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Botany > GPHT LAB - Infiltration > Flashcards

GPHT LAB - Infiltration Flashcards

0
Q

why impregnate

A

to give tx a firm consistency

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1
Q

filling up of empty spaces cavities using an impregnating medium

A

infiltration

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2
Q

best and most common infiltrating medium

A

paraffin wax

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3
Q

MP of pure PW

A

56C

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4
Q

__ not be overheated

A

PW

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5
Q

PW requires __ hrs of infiltration

A

.

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6
Q

PW must be __ prior to use

A

filtered

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7
Q

coarse filter for PW

A

Green’s # 904

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8
Q

PW requires what equipment

A

paraffin oven

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9
Q

if PW is reused, heat at __ to remove water

A

100C

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10
Q

PW can be used how many times

A

2x

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11
Q

disadv of PW

A

not for fatty tissues

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12
Q

PW Substitute

paraffin + polymer

A

paraplast

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13
Q

MP of paraplast

A

56-57C

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14
Q

Quite similar to paraplast but MP is 56-58C

A

Embeddol

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15
Q

with a rubber what PW Substitute

A

tissue mat

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16
Q

harder than ordinary PW but MP = 46-48C

A

Esterwax

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17
Q

PW Substitute

Soluble in 95% EtOH

A

Esterwax

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18
Q

MP of Esterwax

A

46-48C

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19
Q

Ex. of water-soluble waxes

A

carbowax

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20
Q

Adv of carbowax

A

no need for dehydration and clearing

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21
Q

disadv of carbowax

A

expensive, tissue sections dissolved during flotation

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22
Q

microtome for carbowax

A

base sledge or sliding

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23
Q

Methods of Paraffin impregnation

Changes of PW, transfer tissue from one container to another

A

manual processing

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24
Q

Methods of Paraffin impregnation

performs steps 2-5

A

automatic tissue processing

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25
Q

Methods of Paraffin impregnation

fastest, performed at negative atmospheric pressure

A

vacuum method

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26
Q

celloidin also called

A

colloidion

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27
Q

wax for hollow large tx tend to collapse

A

celloidin

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28
Q

no heating required

A

celloidin

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29
Q

time for processing when using celloidin

A

days to weeks

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30
Q

2 methods for celloidin

for brain and bone tx

A

wet celloidin method

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31
Q

2 methods for celloidin

eyes

A

drycelloidin method

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32
Q

for enzyme studies, heat sensitive substances

A

gelatin

33
Q

no dehydration and clearing required (2)

A

gelatin

carbowax

34
Q

preservative of gelatin (to prevent mold growth)

A

1% phenol

35
Q

hard medium: can cut very thick sections

A

plastic/resins

36
Q

for preparing ultra-thin and EM sections

A

plastic/resins

37
Q

widest application, for light and EM

A

Epoxy resins

38
Q

why impregnate

A

to give tx a firm consistency

39
Q

best and most common infiltrating medium

A

paraffin wax

40
Q

MP of pure PW

A

56C

41
Q

__ not be overheated

A

PW

42
Q

PW requires __ hrs of infiltration

A

Less than 24 hours

43
Q

PW must be __ prior to use

A

filtered

44
Q

coarse filter for PW

A

Green’s # 904

45
Q

PW requires what equipment

A

paraffin oven

46
Q

if PW is reused, heat at __ to remove water

A

100C

47
Q

PW can be used how many times

A

2x

48
Q

disadv of PW

A

not for fatty tissues

49
Q

PW Substitute

paraffin + polymer

A

paraplast

50
Q

MP of paraplast

A

56-57C

51
Q

Quite similar to paraplast but MP is 56-58C

A

Embeddol

52
Q

with a rubber what PW Substitute

A

tissue mat

53
Q

harder than ordinary PW but MP = 46-48C

A

Esterwax

54
Q

PW Substitute

Soluble in 95% EtOH

A

Esterwax

55
Q

MP of Esterwax

A

46-48C

56
Q

Ex. of water-soluble waxes

A

carbowax

57
Q

Adv of carbowax

A

no need for dehydration and clearing

58
Q

disadv of carbowax

A

expensive, tissue sections dissolved during flotation

59
Q

microtome for carbowax

A

base sledge or sliding

60
Q

Methods of Paraffin impregnation

Changes of PW, transfer tissue from one container to another

A

manual processing

61
Q

Methods of Paraffin impregnation

performs steps 2-5

A

automatic tissue processing

62
Q

Methods of Paraffin impregnation

fastest, performed at negative atmospheric pressure

A

vacuum method

63
Q

celloidin also called

A

colloidion

64
Q

wax for hollow large tx tend to collapse

A

celloidin

65
Q

no heating required

A

celloidin

66
Q

time for processing when using celloidin

A

days to weeks

67
Q

2 methods for celloidin

for brain and bone tx

A

wet celloidin method

68
Q

2 methods for celloidin

eyes

A

drycelloidin method

69
Q

for enzyme studies, heat sensitive substances

A

gelatin

70
Q

no dehydration and clearing required (2)

A

gelatin

carbowax

71
Q

preservative of gelatin (to prevent mold growth)

A

1% phenol

72
Q

hard medium: can cut very thick sections

A

plastic/resins

73
Q

for preparing ultra-thin and EM sections

A

plastic/resins

74
Q

widest application, for light and EM

A

Epoxy resins

75
Q

Rarely used infiltrating medium

A

Gelatin

76
Q

Why use preservative for gelatin

A

To prevent growth of molds

77
Q

Does not require dehydration and clearing (2)

A

Carbowax

Gelatin

78
Q

Components of plastic/resins

A

Acrylic
Polyester
Glycerol
Epoxy resins

79
Q

Component of plastic/resin infiltrating medium with widest application, for LM and EM

A

Epoxy resin

Botany (324 decks)

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