BACTE LEC - Nonfermentative Gram Negative

Question 1

Oxidatively metabolize carbohydrates

Answer 1

Nonfermentative gram negative bacteria

Question 2

Forms very weak acids

Answer 2

Nonfermentative gram negative bacteria

Question 3

Nonsaccharolytic or nonoxidizers

Answer 3

Nonfermentative gram negative bacteria

Question 4

Cannot utilize carbohydrates in the presence or absence of oxygen

Answer 4

Nonsaccharolytic or nonoxidizers

Question 5

Rely on compounds other than carbohydrates

Answer 5

Nonsaccharolytic or nonoxidizers

Question 6

spore forming activity of Nonfermentative gram negative bacteria

Answer 6

Nonsporeformers

Question 7

are Nonfermentative gram negative bacteria aerobes, anaerobes, facultative, anaerobe, etc?

Answer 7

obligate aerobes

Question 8

TSI Nonfermentative gram negative

Answer 8

K/K

Question 9

indicator of contamination in hospitals

Answer 9

Nonfermentative gram negative bacteria

Question 10

Nonfermentative gram negative bacteria are usual contaminants of ??? [4]

Answer 10

water, respiratory care equipment, water baths, sinks

Question 11

Nonfermentative gram negative bacteria are what kind of pathogens

Answer 11

opportunistic

Question 12

Nonfermentative gram negative bacteria grow better on ___ than on ___

Answer 12

grow better on BA than MAC

Question 13

appearance of Nonfermentative gram negative bacteria on BA

Answer 13

dull gray colonies

Question 14

Specific O-F Medium

Answer 14

Hugh and Leifson Medium

Question 15

Carb concentration of O-F Medium

Answer 15

high at 1%

Question 16

why high Carb concentration of O-F Medium

Answer 16

to enhance the production of acidic end products

Question 17

peptone concentration of O-F Medium

Answer 17

low at 0.2%

Question 18

why low peptone concentration of O-F Medium

Answer 18

to decrease the formation of oxidative products

Question 19

what happens if there is a high peptone concentration

Answer 19

acid production will be high and is false + for fermentation

Question 20

sugars in OF medium

Answer 20

glucose, maltose, xylose

Question 21

indicator in OF medium

Answer 21

bromthymol blue

Question 22

ph range of bromthymol blue

Answer 22

ph 6-9

Question 23

positive result of OF medium

Answer 23

yellow in acidic pH

Question 24

negative result of OF medium

Answer 24

blue in alkaline ph

Question 25

reaction of oxidative organism in CT

Answer 25

Green or blue green

Question 26

reaction of oxidative organism in OT

Answer 26

yellow

Question 27

can only use carb when O2 is present

Answer 27

oxidative organism

Question 28

reaction of fermentative organism in OT & CT

Answer 28

both yellow

Question 29

common activity of facultative anaerobe

Answer 29

fermentative

Question 30

aerobe but can survive w/o O2

Answer 30

Facultative anaerobe

Question 31

purpose of zinc

Answer 31

for stability of result (?)

Question 32

reduction of nitrates, meaning of red color

Answer 32

negative rxn [presence of NO3]

Question 33

in reduction of nitrates, meaning of no change

Answer 33

No change indicates complete reduction of NO3
to N2 (positive reaction)

Question 34

in modified indol test, indole is extracted by

Answer 34

xylene

Question 35

reagent in modified indol test

Answer 35

PDAB [paradiethylaminobenzaldehyde]