BACTE LEC - Non-fermenting Gram (-) Bacilli to Pseudomonadaceae

Question 1

O2 requirements of Non-fermenting Gram (-) Bacilli

Answer 1

AEROBIC

Question 2

Fail to acidify OF media overlaid with Mineral oil and TSI

Answer 2

Non-fermenting Gram (-) Bacilli

Question 3

Where are Non-fermenting Gram (-) Bacilli found

Answer 3

in most environments: ubiquitous

Question 4

can withstand treatment with disinfectants

Answer 4

Non-fermenting Gram (-) Bacilli

Question 5

resistant to most antimicrobials

Answer 5

Non-fermenting Gram (-) Bacilli

Question 6

O2 requirements of Pseudomonas

Answer 6

strict aerobes

Question 7

motility of Pseudomonas

Answer 7

motile

Question 8

oxidase rxn of Pseudomonas

Answer 8

+ except for P. luteolus and P. oryzihabitans

Question 9

Catalase rxn of Pseudomonas

Answer 9

+

Question 10

cm for most Pseudomonas

Answer 10

MAC

Question 11

Most are oxidizers, some assacharolytic, what family

Answer 11

Pseudomonas

Question 12

most frequently isolated spp of Pseudomonas

Answer 12

P. aeruginosa

Question 13

common cause of nosocomial infections

Answer 13

P. aeruginosa

Question 14

CA of bacteremia, skin, wound infections, UTIs, endocarditis

Answer 14

P. aeruginosa

Question 15

CA of swimmer’s ear

Answer 15

P. aeruginosa

Question 16

CA of jacuzzi/ hot tub syndrome

Answer 16

P. aeruginosa

Question 17

CA of severe pneumonia among CF patients

Answer 17

P. aeruginosa

Question 18

Virulence Factors of P. aeruginosa

Answer 18

LPS
Unusual porin channels
Pili
Capsule [Alginate]
Elastase, Collagenase, Protease
Exotoxin A
Hemolysin
Pyocyanin pigment

Question 19

Rx of choice for P. aeruginosa

Answer 19

Aminoglycosides
Piperacillin
Ticarcillin
Ceftazidine
Cefepime

Question 20

pigments of P. aeruginosa [4]

Answer 20

pyocyanin
pyoverdin
pyorubin
pyomelanin

Question 21

odor of P. aeruginosa

Answer 21

grape like or tortilla like

Question 22

temperature for P. aeruginosa growth

Answer 22

able to grow at 42C

Question 23

Describe appearance of P. aeruginosa on BAP

Answer 23

Ground glass appearance: large, rough, dull grayish colonies w/ feathery edge

Question 24

hemolytic rxn of P. aeruginosa on BAP

Answer 24

beta

Question 25

appearance of P. aeruginosa on clear agars

Answer 25

visible pigment

Question 26

appearance of P. aeruginosa on EMB

Answer 26

Colorless [non-fermenting]

Question 27

TSI Rxn of P. aeruginosa

Answer 27

K/K

Question 28

Will P. aeruginosa grow in Cetrimide Agar

Answer 28

Yes

Question 29

2 Other Pseudomonas species
>normal flora, opportunistic
>low virulence
>assoc with UTIs, post surgical abscesses, empyema, septic arthritis

Answer 29

P. fluorescens and P. putida

Question 30

Appear as dry, wrinkled, with buff/ light brown color (tough and adherent)

Answer 30

Pseudomonas stutzeri

Question 31

Appear as non-wrinkled, flat colonies with yellowish brown pigment

Answer 31

Pseudomonas mendocina

Question 32

Other Pseudomonas spp
>soil denitrifiers: use ammonium as sole nitrogen source
>can grow in aerobic condition

Answer 32

Pseudomonas mendocina

Pseudomonas stutzeri

Question 33

Other Pseudomonas spp
>Usually contaminants
>

Answer 33

Pseudomonas alcaligenes

Pseudomonas pseudoalcaligenes

Question 34

recovered in Japanese rice paddies

Answer 34

P. oryzihabitans

Question 35

Other Pseudomonas spp
>Oxidase (-)
>Produce yellow non-diffusable pigments
>Colonies: wrinkled or rough

Answer 35

P. luteola

P. oryzihabitans