonEnzymatic

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6
Q

Stains alkaline phosphatase present in the neutrophil

A

Leukocyte Alkaline Phosphatase Stain

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7
Q

Used to differentiate
Chronic myelogenous leukemia from leukemoid reaction
Polycythemia vera from secondary erythrocytosis

A

Leukocyte Alkaline Phosphatase Stain

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8
Q

Normal range LAP

A

30-185

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9
Q

Stains peroxidase in granules of neutrophils, eosinophils, and monocytes

A

Myeloperoxidase Stain

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10
Q

Used to differentiate AML and AMML from ALL (2)

A

Myeloperoxidase Stain

Sudan Black B Stain

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11
Q

Show similar results with Sudan Black B stain

A

Myeloperoxidase Stain WHEN Used to differentiate AML and AMML from ALL

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12
Q

Peroxidase activity is ___ in Mature neutrophils in CML

A

inc

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13
Q

Peroxidase activity is ___ in Infection, AML, myelodysplastic conditions

A

decreased

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14
Q

Differentiate acute leukemias that are myeloid (M1, M2. M3) from monocytic origin (M5)

A

Esterases

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15
Q

Stains mucoproteins, glycoproteins, and HMW carbohydrates in all cells except pronormoblast

A

Periodic Acid-Schiff

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16
Q

Used for the diagnosis of ALL, some subtypes of AML, and DiGuglielmo’s disease

A

Periodic Acid-Schiff

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17
Q

PAS Reactions

Granulocytes

A

Intensity of stain increases as cells mature

18
Q

PAS Reactions

Megakaryocytes

A

Finely diffuse stain

19
Q

PAS Reactions

Platelets

A

Intensely red pink

20
Q

PAS Reactions
M6
Early erythroblast –

A

course and granular

21
Q

PAS Reactions

M6 Late erythroblast –

A

diffusely positive

22
Q

Lipids like sterols, phospholipids, neutral fats in monocytes and granulocytes

A

Sudan Black B Stain

23
Q

WHAT STAIN

Hairy cell leukemia

A

Acid phosphatase stain (with tartrate resistance

24
Q

WHAT STAIN

Differentiate granulocytic from monocytic leukemia

A

Nonspecific esterase stain (with fluoride inhibition)

25
Q

WHAT STAIN

Esterases in granulocytic cells

A

Chloroacetate esterase stain

26
Q

WHAT STAIN

Screening procedure for metabolic defects in neutrophil

A

Nitroblue tetrazolium (NBT)

27
Q

Dec LAP Activity

A 
CML - Chronic Myelogenous Leukemia
Sickle Cell Anemia
Sideroblastic Leukemia
Paroxysmal Nocturnal Hemoglobinuria
Marked eosinophilia
Infectious Mononucleosis
28
Q

Inc LAP Activity (LIP PC MOHAM)

A 
Leukemoid reaction
Infection
pregnancy (last trimester)
polycythemia vera
corticosteroid therapy
myelosclerosis
obstructive jaundice
hodgkin's disease
aplastic leukemia
multiple myeloma
29
Q

Normal LAP activity (LUVS)

A

Untreated hemolytic anemia
Lymphosarcoma
Viral hepatitis
Secondary erythrocytosis

30
Q

LAP Score

0

A

No staining

31
Q

LAP Score

1+

A

faint and diffuse staining

32
Q

LAP Score

2+

A

pale with moderate blue staining

33
Q

LAP Score

3+

A

strong blue precipitated staining

34
Q

LAP Score

4+

A

deep blue or brilliant staining with no visible cytoplasm

35
Q

Myeloperoxidase Stain stains granules in which cells

A

neutrophils, eosinophils and monocytes

36
Q

appearance of basophil and lymphocyte with myeloperoxidase stain

A

unstained

37
Q

Periodic Acid-Schiff Stains _____ (3) in all cells except pronormoblast

A

mucoproteins, glycoproteins, and HMW carbohydrates

38
Q

PAS used in the dx of (3)

A

ALL, some subtypes of AML, and DiGuglielmo’s disease

39
Q

PAS Rxns of lymphocytes in all

A

vary staining pattern, may be coarse or fine diffuse

40
Q

PAS rxns of L3 or Burkitt

A

PAS negative

41
Q

PAS negative

A

L3 or Burkitt

42
Q

Sudan Black B stain stains Lipids like sterols, phospholipids, neutral fats in what cells (2)

A

monocytes and granulocytes

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