BACTE LEC - Nonfermentative Gram Negative Flashcards
Oxidatively metabolize carbohydrates
Nonfermentative gram negative bacteria
Forms very weak acids
Nonfermentative gram negative bacteria
Nonsaccharolytic or nonoxidizers
Nonfermentative gram negative bacteria
Cannot utilize carbohydrates in the presence or absence of oxygen
Nonsaccharolytic or nonoxidizers
Rely on compounds other than carbohydrates
Nonsaccharolytic or nonoxidizers
spore forming activity of Nonfermentative gram negative bacteria
Nonsporeformers
are Nonfermentative gram negative bacteria aerobes, anaerobes, facultative, anaerobe, etc?
obligate aerobes
TSI Nonfermentative gram negative
K/K
indicator of contamination in hospitals
Nonfermentative gram negative bacteria
Nonfermentative gram negative bacteria are usual contaminants of ??? [4]
water, respiratory care equipment, water baths, sinks
Nonfermentative gram negative bacteria are what kind of pathogens
opportunistic
Nonfermentative gram negative bacteria grow better on ___ than on ___
grow better on BA than MAC
appearance of Nonfermentative gram negative bacteria on BA
dull gray colonies
Specific O-F Medium
Hugh and Leifson Medium
Carb concentration of O-F Medium
high at 1%
why high Carb concentration of O-F Medium
to enhance the production of acidic end products
peptone concentration of O-F Medium
low at 0.2%
why low peptone concentration of O-F Medium
to decrease the formation of oxidative products
what happens if there is a high peptone concentration
acid production will be high and is false + for fermentation
sugars in OF medium
glucose, maltose, xylose
indicator in OF medium
bromthymol blue
ph range of bromthymol blue
ph 6-9
positive result of OF medium
yellow in acidic pH
negative result of OF medium
blue in alkaline ph
reaction of oxidative organism in CT
Green or blue green
reaction of oxidative organism in OT
yellow
can only use carb when O2 is present
oxidative organism
reaction of fermentative organism in OT & CT
both yellow
common activity of facultative anaerobe
fermentative
aerobe but can survive w/o O2
Facultative anaerobe
purpose of zinc
for stability of result (?)
reduction of nitrates, meaning of red color
negative rxn [presence of NO3]
in reduction of nitrates, meaning of no change
No change indicates complete reduction of NO3 to N2 (positive reaction)
in modified indol test, indole is extracted by
xylene
reagent in modified indol test
PDAB [paradiethylaminobenzaldehyde]
