GPHT LAB - Apparatus II Flashcards
Shape of microtome knife
two sides: one flat and one concave
plane-concave
in plane concave knife, the one with lesser degree of concavity is used for __
celloidin section
in plane concave knife, the one with more degree of concavity is used for __
paraffin sections on sledge, rotary or rocking microtome
Shape of microtome knife
routinely used for frozen sections and extremely hard specimens embedded in paraffin wax
plane wedge
Shape of microtome knife
used for paraffin blocks being cut on the rotary microtome
plane wedge
length of plane wedge
100 mm
when not in use, where must the knife be stored
in its box
never lay the knife ___ on the bench
flat
never allow the knife to become badly “ ___ “
nicked
it is advisable to have a 2nd knife for ___ and ___
cutting bone and hard tissue
the edge of the knife should be “___” daily before use
touch up
always use a ___, when required for sharpening
back
using a back for sharpening not necessary for what knives?
biconcave
cleaning material for the knife
xylene or toluene
never __ or __ a knife
lend or borrow
knife sharpener with a vibrating frosted glass plate
automatic knife sharpener
in the automatic knife sharpener, every __ strokes the blade is automatically turned over
4
automatic knife sharpener require the use of ___
abrasives
also called Cold Microtome
Cryostat
a cold chamber, which is kept at -20C
Cryostat
cold chamber of cryostat contains what microtome type
rotary
average temperature of the cold chamber/cryostat
-20C
used to cut frozen tissue
cryostat
replaced the carbon dioxide microtome for frozen sections
cryostat
thickness of tissue that can be cut by a cryostat
1-4 u
apparatus to prepare frozen tissue
cryostat
apparatus used to determine if tumor is benign or maignant
cryostat
RFS takes how many min
15 - 20
reagent in stations 1&2 of autotechnicon
10% formalin
an automatic tissue processor that performs fixation, dehydration clearing and paraffin infiltration
autotechnicon
adv of autotechnicon
constant agitation
steps performed by autotechnicon (4)
fixation, dehydration, clearing and paraffin infiltration
autotechnicon has how many contianers
12
reagent in stations 3-6of autotechnicon
ascending grades of ethyl alcohol (70% to 95%)
reagent in stations 7-8 of autotechnicon
2 changes of acetone
reagent in stations 9-10 of autotechnicon
2 changes of chloroform or xylol
reagent in stations 11&12 of autotechnicon
2 changes of liquid paraffin
parts of autotechnicon
for the specimen
sample basket
parts of autotechnicon
contains reagents
receptacle
parts of autotechnicon
enables the operator to delay the beginning of the processing cycle for any desired time up to 60hours
delay timer
parts of autotechnicon
contains power switch, selector, pilot light, transparent plastic door with lock and key
timing disc panel
parts of autotechnicon
temp at +/- 0.7C controlled by a thermostat
melting pots
