GPHT LAB - Apparatus III Flashcards
apparatus used in the embedding process
tissue embedding center
apparatus w/c dispenses paraffin into mold; with separate area for cooling
tissue embedding center
parts of tissue embedding center
provides instant cooling of exchange plate
freon refrigeration system
parts of tissue embedding center
forms a pent-house on the right-hand side of the unit and on the lower left of this chamber is a microswitch dispenser
paraffin melting chamber
parts of tissue embedding center
maintains an optimal paraffin temp with adjustable thermostatic control
paraffin melting chamber
parts of tissue embedding center
filters particles or sediments
microscreen
parts of tissue embedding center
provides non-clogging flow of molten paraffin for casting of molds when plate is gently pressed
micro-switch dispenser
parts of tissue embedding center
for diff operations of embedding procedure
hot and cold orientation platforms
parts of tissue embedding center
catches excess paraffin
waste drawer
parts of tissue embedding center
to pre-heat forceps
hot well
keeps paraffin wax in melted state
paraffin oven
working temp of parafifn oven
2-5C higher than MP of wax
MP of pure paraffin wax
56C
apparatus used during fishing out
flotation water bath
color of flotation water bath
black
why the flotation water bath is colored black
to see the tissue easily
working temp of flotation water bath
5-10C below the melting point of wax
flotation water bath contains ? L of water
2
glass stoppered jars provided at the bottom pad with grooves,
coplin jars
coplin jars are used for (2)
fixation and staining
softens the floating tissues
flotation water bath
unfolds and flattens out wrinkled tissue sections on the water surface
flotation water bath
duration in stations 1&2
5 hours on first station
1 hour on 2nd
duration in stations 3-6
1 hour each for dehydration
duration in stations 7-8
1 hour each for further dehydration
duration in stations 9-10
1 hour each for clearing
duration in stations 11-12
1 hour each for paraffin infiltration or impregnation