GPHT LAB - Apparatus II

Question 1

Shape of microtome knife

two sides: one flat and one concave

Answer 1

plane-concave

Question 2

in plane concave knife, the one with lesser degree of concavity is used for __

Answer 2

celloidin section

Question 3

in plane concave knife, the one with more degree of concavity is used for __

Answer 3

paraffin sections on sledge, rotary or rocking microtome

Question 4

Shape of microtome knife

routinely used for frozen sections and extremely hard specimens embedded in paraffin wax

Answer 4

plane wedge

Question 5

Shape of microtome knife

used for paraffin blocks being cut on the rotary microtome

Answer 5

plane wedge

Question 6

length of plane wedge

Answer 6

100 mm

Question 7

when not in use, where must the knife be stored

Answer 7

in its box

Question 8

never lay the knife ___ on the bench

Answer 8

flat

Question 9

never allow the knife to become badly “ ___ “

Answer 9

nicked

Question 10

it is advisable to have a 2nd knife for ___ and ___

Answer 10

cutting bone and hard tissue

Question 11

the edge of the knife should be “___” daily before use

Answer 11

touch up

Question 12

always use a ___, when required for sharpening

Answer 12

back

Question 13

using a back for sharpening not necessary for what knives?

Answer 13

biconcave

Question 14

cleaning material for the knife

Answer 14

xylene or toluene

Question 15

never __ or __ a knife

Answer 15

lend or borrow

Question 16

knife sharpener with a vibrating frosted glass plate

Answer 16

automatic knife sharpener

Question 17

in the automatic knife sharpener, every __ strokes the blade is automatically turned over

Answer 17

4

Question 18

automatic knife sharpener require the use of ___

Answer 18

abrasives

Question 19

also called Cold Microtome

Answer 19

Cryostat

Question 20

a cold chamber, which is kept at -20C

Answer 20

Cryostat

Question 21

cold chamber of cryostat contains what microtome type

Answer 21

rotary

Question 22

average temperature of the cold chamber/cryostat

Answer 22

-20C

Question 23

used to cut frozen tissue

Answer 23

cryostat

Question 24

replaced the carbon dioxide microtome for frozen sections

Answer 24

cryostat

Question 25

thickness of tissue that can be cut by a cryostat

Answer 25

1-4 u

Question 26

apparatus to prepare frozen tissue

Answer 26

cryostat

Question 27

apparatus used to determine if tumor is benign or maignant

Answer 27

cryostat

Question 28

RFS takes how many min

Answer 28

15 - 20

Question 29

reagent in stations 1&2 of autotechnicon

Answer 29

10% formalin

Question 30

an automatic tissue processor that performs fixation, dehydration clearing and paraffin infiltration

Answer 30

autotechnicon

Question 31

adv of autotechnicon

Answer 31

constant agitation

Question 32

steps performed by autotechnicon (4)

Answer 32

fixation, dehydration, clearing and paraffin infiltration

Question 33

autotechnicon has how many contianers

Answer 33

12

Question 34

reagent in stations 3-6of autotechnicon

Answer 34

ascending grades of ethyl alcohol (70% to 95%)

Question 35

reagent in stations 7-8 of autotechnicon

Answer 35

2 changes of acetone

Question 36

reagent in stations 9-10 of autotechnicon

Answer 36

2 changes of chloroform or xylol

Question 37

reagent in stations 11&12 of autotechnicon

Answer 37

2 changes of liquid paraffin

Question 38

parts of autotechnicon

for the specimen

Answer 38

sample basket

Question 39

parts of autotechnicon

contains reagents

Answer 39

receptacle

Question 40

parts of autotechnicon

enables the operator to delay the beginning of the processing cycle for any desired time up to 60hours

Answer 40

delay timer

Question 41

parts of autotechnicon

contains power switch, selector, pilot light, transparent plastic door with lock and key

Answer 41

timing disc panel

Question 42

parts of autotechnicon

temp at +/- 0.7C controlled by a thermostat

Answer 42

melting pots