GPHT LAB - Dehydration

Question 1

hastens dehydration process

Answer 1

heat

Question 2

reagent that ensures complete dehydration

Answer 2

anhydrous copper sulfate

Question 3

reagent which absorbs any water present

Answer 3

anhydrous copper sulfate

Question 4

color change for anhydrous copper sulfate

Answer 4

colorless to whitish blue

Question 5

commonly used solutions for dehydration

Answer 5

alcohol, acetone, dioxane

Question 6

for delicate tissues, at what concentration must dehydration start

Answer 6

30%

Question 7

also called grain alcohol

Answer 7

ethyl alcohol

Question 8

also called wood alcohol

Answer 8

methyl alcohol

Question 9

also called rubbing alcohol

Answer 9

isopropyl alcohol

Question 10

alcohol: not expensive, not toxic

Answer 10

ethyl alcohol

Question 11

what smears, use of MetOH

Answer 11

bone marrow and blood smears

Question 12

disadv of MetOH

Answer 12

toxic, can cause blindness

Question 13

disadv of Butyl alcohol

Answer 13

slow acting and not routinely used in lab

Question 14

what smears, use of isopropyl alcohol

Answer 14

compression smears

Question 15

use of Butyl alcohol

Answer 15

plant and animal microtechniques

Question 16

used for most urgent biopsies

Answer 16

acetone method

Question 17

rapid dehydrant but causes excessive shrinkage

Answer 17

acetone

Question 18

length of dehydration when using acetone

Answer 18

1/2 to 2 hours

Question 19

unique reagent

Answer 19

dioxane/ diethylene dioxide

Question 20

dual purpose of dioxane/ diethylene dioxide

Answer 20

dehydrating and clearing agent

Question 21

miscible with both water and molten paraffin wax

Answer 21

dioxane

Question 22

Disadv of __
highly toxic
expensive
sections prone to fall out of surrounding wax

Answer 22

dioxane

Question 23

should be used only in a well-ventilated lab

any residue should be washed down the sink

Answer 23

dioxane

Question 24

excellent dehydrating and clearing agent

Answer 24

dioxane

Question 25

2 Methods for dioxane

Answer 25

Weiseberger’s

Graupner’s

Question 26

also a dehydrating and clearing agent aside from dioxane

Answer 26

tetrahydrofuran

Question 27

can dissolve many substances including fats

Answer 27

tetrahydrofuran

Question 28

miscible with lower alcohols, ether, chloroform, acetone, benzene, xylene

Answer 28

THF

Question 29

less shrinkage and easier cutting of sections with fewer artifacts

Answer 29

THF

Question 30

does not dissolve out aniline dyes

Answer 30

THF

Question 31

most staining procedures give improved results with ___

Answer 31

THF

Question 32

used as a dehydrating agent because of its rapid action without producing overhardening and distortion of tissues

Answer 32

cellusolve

Question 33

combustible at 110F

Answer 33

cellusolve

Question 34

Cellusolve is combustible at ___

Answer 34

110F

Question 35

Disadv of cellusolve

Answer 35

highly toxic

can affect reproductive organs

Question 36

used as a dehydrating agent as it removes water very rapidly and produces very little distortion and hardening of tissue

Answer 36

tri-ethyl phosphate

Question 37

soluble in alcohol, ether, benzene, chloroform, acetone, xylene

Answer 37

tri-ethyl phosphate

Question 38

produces minimum tissue shrinkage

Answer 38

tri-ethyl phosphate

Question 38

Used both as dehydration and clearing agent

Answer 38

Dioxane

Tetrahydrofuran

Question 39

Soluble in many substances like alcohol, ether, benzene, etc

Answer 39

Tetrahydrofuran

Tri-ethyl phosphate

Question 40

Has fewer artifacts when used

Answer 40

Tetrahydrofuran

Question 41

Another name for cellusolve

Answer 41

Ethylene glycol monoethylether