Bacte Lec - Quiz 2 Flashcards
- media supplemented with serum or sugar to prevent osmotic pressure
- capable of reverting to normal form (Residual) peptidoglycan
L-forms
– physical mordant in AF stain
Steam or heat
chemical mordant present in carbolfuchsin
Phenol –
2 Methods of Acid- Fast Staining
- Ziehl-Neelsen method– hot, steaming for 5 min
2. Kinyoun method – cold, more than 60 min
may be a substitute for methylene blue as counterstain in AF Staining
Malachite green
– placed on top of the smear before flooding with carbolfuchsin
Filter paper
function of filter paper
-prevents rapid evaporation of dye (dye is not allowed to dry)
cells commonly found in clinical specimens because it is the first to react during a bacterial infection
Neutrophils –
– round, spherical, shaped like berries or grapes
`
coccus
identify species
(flattened on one side like a coffee bean)
Neisseria gonorrhoea
multiple planes and form grapelike clusters or broad sheets
staphylococci
2 planes remain in groups of 4 (ex. Micrococcus tetragena)
• tetrads:
3 planes and remain attached in cubelike groups of 8
• sarcinae
example of species with sarcinae arrangement
Sarcina lutea
remain in pairs after dividing
diplococci:
example of species with diplococci arrangement
(Streptococcus pneumonia/Diplococcus pneumonia)
type of cocci arrangement
divide and remain in chain like pattern
streptococci:
-usually round but can be oval, elongated, flattened on one side
cocci
example of tetrad
Micrococcus tetragena
example of streptococci
streptococcus pyogenes
Arrangements of bacilli
singly, chains, pairs
Gram-positive long bacilli, a common cause of bacterial contamination in laboratories
Bacillus subtilis
arrangement of bacillus
may align themselves side by side
palisading
example of palisading
Corynebacteria or Corynebacterium diphtheria
example of staphylococcus other than staphylococcus aureus
Staphylococcus hominis
type of shape
oval and look so much like cocci
coccobacilli
example of coccobacilli
Acinetobacter spp.
Acinetobacter baumannii
Bordetella pertussis
example of vibrio
Vibrio cholerae
Example of spirilla
Leptospira interrogans
Helicobacter pylori
Example of spirochetes
Treponema pallidum
shape and arrangement of bacteria is determined by
heredity
genetically, most bacteria are (number of shapes)
monomorphic
what can alter the shape of bacteria
number of environmental conditions
bacteria is said to be ____ when it has many shapes
pleomorphic
example of pleomorphic
Rhizobium and Corynebacterium
bacteria which look like Chinese characters
Rhizobium and Corynebacterium
size of bacteria may be measured using a_______
micrometer
length of bacteria
2 to 8 um
diameter of bacteria
0.2 to 2 um
look like curved rods.
vibrios
have a helical shape and fairly rigid bodies
spirilla
have a helical shape and flexible bodies
spirochetes
a genus of halophilic archaea, are rectangular or square and flat
Haloarcula
star-shaped bacteria
Stella