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Botany > Bacte Lab - Quiz 1 > Flashcards

Bacte Lab - Quiz 1 Flashcards

1
Q

– materials containing all essential nutrients for bacterial growth

  • → for bacterial growth
  • culture media may be in granular or powdered form
A

Culture media

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2
Q

-food and soil (where it is planted) for bacteria

A

Culture media

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3
Q

for how long and at what temperature must the CM have for the bacteria to grow

A

35 – 37C, 18 – 24 hours

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4
Q

2 forms of CM

A

granular or powdered form

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5
Q

CM for motility

A

Sulfide-Indole-Motility (SIM)

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6
Q

Characteristics of Good Culture Media

A
  1. Sterile
  2. Free of inhibitory substances
  3. Adequate amount of Salt, Water & Certain Essential Nutrients
  4. Has a proper pH
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7
Q

Classif of CM accdg to physical state or consistency

A

solid
semi-solid
liquid

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8
Q

Classif of CM accdg to physical state or consistency

no solidifying agent

A

liquid

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9
Q

Classif of CM accdg to physical state or consistency

routinely used for bacterial cultivation

A

liquid

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10
Q

sign of bacterial growth in liquid media

A

turbidity

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11
Q

example of liquid media

A

broth

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12
Q

Classif of CM accdg to physical state or consistency

with solidifying agent

A

solid

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13
Q

most common solidifying agent

A

agar

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14
Q

examples of solidifying agents

A

agar
albumin
gelatin

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15
Q

solidifying agent that is used as a diagnostic medium

A

*Gelatin

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16
Q

agar is liquid at what temp

A

100C

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17
Q

agar is solid at what temp

A

38C

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18
Q

Gelatin is solid at what temp

A

25C

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19
Q

agar concentration of solid media

A

1.5-3%

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20
Q

agar conc of semi-solid media

A

0.5 - 1%

21
Q

Classif of CM accdg to physical state or consistency

used to determine if bacteria is motile or not

A

semi-solid

22
Q

classif based on application or function

A 
basal/ordinary/simple
enriched
selective
differential
assay medium
maintenance w/o glucose
23
Q

classif based on application or function

useful for assaying vitamins, antibiotics, amino acids

A

assay medium

24
Q

classif based on application or function

with inhibitory substances

A

selective

25
Q

classif based on application or function

differentiates types of bacteria using dye or indicator

A

differential

26
Q

classif based on application or function
supports growth of fastidious bacteria
enriching substances are added

A

enriched

27
Q

classif based on application or function

basic medium from which you can prepare different media

A

Simple/Basal/Ordinary

28
Q

classif based on application or function

supports the growth of non-fastidious bacteria

A

Simple/Basal/Ordinary

29
Q

bacteria that grows immediately in a nutrient agar without added enriching substances

A

non-fastidious bacteria

30
Q

enriching substances added:

A
  • blood
  • serum
  • acidic fluid
31
Q

examples of enriched media (3)

A
  • Loeffler’s serum agar
  • Chocolate Agar – contains RBCs
  • Blood Agar
32
Q

ex of differential media

A

EMB
MacConkey
Mannitol-Salt agar

33
Q

indicator of Eosin-methylene blue agar

A

eosin-methylene blue

34
Q

differential media used for for Escherichia coli (greenish metallic sheen)

A

EMB

35
Q

differentiates lactose fermenters from non-lactose fermenters

A

EMB

36
Q

colors of lactose fermenters

A

purple, red, pink

37
Q

colors of non-lactose fermenters

A

white, colorless, yellow

38
Q

indicator of MacConkey

A

neutral red

39
Q

indicator of Mannitol-Salt agar

A

phenol red

40
Q

differential media for growth of Staphylococcus aureus

-

A

Mannitol-Salt agar

41
Q

differentiates Staphylococcus aureus from Staphylococcus epidermidis

A

Mannitol-Salt agar

42
Q

selective media that inhibits growth of Gram (+):

A
  • Gentian violet
  • Na deoxycholate
  • Bile salt
43
Q

selective media that Inhibits growth of Gram (-)

A
  • Na azide

* K tellurite

44
Q

selective media which Prevents spreading of Proteus

A
  • Chloral hydrate

* Alcohol

45
Q

classif accdg to composition

A

synthetic

non-synthetic

46
Q

classif accdg to composition

exact composition of culture media is known

A

synthetic

47
Q

classif accdg to composition

exact composition of culture media is not known

A

non-synthetic

48
Q

classif accdg to form or distribution

A

plated

tubed

Botany (324 decks)

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