Epithelial Tissue - Histo Lec Flashcards
aggregates or groups of cells organized to perform one or more specific functions.
tissues
Four basic types of tissues:
– covers body surfaces, lines body cavities and forms glands
Epithelium
Four basic types of tissues:
underlies or supports the other three basic tissues, both structurally and functionally.
Connective
Four basic types of tissues:
made up of contractile cells and is responsible for movement
Muscle
Four basic types of tissues:
receives, transmits, and integrates information from outside and inside the body to control activities of the body.
Nerve
Avascular tissues
epithelial tissue
Covers body surfaces,
As receptors for the special senses
epithelial tissue
lines body cavities,
epithelial tissue
and constitutes glands;
epithelial tissue
Sheet of cells lying close together
epithelial tissue
Continuous sheet w/ little extracellular matrix
epithelial tissue
Function of Epithelial Tissue
a. Diffusion of O2 and CO2 across the epithelial cells of lung alveoli and capillaries
Transcellular Transport
Function of Epithelial Tissue
b. Carrier-protein mediated transport of amino acids
Transcellular Transport
Function of Epithelial Tissue
c. Vesicle-mediated transport of IgA and other molecules
Transcellular Transport
Function of Epithelial Tissue
Occurs via endocytosis and pinocytosis (columnar epithelium of the intestines and proximal convoluted tubules in the kidney)
Absorption
Function of Epithelial Tissue
Occurs via exocytosis (different glands produce perspiration, oil, digestive enzymes and mucus)
Secretion
Function of Epithelial Tissue
From abrasion and injury by the epithelial layer of the skin and gall bladder.
Protection
Function of Epithelial Tissue
Lining of Kidney tubules filtering wastes from blood plasma
Filtration
Structure of simple squamous
Single Layer of flattened cells
Function of simple squamous
Absorption, and filtration
Not effective protection – single layer of cells
Location of simple squamous
Walls of capillaries, air sacs in lungs
Form serous membranes in body cavity
Mesothelium (lining of peritoneum and pleura )
Endothelium (lining of blood vessels)
Structure of simple cuboidal
Single layer of cube shaped cells
Function of simple cuboidal
Secretion and transportation in glands, filtration in kidneys
Location of simple cuboidal
Glands and ducts (pancreas & salivary), kidney tubules, covers ovaries
Structure of simple columnar
Elongated layer of cells with nuclei at same level
Function of simple columnar
Absorption, Protection & Secretion
Special Features of simple columnar
Microvilli, finger-like projections of the apical surface, increase surface area for absorption
Goblet cells, single cell glands, produce protective mucus.
Location of simple columnar
Linings of entire digestive tract, gall bladder, uterus, kidney tubules
When open to body cavities, simple columnar are called ______
mucous membranes
Structure of pseudostratified
Irregularly shaped cells with nuclei at different levels – appear stratified, but aren’t.
All cells reach basement membrane
Function of pseudostratified
Absorption and Secretion
Features of pseudostratified
Goblet cells produce mucus
Cilia – hair-like projections; move fluid over cell surface or confer cell motility (larger than microvilli)
Location of pseudostratified
Respiratory Linings & Reproductive tract
Structure of stratified squamous
Several layer of cells with :
a. flattened cells (upper layer); b. polyhedral cells (middle layer) c. columnar cells (basal layer).
Types of stratified squamous
Stratified squamous non-keratinizing – flattened surface cells retain their nuclei; found in vagina, esophagus and tongue.
Stratified squamous keratinizing – flattened surface cells lose their nuclei, form a layer of dead keratinized cells; found in the skin
Also known as Urothelium
transitional epithelium
Location of transitional epithelium
Renal pelvis, urinary bladder, ureters & proximal 1/3 of urethra.
Function of transitional epithelium
Allows stretching (change size)
Structure of transitional epithelium
Many layers
Superficially located umbrella-shaped cells; pyriform cells in the middle layer and columnar or polyhedral cells in the most basal layer.
