CM LEC - CSF up to CSF Protein Flashcards

1
Q

CSF produced by

A

choroid plexus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

choroid plexus produces how many percent of CSF

A

70%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

where does the 30% CSF come from

A

plasma filtration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

provides nutrients to CNS

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

channel for removal of waste products

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

mechanical buffer

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

protects brain and spinal cord from sudden pressure changes

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

CSF is also called __

A

fluid under pressure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

volume regulator

A

CSF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

normal CSF volume baby

A

10-60 ml

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

normal CSF volume adult

A

140 - 170 ml

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

rate of formation

A

20 ml CSF/hour

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

gold std for diagnosis of meningitis

A

CSF analysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

3 layers of meninges

A

dura
arachnoid
pia mater

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

types of inflammation

A

septic

aseptic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

type of inflammation

viral, bacterial cause or other infectious agents

A

septic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

types of inflammation

due to trauma

A

aseptic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

methods of CSF collection

A

lumbar, cisternal, ventricular puncture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

methods of CSF collection

w/in 3rd, 4th, 5th lumbar vertebrae

A

lumbar puncture
spinal puncture
lumbar tap

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

methods of CSF collection

most common

A

lumbar puncture
spinal puncture
lumbar tap

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

methods of CSF collection
used among infants only
done while baby’s fontanelle is open

A

ventricular puncture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

methods of CSF collection

at base of the skull, not popular

A

cisternal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

too much sx can’t be collected unless the pressure is

A

normal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

opening pressure for adult

A

90-180 mm H20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

opening pressure for young

A

10 - 100 mm H20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

2 materials mentioned, used for csf collection

A

L-shaped adaptor (where needle is attached)

sterile manometer tube (measures pressure)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

when can doctor aspirate?

A

when pressure is constant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

closing pressure

A

45 -90 mm H20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

when pressure suddenly drops

A

subarachnoid blockage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

when pressure suddenly drops, do not ___

A

aspirate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

determines the subarachnoid block which lowers the opening pressure

A

Queckenstendt test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

CSF is always considered a STAT specimen except for vial # ?

A

2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

vial # 1 is for what section

A

Chemistry/Serology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

vial # 2 is for what section

A

Bacteriology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

vial # 3 is for what section

A

Hematology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

at what temp should vial # 1 be kept

A

freezing temp/freezer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

at what temp should vial # 2 be kept

A

RT/incubator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

at what temp should vial # 3 be kept

A

refrigerator temp

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

routine tests done on csf in chem section

A

CSF glucose

CSF protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

special tests done on csf in chem section

A
CSF CK - MB
CSF CK -MT
CSF LDH
Glutamine
Lactate
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

tests done on csf in serology section

A

vDRL
FTA - ABS
CSF C-RP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

vDRL meaning

A

veneral disease research laboratory

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

FTA-ABS meaning

A

Fluorescent Treponemal Antibody Absorption Test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

tests done on csf in bacte section

A

G/S
C/S
AFB
India Ink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

tests done on csf in hema section

A

WBC Count

WBC Differential Count

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

RBC in CSF indication of

A

traumatic puncture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

physical analysis is done on w/c section

A

hema

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

chemical analysis is done on w/c section

A

cc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

microscopic analysis is done on w/c section

A

bacte

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

possible appearance of CSF (5)

A
crystal clear
cloudy, milky, turbid
xanthochromic
clotted
pellicle
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

normal appearance of csf

A

colorless and clear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
52
Q

CSF comparable to _

A

water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
53
Q

blank used for CSF analysis

A

distilled water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
54
Q

appearance of CSF

indicates no cell count

A

clear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
55
Q

appearance of CSF

indicates moderate cell count

A

cloudy/hazy

56
Q

appearance of CSF

indicates high cell count

A

turbid

57
Q

purpose of dilution of sx prior to counting

A

so cells are not overlapping

58
Q

appearance of CSF

pale orange/ reddish/ yellowish discoloration

A

xanthochromic

59
Q

xanthochromic sx has the presence of (4)

