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Botany > BACTE LEC - Misc Gram-negative Bacteria > Flashcards

BACTE LEC - Misc Gram-negative Bacteria Flashcards

1
Q

Nonmotile

Pleomorphic coccobacilli or rods [2]

A

Pasteurella multocida

Haemophilus

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2
Q

require blood for growth

A

haemophilus

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3
Q

type of factor
provided by blood agar
hematin or hemin
Unknown

A

X factor

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4
Q

type of factor
provided by NAD or coenzyme 1
Vitamin

A

V factor

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5
Q

species of haemophilus w/c require only V factor

A

H. ducreyi*
H. parainfluenza
H. paraphrohaimolyticus
H. paraphrophilus

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6
Q

not adequate to isolate Haemophilus bc it releases NADase

A

Sheep blood agar

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7
Q

inactivates NAD, no NAD, no V factor

A

NADase (by sheep blood agar)

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8
Q

culture medium w/o NADase

A

horse or rabbit blood agar

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9
Q

type of agar

may release hematin and inactivate NADase

A

choc agar

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10
Q

method for the primary isolation of Haemophilus

A

staph streak

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11
Q

in staph streak method, growth near staph is __ [describe]

A

luxuriant

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12
Q

in staph streak, what organism provided nutrients for haemophiilus growth

A

staph

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13
Q

type of hemolysis by s. aureus that releases hematin

A

beta

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14
Q

phenomenon in which there are tiny colonies around staph colony

A

satellite phenomenon

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15
Q

pathogenic spp of Haemophilus

common isolate in CSF if there is meningitis esp among children

A

H. influenzae

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16
Q

VF of H. influenzae

A

Capsule
IgA protease
Adherence mechanism
Outer membrane components

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17
Q

vaccine for influenzae

A

Hib vaccine

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18
Q

clinical manifestations of H. influenzae [3]

A

meningitis
epiglottitis
bacterial tracheitis

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19
Q

Causative agent of acute contagious conjunctivitis

A

H. aegyptius

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20
Q

other name of acute contagious conjunctivitis

A

pinkeye/sore eye

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21
Q

also called Koch-Weeks Bacillus

A

H. aegyptius

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22
Q

general MoT of haemophilus spp except for

A

inhalation

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23
Q

MoT of ducreyi

A

sexual contact

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24
Q

CA of Brazilian purpuric fever (BPF)

A

H. influenzae biogroup aegyptius

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25
Q

causes chancroid or soft chancre

A

H. ducreyi

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26
Q

causes transmitted genital ulcer disease

A

H. ducreyi

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27
Q

causes suppurative, enlarged, draining, inguinal lymph nodes

A

H. ducreyi

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28
Q

sx for H. influenzae

A

blood, CSF, from upper and lower RT,

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29
Q

sx for ducreyi

A

vaginal swab

abscess drainage

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30
Q

sx for aegyptius

A

swab for conjunctivae

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31
Q

atmospheric requirements of haemophilus

A

5-10% CO2 - Capnophilic

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32
Q

microscopic appearance of ducreyi

A

shoal, school of fish

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33
Q

shoal of fish

loosely coiled clusters of organisms lined up in parallel or appearing as fingerprints

A

H. ducreyi

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34
Q

Beta hemolytic on SBA

Clumpy nonhomogenous appearance

A

ducreyi

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35
Q

MAC appearance: larger and more mucoid

A

ducreyi

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36
Q

CAP: translucent, tannish, moist, smooth convex

w/ mousy or bleachlike odor

A

ducreyi

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37
Q

odor of ducreyi on CAP

A

mousy or bleachlike

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38
Q

most misc gram (-) bacilli are __ [atmospheric requirement and location]

A

obligate intracellular

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39
Q

faintly staining coccobacilli

small grayish colonies on GCBA

A

Francisella

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40
Q

needs agglutination test and dfat for confirming its spp

A

Francisella

41
Q

BSL number/level for francisella

A

3

42
Q

Zoonotic [2]

A

> Brucella

>Francisella

43
Q

Bioterrorism agent [2]

A

> Brucella

>Francisella

44
Q

Serological Tests for Spp ID

[2]

A

> Brucella

>Francisella

45
Q

CA of tularemia

potential agent of bioterrorism

A

F. tularensis

46
Q

zoonotic infection: rabbits

A

F. tularensis

47
Q

intracellular bacteria which resists phagocytosis

A

F. tularensis

48
Q 
pathology:
skin ulcers at infection site
lymph node infection [ulceroglandular]
lungs [pneumonic]
GI system [typhoidal]
A

