Bacte Lab - Quiz 1 Flashcards
– materials containing all essential nutrients for bacterial growth
- → for bacterial growth
- culture media may be in granular or powdered form
Culture media
-food and soil (where it is planted) for bacteria
Culture media
for how long and at what temperature must the CM have for the bacteria to grow
35 – 37C, 18 – 24 hours
2 forms of CM
granular or powdered form
CM for motility
Sulfide-Indole-Motility (SIM)
Characteristics of Good Culture Media
- Sterile
- Free of inhibitory substances
- Adequate amount of Salt, Water & Certain Essential Nutrients
- Has a proper pH
Classif of CM accdg to physical state or consistency
solid
semi-solid
liquid
Classif of CM accdg to physical state or consistency
no solidifying agent
liquid
Classif of CM accdg to physical state or consistency
routinely used for bacterial cultivation
liquid
sign of bacterial growth in liquid media
turbidity
example of liquid media
broth
Classif of CM accdg to physical state or consistency
with solidifying agent
solid
most common solidifying agent
agar
examples of solidifying agents
agar
albumin
gelatin
solidifying agent that is used as a diagnostic medium
*Gelatin
agar is liquid at what temp
100C
agar is solid at what temp
38C
Gelatin is solid at what temp
25C
agar concentration of solid media
1.5-3%
agar conc of semi-solid media
0.5 - 1%
Classif of CM accdg to physical state or consistency
used to determine if bacteria is motile or not
semi-solid
classif based on application or function
basal/ordinary/simple enriched selective differential assay medium maintenance w/o glucose
classif based on application or function
useful for assaying vitamins, antibiotics, amino acids
assay medium
classif based on application or function
with inhibitory substances
selective
classif based on application or function
differentiates types of bacteria using dye or indicator
differential
classif based on application or function
supports growth of fastidious bacteria
enriching substances are added
enriched
classif based on application or function
basic medium from which you can prepare different media
Simple/Basal/Ordinary
classif based on application or function
supports the growth of non-fastidious bacteria
Simple/Basal/Ordinary
bacteria that grows immediately in a nutrient agar without added enriching substances
non-fastidious bacteria
enriching substances added:
- blood
- serum
- acidic fluid
examples of enriched media (3)
- Loeffler’s serum agar
- Chocolate Agar – contains RBCs
- Blood Agar
ex of differential media
EMB
MacConkey
Mannitol-Salt agar
indicator of Eosin-methylene blue agar
eosin-methylene blue
differential media used for for Escherichia coli (greenish metallic sheen)
EMB
differentiates lactose fermenters from non-lactose fermenters
EMB
colors of lactose fermenters
purple, red, pink
colors of non-lactose fermenters
white, colorless, yellow
indicator of MacConkey
neutral red
indicator of Mannitol-Salt agar
phenol red
differential media for growth of Staphylococcus aureus
-
Mannitol-Salt agar
differentiates Staphylococcus aureus from Staphylococcus epidermidis
Mannitol-Salt agar
selective media that inhibits growth of Gram (+):
- Gentian violet
- Na deoxycholate
- Bile salt
selective media that Inhibits growth of Gram (-)
- Na azide
* K tellurite
selective media which Prevents spreading of Proteus
- Chloral hydrate
* Alcohol
classif accdg to composition
synthetic
non-synthetic
classif accdg to composition
exact composition of culture media is known
synthetic
classif accdg to composition
exact composition of culture media is not known
non-synthetic
classif accdg to form or distribution
plated
tubed
