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Botany > GPHT LAB - Apparatus I > Flashcards

GPHT LAB - Apparatus I Flashcards

1
Q

machine on which thin sections are cut

A

microtome

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2
Q

process of using a microtome

A

microtomy

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3
Q

principle of microtomy

A

pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness

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4
Q

3 essential parts of a microtome

A

1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl

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5
Q

An essential part of the microtome used for actual cutting of tissue sections

A

knife and knife carrier

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6
Q

An essential part of the microtome where the tissue is held in position

A

block holder or chuck

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7
Q

An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections

A

ratchet feed wheel, adjustment screws, pawl

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8
Q

An essential part of the microtome adjusted or manipulated during microtomy

A

ratchet feed wheel, adjustment screws, pawl

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9
Q

types of microtome (5)

A 
rotary (minot)
rocking 
freezing
ultra-thin
sliding or base sledge
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10
Q

microtome invented by Caldwell Trefall

A

Rocking

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11
Q

microtome invented by Queckett

A

Freezing

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12
Q

microtome invented by Minot

A

Rotary (Minot)

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13
Q

microtome invented in 1881

A

Rocking

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14
Q

microtome invented in 1885 - 1886

A

Rotary (Minot)

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15
Q

microtome invented in 1848

A

freezing

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16
Q

microtome developed by Adams

A

Sliding or Base Sledge

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17
Q

microtome developed in 1789

A

Sliding or Base Sledge

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18
Q

microtome also known as Cambridge

A

Rocking

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19
Q

Rocking Microtome also known as

A

Cambridge

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20
Q

simplest of all microtomes

A

Rocking

21
Q

what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block

A

Rocking

22
Q

disadvantage of rocking microtome

A

will not produce GOOD serial sections

23
Q

ribbons of how many sections are produced w/ a rocking microtome

A

60-90

24
Q

thickness of the sections produced by a rocking micrometer in um

A

10-12 um

25
Q

what type of microtome

relatively small length of knife is available for use; dangerously placed w/ the blade up

A

rotary (minot)

26
Q

what type of microtome

not suitable for cutting large blocks

A

rotary (minot)

27
Q

what type of microtome

for cutting serial sections

A

rotary (minot)

28
Q

what type of microtome

most commonly used; present in the lab

A

rotary (minot)

29
Q

what type of microtome

able to section paraffin-embedded sections

A

rotary (minot)

30
Q

what type of microtome
consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder

A

freezing

31
Q

in the freezing microtome, what allows the release of rapid intermittent burst of carbon dioxide

A

simple lever-operated valve

32
Q

in the freezing microtome, what is the propellant or freezing agent

A

carbon dioxide

33
Q

what type of microtome

used for rush frozen sections

A

freezing

34
Q

what type of microtome

capable of cutting sections at 0.5 micron required for EM

A

ultra-thin

35
Q

what type of microtome

knife contains selected fragments of broken plate glass

A

ultra-thin

36
Q

what knife can be used for ultra-thin microtome

A

special diamond knife

37
Q

what type of microtome

for cutting extremely hard blocks and for frozen sections

A

sliding or base sledge

38
Q

what type of microtome

first microtome invented

A

sliding or base sledge

39
Q

microtome knife is made of what?

A

stainless steel

40
Q

histology technician’s too of his profession

A

microtome knife

41
Q

personal possession of histo tech

A

microtome knife

42
Q

every technician should be given at least __ good knives

A

2

43
Q

3 basic shapes of microtome knife

A

biconcave
plane concave
plane wedge

44
Q

length of biconcave

A

120 mm

45
Q

microtome knife used for paraffin sections with rocking or rotary type microtome

A

biconcave

46
Q

microtome knife that can be used on a carbon dioxide freezing microtome

A

biconcave

47
Q

microtome knife very useful for cutting paraffin tissue blocks

A

biconcave

48
Q

biconcave knife is found on what microtome

A

rotary

Botany (324 decks)

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