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Botany > Bacte Lec - Entero Part 2 > Flashcards

Bacte Lec - Entero Part 2 Flashcards

1
Q

LIA: Lysine decarboxylase +

A

Purple/purple

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2
Q

LIA: Lysine decarboxylase -

A

Purple/yellow

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3
Q

LIA: Deaminase positive

A

Red/Yellow

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4
Q

LIA: Blackening

A

H2S positive

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5
Q

IMViC

A

Indole Methyl red voges proskauer citrate test

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6
Q

tryptone is present in what cm

A

tryptone broth

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7
Q

when tryptone is utilized, what is the by product?

A

indole

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8
Q

+ result in indole tests

A

red ring when overlayed with Kovac’s or Ehrlich’s reagent

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9
Q

what is the Kovac’s or Ehrlich’s reagent

A

PDAB (Para-diethylaminobenzaldehyde or Para-dimethylaminobenzaldehyde)

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10
Q

if tryptone broth is not available, what to use?

A

SIM

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11
Q

enzyme in indole tests

A

tryptophanase

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12
Q
  • result in indole tests
A

yellow or brown ring [no tryptophanase]

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13
Q

glucose by products

A

acetoin

pyruvic acid

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14
Q

meaning of K/K on LIA

A

decarboxylation (purple/purple)

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15
Q

meaning of R/A on LIA

A

deamination (red/yellow)

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16
Q

when acetoin is oxidized by air or KOH, it is transformed to

A

diacetyl

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17
Q

diacetyl in MR test is determined by adding

A

VP reagent

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18
Q

what is the VP Reagent

A

5% alpha naphthol in absolute methyl alcohol

40% KOH w/ 0.3% creatine

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19
Q

+ result in VP test

A

pink-red color [unstable]

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20
Q

MR & VP cannot be both [what result + or -]

A

cannot be both +

21
Q

color of methyl red in acidic solutions

A

red

22
Q

mixed acid fermentation makes the medium

A

acidic, pH less than 4.4

23
Q

pH range of methyl re

A

4-6

24
Q

+ result in MR test

A

red color

25
Q

culture medium of citrate utilizaton test

A

simmon citrate agar

26
Q

principle of citrate utilizaton test

A

based on degradation of citrate

27
Q

indicator of sca

A

bromthymol blue

28
Q

+ result in citrate utilizaton test

A

blue [means citrate is degraded]

29
Q
  • result in citrate utilizaton test
A

green

30
Q

rep organism for gram (-)

A

Escherichia coli

31
Q

rapid urease producing organisms

A

Proteus, Campylobacter, H. pylori

32
Q

best way to differentiate enterobacter and klebsiella

A

motility test

33
Q

best motility test

A

hanging drop

34
Q

identify organism
LF
GMS on emb
IMViC Test: ++–

A

escherichia

35
Q

identify organism
LF
dark purple on emb
IMViC Test: –++

A

enterobacter

klebsiella

36
Q 
less mucoid
urease negative
motile
w/o capsule
center [fish eye]
A

enterobacter

37
Q

mucoid more
urease +
nonmotile
w/ capsule

A

klebsiella

38
Q

nonlactose fermenter, spreading/swarming

A

Pseudomonas

Proteus/Morganella

39
Q

nonlactose fermenter, spreading/swarming, pigmented,

A

pseudomonas

40
Q

reaction of pseudomonas in TSI

A

K/K

41
Q

nonlactose fermenter, spreading/swarming, non-pigmented,

A

Proteus

Morganella

42
Q

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A

A

Morganella morganii

43
Q

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +

A

P. vulgaris or P. mirabilis

44
Q

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol +

A

P. vulgaris

45
Q

nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol -

A

P. mirabilis

46
Q

odor of proteus

A

burned gunpowder

47
Q

odor of pseudomonas

A

fruity

48
Q

+ result in urease test

A

red color

49
Q
  • result in urease test
A

salmon/yellow color

Botany (324 decks)

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