GPHT LAB - Infiltration Flashcards
why impregnate
to give tx a firm consistency
filling up of empty spaces cavities using an impregnating medium
infiltration
best and most common infiltrating medium
paraffin wax
MP of pure PW
56C
__ not be overheated
PW
PW requires __ hrs of infiltration
.
PW must be __ prior to use
filtered
coarse filter for PW
Green’s # 904
PW requires what equipment
paraffin oven
if PW is reused, heat at __ to remove water
100C
PW can be used how many times
2x
disadv of PW
not for fatty tissues
PW Substitute
paraffin + polymer
paraplast
MP of paraplast
56-57C
Quite similar to paraplast but MP is 56-58C
Embeddol
with a rubber what PW Substitute
tissue mat
harder than ordinary PW but MP = 46-48C
Esterwax
PW Substitute
Soluble in 95% EtOH
Esterwax
MP of Esterwax
46-48C
Ex. of water-soluble waxes
carbowax
Adv of carbowax
no need for dehydration and clearing
disadv of carbowax
expensive, tissue sections dissolved during flotation
microtome for carbowax
base sledge or sliding
Methods of Paraffin impregnation
Changes of PW, transfer tissue from one container to another
manual processing
Methods of Paraffin impregnation
performs steps 2-5
automatic tissue processing
Methods of Paraffin impregnation
fastest, performed at negative atmospheric pressure
vacuum method
celloidin also called
colloidion
wax for hollow large tx tend to collapse
celloidin
no heating required
celloidin
time for processing when using celloidin
days to weeks
2 methods for celloidin
for brain and bone tx
wet celloidin method
2 methods for celloidin
eyes
drycelloidin method
for enzyme studies, heat sensitive substances
gelatin
no dehydration and clearing required (2)
gelatin
carbowax
preservative of gelatin (to prevent mold growth)
1% phenol
hard medium: can cut very thick sections
plastic/resins
for preparing ultra-thin and EM sections
plastic/resins
widest application, for light and EM
Epoxy resins
why impregnate
to give tx a firm consistency
best and most common infiltrating medium
paraffin wax
MP of pure PW
56C
__ not be overheated
PW
PW requires __ hrs of infiltration
Less than 24 hours
PW must be __ prior to use
filtered
coarse filter for PW
Green’s # 904
PW requires what equipment
paraffin oven
if PW is reused, heat at __ to remove water
100C
PW can be used how many times
2x
disadv of PW
not for fatty tissues
PW Substitute
paraffin + polymer
paraplast
MP of paraplast
56-57C
Quite similar to paraplast but MP is 56-58C
Embeddol
with a rubber what PW Substitute
tissue mat
harder than ordinary PW but MP = 46-48C
Esterwax
PW Substitute
Soluble in 95% EtOH
Esterwax
MP of Esterwax
46-48C
Ex. of water-soluble waxes
carbowax
Adv of carbowax
no need for dehydration and clearing
disadv of carbowax
expensive, tissue sections dissolved during flotation
microtome for carbowax
base sledge or sliding
Methods of Paraffin impregnation
Changes of PW, transfer tissue from one container to another
manual processing
Methods of Paraffin impregnation
performs steps 2-5
automatic tissue processing
Methods of Paraffin impregnation
fastest, performed at negative atmospheric pressure
vacuum method
celloidin also called
colloidion
wax for hollow large tx tend to collapse
celloidin
no heating required
celloidin
time for processing when using celloidin
days to weeks
2 methods for celloidin
for brain and bone tx
wet celloidin method
2 methods for celloidin
eyes
drycelloidin method
for enzyme studies, heat sensitive substances
gelatin
no dehydration and clearing required (2)
gelatin
carbowax
preservative of gelatin (to prevent mold growth)
1% phenol
hard medium: can cut very thick sections
plastic/resins
for preparing ultra-thin and EM sections
plastic/resins
widest application, for light and EM
Epoxy resins
Rarely used infiltrating medium
Gelatin
Why use preservative for gelatin
To prevent growth of molds
Does not require dehydration and clearing (2)
Carbowax
Gelatin
Components of plastic/resins
Acrylic
Polyester
Glycerol
Epoxy resins
Component of plastic/resin infiltrating medium with widest application, for LM and EM
Epoxy resin
