Bacte Lec - Entero Part 2 Flashcards
LIA: Lysine decarboxylase +
Purple/purple
LIA: Lysine decarboxylase -
Purple/yellow
LIA: Deaminase positive
Red/Yellow
LIA: Blackening
H2S positive
IMViC
Indole Methyl red voges proskauer citrate test
tryptone is present in what cm
tryptone broth
when tryptone is utilized, what is the by product?
indole
+ result in indole tests
red ring when overlayed with Kovac’s or Ehrlich’s reagent
what is the Kovac’s or Ehrlich’s reagent
PDAB (Para-diethylaminobenzaldehyde or Para-dimethylaminobenzaldehyde)
if tryptone broth is not available, what to use?
SIM
enzyme in indole tests
tryptophanase
- result in indole tests
yellow or brown ring [no tryptophanase]
glucose by products
acetoin
pyruvic acid
meaning of K/K on LIA
decarboxylation (purple/purple)
meaning of R/A on LIA
deamination (red/yellow)
when acetoin is oxidized by air or KOH, it is transformed to
diacetyl
diacetyl in MR test is determined by adding
VP reagent
what is the VP Reagent
5% alpha naphthol in absolute methyl alcohol
40% KOH w/ 0.3% creatine
+ result in VP test
pink-red color [unstable]
MR & VP cannot be both [what result + or -]
cannot be both +
color of methyl red in acidic solutions
red
mixed acid fermentation makes the medium
acidic, pH less than 4.4
pH range of methyl re
4-6
+ result in MR test
red color
culture medium of citrate utilizaton test
simmon citrate agar
principle of citrate utilizaton test
based on degradation of citrate
indicator of sca
bromthymol blue
+ result in citrate utilizaton test
blue [means citrate is degraded]
- result in citrate utilizaton test
green
rep organism for gram (-)
Escherichia coli
rapid urease producing organisms
Proteus, Campylobacter, H. pylori
best way to differentiate enterobacter and klebsiella
motility test
best motility test
hanging drop
identify organism
LF
GMS on emb
IMViC Test: ++–
escherichia
identify organism
LF
dark purple on emb
IMViC Test: –++
enterobacter
klebsiella
less mucoid urease negative motile w/o capsule center [fish eye]
enterobacter
mucoid more
urease +
nonmotile
w/ capsule
klebsiella
nonlactose fermenter, spreading/swarming
Pseudomonas
Proteus/Morganella
nonlactose fermenter, spreading/swarming, pigmented,
pseudomonas
reaction of pseudomonas in TSI
K/K
nonlactose fermenter, spreading/swarming, non-pigmented,
Proteus
Morganella
nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A
Morganella morganii
nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +
P. vulgaris or P. mirabilis
nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol +
P. vulgaris
nonlactose fermenter, spreading/swarming, non-pigmented, TSI = K/A +, indol -
P. mirabilis
odor of proteus
burned gunpowder
odor of pseudomonas
fruity
+ result in urease test
red color
- result in urease test
salmon/yellow color