F. tularensis

49
Q

severe pathology by f. tularensis

A

pneumonic infection

50
Q

causes diarrhea similar to Salmonellosis

A

F. tularensis

51
Q

Causes rabbit fever

A

F. tularensis

52
Q

diagnosis for F. tularensis

A

DFAT

agglutination testing

53
Q

grows on BCYE and Thayer-Martin w/ 10% CO2

Slow grower [3-4 weeks]

A

Brucella

54
Q

zoonotic: infects cows and cattles

A

Brucella

55
Q

specimen for DX of brucella

A

BM, Blood

56
Q

spp of brucella closely related with abortion among cows and cattles

A

B. abortus

57
Q

diagnosis of brucella

A

cultures

58
Q

spp of id of brucella

A

serological test= phage and dye sensitivty

59
Q

requires phage and dye sensitivty

A

brucella

60
Q

causative agent of whooping cough

A

B. pertussis

61
Q

faintly staining gram(-) coccobacilli

A

francisella

bordetella

62
Q

mercury droplet colonies

A

b. pertussis

63
Q

culture media for b. pertussis

A

bordet gengou agar

regan-lowe [charcoal horse blod agar]

64
Q

secondary stain is replaced with stronger dye since this organism is faintly staining

A

b. pertussis

65
Q

Example of stronger dye

A

carbolfuchsin

66
Q

3 stages of pathology of b. pertussis whooping cough

A

catarrhal
paroxysmal
convalescent

67
Q

3 stages of pathology of b. pertussis whooping cough
very mild inflammation
small mucus secretions

A

catarrhal

68
Q

3 stages of pathology of b. pertussis whooping cough
intermittent fever
body malaise
fullblown infection

A

paroxysmal

69
Q

3 stages of pathology of b. pertussis whooping cough

recovery stage

A

convalescent

70
Q

causes mild respiratory infections in human

weaker strain of bordetella

A

b. parapertussis

71
Q

causes cough in dogs [bordetella]

A

b. bronchiseptica

72
Q

grows well in nonselective agars but not MAC

A

P. multocida

73
Q

very suscep to penicillin easy to treat

A

P. multocida

74
Q

CA of cellulitis w/c may progress to osteomyelitis, joint infection, pneumonia

A

P. multocida

75
Q

thin poorly stained Gram - bacilli

A

Legionella

76
Q

requires L-cysteine for growth

A

Legionella

77
Q

legionella requires ___ for growth

A

L-cysteine

78
Q

CM for Legionella

A

BCYE

79
Q

Does Legionella grow on SBA

A

No

80
Q

CAP appearance of Legionella

A

tiny colonies

81
Q

is Legionella sac or nonsac

A

assacharolytic

82
Q

most biochem tests are negative

A

Legionella

83
Q

not easily identified by routine tests

A

Legionella

84
Q

1sr discovered in 1976, American Legion in Philadelphphia

A

Legionella

85
Q

what causes Legionellosis, Legionnaire’s Disease, Pontiac Fever

A

L. pneumophila serogroup 1

86
Q 
specimen for \_\_\_
secretion from lower RT 
cough swab
bronchial swab
expectorated sputum
cough plate
A

L. pneumophila serogroup 1

87
Q

unique Dx test for Legionella pneumophila

A

urine antigen test

88
Q

other Dx test for Legionella pneumophila [3]

A

urine antigen test
DFAT
Nucleic acid probes

89
Q

very small Gram - variable coccobacilli

A

Garnerella vaginalis

90
Q

how to differentiate G. vaginalis from Lactobacillus

A

Lactobacillus is large Gram + bacilli

91
Q

2 similar spp recovered in vaginal area or secretions

A

Lactobacillus

G. vaginalis

92
Q

associated with bacterial vaginosis, pelvic inflammatory disease [PID], postpartum sepsis

A

G. vaginalis

93
Q

presence of __ is suggestive of BV due to G. vaginalis

A

clue cells

94
Q

squamous epithelial cells with intracellular G. vaginalis

A

clue cells

95
Q

2 possible disease if there is lower abdominal pain

A

UTI

Vaginalis

96
Q

Dx for G. vaginalis

A

Amsel and Nugent scoring system

97
Q

____ are too sensitive, not ideal for ideal for diagnosing Bacterial vaginosis by G. vaginalis

A

cultures

98
Q

used for reading the pap smear [for possible infections by G. vaginalis]

A

Amsel and Nugent scoring system

99
Q

____ do not meet criteria for BV by G. vaginalis

A

Cultures

Botany (324 decks)

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