GPHT LAB - Apparatus I Flashcards
machine on which thin sections are cut
microtome
process of using a microtome
microtomy
principle of microtomy
pawl is brought into contact with a ratchet wheel, which is connected to a mill head micrometer screw. this action turns the ratchet wheel, which in turn rotates the screw. By this means the block is moved towards the knife at a predetermined thickness
3 essential parts of a microtome
1) knife and knife carrier
2) block holder or chuck
3) ratchet feed wheel, adjustment screws, pawl
An essential part of the microtome used for actual cutting of tissue sections
knife and knife carrier
An essential part of the microtome where the tissue is held in position
block holder or chuck
An essential part of the microtome used to line up the tissue block in proper position with the knife, adjust the proper thickness of the tissue for successive sections
ratchet feed wheel, adjustment screws, pawl
An essential part of the microtome adjusted or manipulated during microtomy
ratchet feed wheel, adjustment screws, pawl
types of microtome (5)
rotary (minot) rocking freezing ultra-thin sliding or base sledge
microtome invented by Caldwell Trefall
Rocking
microtome invented by Queckett
Freezing
microtome invented by Minot
Rotary (Minot)
microtome invented in 1881
Rocking
microtome invented in 1885 - 1886
Rotary (Minot)
microtome invented in 1848
freezing
microtome developed by Adams
Sliding or Base Sledge
microtome developed in 1789
Sliding or Base Sledge
microtome also known as Cambridge
Rocking
Rocking Microtome also known as
Cambridge
simplest of all microtomes
Rocking
what type of microtome in which the tissue block being mounted on the end of a spring poised rocking arm, the knife held in horizontal position, blade face up and slightly inclined towards the block
Rocking
disadvantage of rocking microtome
will not produce GOOD serial sections
ribbons of how many sections are produced w/ a rocking microtome
60-90
thickness of the sections produced by a rocking micrometer in um
10-12 um
what type of microtome
relatively small length of knife is available for use; dangerously placed w/ the blade up
rotary (minot)
what type of microtome
not suitable for cutting large blocks
rotary (minot)
what type of microtome
for cutting serial sections
rotary (minot)
what type of microtome
most commonly used; present in the lab
rotary (minot)
what type of microtome
able to section paraffin-embedded sections
rotary (minot)
what type of microtome
consists of a central screw by which the block holder is perforated, attached to it is the food pipe carrying carbon dioxide from a cylinder
freezing
in the freezing microtome, what allows the release of rapid intermittent burst of carbon dioxide
simple lever-operated valve
in the freezing microtome, what is the propellant or freezing agent
carbon dioxide
what type of microtome
used for rush frozen sections
freezing
what type of microtome
capable of cutting sections at 0.5 micron required for EM
ultra-thin
what type of microtome
knife contains selected fragments of broken plate glass
ultra-thin
what knife can be used for ultra-thin microtome
special diamond knife
what type of microtome
for cutting extremely hard blocks and for frozen sections
sliding or base sledge
what type of microtome
first microtome invented
sliding or base sledge
microtome knife is made of what?
stainless steel
histology technician’s too of his profession
microtome knife
personal possession of histo tech
microtome knife
every technician should be given at least __ good knives
2
3 basic shapes of microtome knife
biconcave
plane concave
plane wedge
length of biconcave
120 mm
microtome knife used for paraffin sections with rocking or rotary type microtome
biconcave
microtome knife that can be used on a carbon dioxide freezing microtome
biconcave
microtome knife very useful for cutting paraffin tissue blocks
biconcave
biconcave knife is found on what microtome
rotary