rare cube-shaped commonly cells make up two layers protection of larger ducts: pancreas, salivary, and sweat glands mammary gland in the breast
STRATIFIED CUBOIDAL
Basal cells are typically cuboidal with surface cells either columnar or cuboidal in appearance
STRATIFIED CUBOIDAL
rare
secretion and protection
intestinal lining - ciliated to help move nutrients and increase absorption, also protects against pathogens/bacteria
STRATIFIED COLUMNAR
often found between simple columnar epithelia and stratified squamous epithelia
STRATIFIED COLUMNAR
found near salivary glands - cell lining protects the salivary duct, goblet cells found between the cells
STRATIFIED COLUMNAR
found in vas deferens where it protects and aids in secretion of glands
STRATIFIED COLUMNAR
also form layers in ocular conjunctiva and the linings of the pharynx, anus, uterus, urethra
STRATIFIED COLUMNAR
Three principal characteristics of epithelial tissue
a. Closely apposed and adhere to one another by means of specific cell-to-cell adhesion molecules (cell junctions)
b. Functional and morphologic polarity: three morphologic domains
c. Basement membrane
refers to the asymmetric organization of different aspects of the cell including the cell surface, intracellular organelles and the cytoskeleton
Polarity -
Features of Apical Surface of Epithelium
Finger-like extensions of the plasma membrane of apical epithelial cell
Increase surface area for absorption
microvilli
Features of Apical Surface of Epithelium
found in small intestine, kidney tubules
microvilli
Features of Apical Surface of Epithelium
Does not conform to the function of cilia
Stereocilia
Features of Apical Surface of Epithelium
Longer than microvilli but less motile
Stereocilia
Features of Apical Surface of Epithelium
Does not conform to the function of cilia
Stereocilia
location of Stereocilia
epididymis & ductus deferens
Features of Apical Surface of Epithelium
(eg.) respiratory tubes
Whip-like/hair-like projections, motile extensions
Moves mucus, etc. over epithelial surface 1-way (moves mucus upward but not downward)
Cilia:
Features of Apical Surface of Epithelium
(eg.) spermatozoa
Extra long cilia
Moves cell
Flagella:
Contain specialized junctions that contain cell adhesion molecules (CAMs) and restrict movement of materials into and out of lamina.
THE LATERAL DOMAIN
Membrane-associated structures that functions in cell-to-cell attachment of columnar epithelial cells.
JUNCTIONAL COMPLEX
Three distinct components of junctional complex
- Tight Junctions (zonula occludens)
- Anchoring Junctions
a. belt desmosomes, zonula adherens b. desmosome (macula adherens)
Formed by the fusion of the outer leaflets of cell’s plasma membrane
ex . Active transport-Prevents back-diffusion of transported substances
Tight Junctions (zonula occludens)
Junction for
Sealing off intercellular space
Tight Junctions (zonula occludens)
Prevents digested macromolecules passing thru adjacent cells (impermeable barrier)
Tight Junctions (zonula occludens)
Analogous to fascia occludens on adjacent endothelial cells lining capillaries
Tight Junctions (zonula occludens)
Has 4 transmembrane – tightly attach cellular membrane
-aid in attachment
Tight Junctions (zonula occludens)
Provides mechanical stability to group of epithelial cells
anchoring junctions
Provide lateral adhesions between epithelial cells
anchoring junctions
Cell Adhesion Molecules on both lateral and basal domain
anchoring junctions
- surrounds the entire perimeter of epithelial cells just basal to the tight junction
a. Zonula Adherens aka belt desmosomes
- contains adhesion molecule “E-cadherin”
a. Zonula Adherens aka belt desmosomes
feature of Zonula Adherens aka belt desmosomes
mediates attachment of E-cadherin
Extracellular Accessory Link/Ion (Ca2+)
feature of Zonula Adherens aka belt desmosomes
attached to the transmembrane link protein
Intracellular Link Protein
Localized spot-like junction between epithelial cells
Each has intracellular plaque
Macula Adherens “desmosome”
dense plaque of intercellular which consists of several link protein attachment proteins on cytoplasmic surface of each opposing cell.
desmoplakins
loop into and out of the desmoplakins
Keratin –
– transmembrane linker glycoproteins between adjacent cells mediates the attachment
Desmogleins and desmocollins
Allows selective diffusion of molecules; cell-cell communication
Gap Junctions Communicating junctions or nexus
Is not a part of the junctional complex
Gap Junctions Communicating junctions or nexus
Has pores for substance passage
Gap Junctions Communicating junctions or nexus
Common also in CNS, cardiac muscle, and smooth muscle pass signals involved in contraction from one cell to another
Gap Junctions Communicating junctions or nexus
Plaque-like(circular patch)entity composed of channels called “Connexons”
Gap Junctions Communicating junctions or nexus
– six cylindrical connexins around a central channel
Connexons
alter conformation to shut off communication between cells
Connexins -
visible only by E.M.
Basal Lamina
Consists of Matrix protein: type IV collagen, laminin, entactin + perlacan, and proteoglycans
Basal Lamina
composition of matrix protein of basal lamina
type IV collagen, laminin, entactin + perlacan, and proteoglycans
two zones of Basal Lamina
lies next to the plasma membrane
- lamina lucida (lamina rara) –
two zones of Basal Lamina
lies adjacent to the reticular lamina of deeper C.T.
- lamina densa –
Basal lamina + reticular lamina
Basement membrane
Attachment of epithelial cells to underlying support tissue
Basement membrane
Mediate adhesion of Epithelial cells to the underlying extracellular matrix
B. Hemidesmosomes
Found in basal surface of basal cells (tracheal epithelium and stratified squamous).
B. Hemidesmosomes
Dense cytoplasmic plaque linked via integrins to laminins in the basal lamina
B. Hemidesmosomes
Type VII collagen from the basal lamina extend deeper into the underlying connective tissue and insert into plaques of type IV collagen.
B. Hemidesmosomes
“Cell-to-Extracellular Matrix Junctions”
B. Hemidesmosomes