BB LAB - Glossary II Flashcards
fusion of malignant and normal cell that produces large quantities of monoclonal antibodies
murine hybridoma
group of sugars found on rbc membrane attached to a protein backbone
NANA (Sialic acid)
major source of membrane’s net negative charge
NANA (Sialic acid)
relating to birth
natal
before birth
prenatal
after birth
postnatal
newborn infant up to 4 months of age
neonate
enzyme that cleaves sialic acid from rbc membrane
neuraminidase
also known as agglutination-inhibition
Neutralization
refers to absence of agglutination due to inability of an Ab to react with red cell antigen because of previously bound soluble substance
Neutralization
principle involved in saliva testing
Neutralization
basic building blocks of DNA composed of phosphate group, sugar group and base
nucleotide
any individual who fails to express any antigens on red cells
null phenotype
chemical compound formed by a small number of simple carbohydrate molecules
oligosaccharide chain
required temp in C by which the antibody is most reactive
optimum temperature
temp for IgM cold reactive antibody
1-6C/20-24C
IgG warm reacting antibody
37C
spontaneous clumping of rbc against a given serum
panagglutination
group O rgt RBC used for Ab ientification
panel cell
refers to a woman who had at least one pregnancy
primiparous
type of cold autoimmune hemolytic anemia in w/c a biphasic IgG Ab can be demostrated with anti-P specificity
Paroxysmal cold hemoglobinuria
D antigen thatis missing part of its typical antigenic structure
Partial D
refers to the chance that the alleged father is the biological father
Paternity index
diagrammatic chart for illustrating inheritance patterns of traits in a family study
Pedigree chart
detectable characteristics of genes
phenotype
initial treatment of elevated bilirubin using lights
phototherapy
extra blood sample collected during whole blood donation from a donor w/c is intended for serological test
pilot tube
fibrinogen containing liquid portion of anticoagulated blood
plasma
can be used as specimen for backward typing to detect antibodies however might present problem because it can bring about rouleaux formation
plasma
plasma prepared from whole blood subjected to heavy spin wc allow sedimentation of PLT leaving a plasma almost free of PLT
platelet poor plasma
plasma prepared from whole blood subjected to SOFT spin wc allow sedimentation of PLT leaving a plasma with high number of PLT
platelet rich plasma
products that are transfused in patients suffering from hypovolemia (dec plasma volume) or indicated among burn and shock patients
plasma volume expander
NSA meaning
normal serum albumin
PPF meaning
plasma protein fraction
NSA alb and globulin %
96% Alb + 4% Globulin
PPF alb and globulin %
83% Alb + 17% Globulin
Synthetic volume expanders
Crystalloids and Colloids
plasma derived volume expanders
NSA and PPF
Crystalloids [2]
Ringer’s Lactate (Na, Cl, K, Ca, lactate ions)
Normal Saline Solution (0.85%-0.9% NaCl)
Colloids [2]
Dextran (6%-10%)
HES (Hydroethyl starch)
inability of patient to respond favorably after PLT transfusion thus desired increment in PLT not achieved
PLT Refractoriness
spontaneous agglutination of red cells by most normal adult sera regardless of blood group
polyagglutination
blood group system showing several allelic forms therefore rendering more than one alternative phenotype
polymorphism
refers to after child birth
postpartum
refers to after tranfusion
posttransfusion
relative strength of Ab present in given antiserum usually expressed in titer
potency
any substance that enhances Ag-Ab interaction from a given cell-serum mixture w/c may be used to detect low-titered Ab in routine testing
potentiator
0.2% in NaCl in glycine
LISS (Low Ionic Strength Saline Solution)
this medium has lower ionic strength that increases rate of antibody association
incubation may be shortened to 5-15 min if this medium is used
LISS (Low Ionic Strength Saline Solution)
positively charge polymer that reduces zeta potential by neutralizing the negative charge in red cell
polybrene
useful in identification of Ab in Kidd blood group system
polybrene
enhances Ab-Ag rxn by concentrating Ab in the mixture through water removal
Polyethylene glycol
PEG meaning
Polyethylene Glycol
refers to 2x2 grid used for gene combination
punnett square
system of procedures that ensures a lab to operate with most minimal errors
quality assurance
close monitoring of lab operations to allow maximum reliability of test result
quality control
in transfusion medicine, this refers to a person who benefits and receives blood transfusion
recipient
refers to the required speed in a given time to sediment particles from a fluid mixture
relative centrifugal force
RCF value
1.118 x 10^5 x radius x (rpm)^2
RCF meaning
Relative centrifugal force