A

bilirubin, carotene, Hgb, protein

60
Q

appearance of CSF

indicates traumatic puncture

A

clotted

61
Q

appearance of CSF

tiny white aggregates on sample surface

A

pellicle

62
Q

pellicle also described as __

A

cobweb or tigerweb

63
Q

appearance of CSF

correlated with TB meningitis

A

pellicle

64
Q

specimen suspected with __ and __ should not be kept in fridge

A

H. influenzae and N. meningitis

65
Q

specimen suspected with H. influenzae and N. meningitis

because these are __

A

labile, killed at certain temp

66
Q

Bloody specimen can have 2 causes

A

Traumatic aspiration

True intracranial bleeding

67
Q

distribution of RBCs in Traumatic aspiration

A

uneven

68
Q

distribution of RBCs in True intracranial bleeding

A

even

69
Q

Presence of clot in TA

A

+ due to plasma contamination

70
Q

Presence of clot in TIB

A

-

71
Q

Xanthochromia in TA

A

clear

72
Q

Xanthochromia in TIB

A

xanthochromic

73
Q

CSF CK-BB in TA

A

not mentioned

74
Q

CSF CK-BB in TIB

A

higher

75
Q

Isoenzymes of CK

A

CK - MM
CK - BB
CK - MB

76
Q

CSF Lactate in TA

A

not mentioned

77
Q

CSF Lactate in TIB

A

increased

78
Q

when does lactate increase

A

during tissue destruction

79
Q

transparency of csf

A

clear

80
Q

sp gr of csf

A

1.006 - 1.008

81
Q

pH of csf

A

7.30 - 7.35

82
Q

WBC Count Normal Values for adult

A

0-5WBC/ ul

83
Q

WBC Count Normal Values for infants

A

0-30 WBC/ ul

84
Q

increased WBC in CSF

A

pleocytosis

85
Q

if sx is clear
what is the dilution
manner of counting
where to count/ what formula to use

A

no dilution
direct counting
count in 9 large squares

86
Q

if sx is hazy
what is the dilution
manner of counting
where to count/ what formula to use

A

1:10 dilution
count in 5 large squares
use std formula for actual wbc count

87
Q

if sx is turbid
what is the dilution
manner of counting
where to count/ what formula to use

A

1:20
count in 4 large squares
use std formula for actual wbc count

88
Q

diluting fluid for CSF WBC count

A

Tuerk’s

3% Acetic Acid

89
Q

what tube is used in charging for CSF WBC count

A

plain blue capillary tube

90
Q

manner of mixing for CSF WBC count

A

inversion

91
Q

for CSF WBC count, may be counted as a wbc

A

nucleated red cell

92
Q

methods of obtaining sediment for diff count

A

sedimentation
filtration
cytocentrifugation
centrifugation

93
Q

most common, most backward method of obtaining sediment for diff count

A

sedimentation

94
Q

what to d with supernatant after sedimentation

A

give to chem section

95
Q

stain used in CSF diff count

A

wright stain

96
Q

advanced method of obtaining sediment for diff count

A

cytocentrifugation

97
Q

in diff count, if cells counted do not reach 100, report only the ___ seen

A

number of cell types seen

98
Q

method of obtaining sediment for diff count w/ good cell recovery

A

cytocentrifugation

99
Q

method of obtaining sediment for diff count w/ complete cell recovery

A

filtration

100
Q

stain for blood cells

A

wright

101
Q

stain for cancer cells

A

papanicolaou

102
Q

stain for parasitic cells

A

giemsa

103
Q

CSF Cell constituents and %

A

70% lympho

30% mono

104
Q

> 70% PMN indicates

A

bacteria meningitis

105
Q

Early viral, fungal or tubercular meningitis demonstrates increase in what cells

A

PMN

106
Q

Chronic viral, fungal or tubercular meningitis demonstrates increase in what cells

A

Lympho

107
Q

parasitic meningitis demonstrates increase in what cells

A

eosino

108
Q

bacterial meningitis demonstrates increase in what cells

A

PMN

109
Q

Immature leukocytes indicates

A

leukemia

110
Q

malignant cells indicates

A

tumor/cancer

111
Q

plasma cells and reactive lympho indicates

A

HIV/ AIDS

112
Q

macrophages in brain

A

microglial cells

113
Q

bacteria that causes increase in lympho

A

Listeria monocytogenes

114
Q

fungi with star burst appearance

A

C. neoformans

115
Q

N.V. of CSF protein

A

15 - 45 mg/dL

116
Q

Elevated CSF protein indicates

A

damage in BBB

117
Q

why CSF protein is

A

CSF protein is a product of filtration, proteins cannot pass the filtering membrane

118
Q

conditions of increased CSF protein

A

meningitis
traumatic tap
IgG synthesis

119
Q

in traumatic tap, a drop of plasma causes how much increase of protein

A

10x

120
Q

globulin produced during infection

A

IgG

121
Q

conditions of decreased CSF protein

A

Elevated intracranial pressure
large sample removal
hyperthyroidism
otorrhea

122
Q

CSF leaks from brain to ears

A

otorrhea

123
Q

CSF leaks from brain to nose

A

rhinorrhea

124
Q

protein fraction normally in csf (decreasing order)

A
Alb
Pre-alb
Ceruloplasmin
Transferrin
IgA
IgG
125
Q

Marker to differentiate CSF from other body fluids

A

Tau protein or T protein or carbohydrate deficient protein (?)

126
Q

proteins not normally in CSF

A

fibrinogen
IgM
beta lipoprotein

127
Q

precipitating agents (2)

A

SSA

TCA

128
Q

SSA meaning

A

sulfosalicylic acid

129
Q

TCA meaning

A

trichloroacetic acid

130
Q

can ppt alb fraction better than glob fraction

A

SSA

131
Q

can ppt alb fraction and glob fraction better

A

TCA

132
Q

more sensitive ppt agent

A

TCA

133
Q

Supernatant (Alb) measured using what dyes

A

BCG
MO
HABA

134
Q

Used to visualize all protein fractions

A

electrophoresis

135
Q

type of electrophoresis commonly used for CSF

A

PAGE

136
Q

first choice dye

has affinity for all protein fractions

A

Coomassie Brilliant Blue