BACTE LEC - Non-fermenting Gram (-) Bacilli to Pseudomonadaceae Flashcards
O2 requirements of Non-fermenting Gram (-) Bacilli
AEROBIC
Fail to acidify OF media overlaid with Mineral oil and TSI
Non-fermenting Gram (-) Bacilli
Where are Non-fermenting Gram (-) Bacilli found
in most environments: ubiquitous
can withstand treatment with disinfectants
Non-fermenting Gram (-) Bacilli
resistant to most antimicrobials
Non-fermenting Gram (-) Bacilli
O2 requirements of Pseudomonas
strict aerobes
motility of Pseudomonas
motile
oxidase rxn of Pseudomonas
+ except for P. luteolus and P. oryzihabitans
Catalase rxn of Pseudomonas
+
cm for most Pseudomonas
MAC
Most are oxidizers, some assacharolytic, what family
Pseudomonas
most frequently isolated spp of Pseudomonas
P. aeruginosa
common cause of nosocomial infections
P. aeruginosa
CA of bacteremia, skin, wound infections, UTIs, endocarditis
P. aeruginosa
CA of swimmer’s ear
P. aeruginosa
CA of jacuzzi/ hot tub syndrome
P. aeruginosa
CA of severe pneumonia among CF patients
P. aeruginosa
Virulence Factors of P. aeruginosa
LPS Unusual porin channels Pili Capsule [Alginate] Elastase, Collagenase, Protease Exotoxin A Hemolysin Pyocyanin pigment
Rx of choice for P. aeruginosa
Aminoglycosides Piperacillin Ticarcillin Ceftazidine Cefepime
pigments of P. aeruginosa [4]
pyocyanin
pyoverdin
pyorubin
pyomelanin
odor of P. aeruginosa
grape like or tortilla like
temperature for P. aeruginosa growth
able to grow at 42C
Describe appearance of P. aeruginosa on BAP
Ground glass appearance: large, rough, dull grayish colonies w/ feathery edge
hemolytic rxn of P. aeruginosa on BAP
beta
appearance of P. aeruginosa on clear agars
visible pigment
appearance of P. aeruginosa on EMB
Colorless [non-fermenting]
TSI Rxn of P. aeruginosa
K/K
Will P. aeruginosa grow in Cetrimide Agar
Yes
2 Other Pseudomonas species
>normal flora, opportunistic
>low virulence
>assoc with UTIs, post surgical abscesses, empyema, septic arthritis
P. fluorescens and P. putida
Appear as dry, wrinkled, with buff/ light brown color (tough and adherent)
Pseudomonas stutzeri
Appear as non-wrinkled, flat colonies with yellowish brown pigment
Pseudomonas mendocina
Other Pseudomonas spp
>soil denitrifiers: use ammonium as sole nitrogen source
>can grow in aerobic condition
Pseudomonas mendocina
Pseudomonas stutzeri
Other Pseudomonas spp
>Usually contaminants
>
Pseudomonas alcaligenes
Pseudomonas pseudoalcaligenes
recovered in Japanese rice paddies
P. oryzihabitans
Other Pseudomonas spp
>Oxidase (-)
>Produce yellow non-diffusable pigments
>Colonies: wrinkled or rough
P. luteola
P. oryzihabitans
