BACTE LEC - Misc Gram-negative Bacteria Flashcards
Nonmotile
Pleomorphic coccobacilli or rods [2]
Pasteurella multocida
Haemophilus
require blood for growth
haemophilus
type of factor
provided by blood agar
hematin or hemin
Unknown
X factor
type of factor
provided by NAD or coenzyme 1
Vitamin
V factor
species of haemophilus w/c require only V factor
H. ducreyi*
H. parainfluenza
H. paraphrohaimolyticus
H. paraphrophilus
not adequate to isolate Haemophilus bc it releases NADase
Sheep blood agar
inactivates NAD, no NAD, no V factor
NADase (by sheep blood agar)
culture medium w/o NADase
horse or rabbit blood agar
type of agar
may release hematin and inactivate NADase
choc agar
method for the primary isolation of Haemophilus
staph streak
in staph streak method, growth near staph is __ [describe]
luxuriant
in staph streak, what organism provided nutrients for haemophiilus growth
staph
type of hemolysis by s. aureus that releases hematin
beta
phenomenon in which there are tiny colonies around staph colony
satellite phenomenon
pathogenic spp of Haemophilus
common isolate in CSF if there is meningitis esp among children
H. influenzae
VF of H. influenzae
Capsule
IgA protease
Adherence mechanism
Outer membrane components
vaccine for influenzae
Hib vaccine
clinical manifestations of H. influenzae [3]
meningitis
epiglottitis
bacterial tracheitis
Causative agent of acute contagious conjunctivitis
H. aegyptius
other name of acute contagious conjunctivitis
pinkeye/sore eye
also called Koch-Weeks Bacillus
H. aegyptius
general MoT of haemophilus spp except for
inhalation
MoT of ducreyi
sexual contact
CA of Brazilian purpuric fever (BPF)
H. influenzae biogroup aegyptius
causes chancroid or soft chancre
H. ducreyi
causes transmitted genital ulcer disease
H. ducreyi
causes suppurative, enlarged, draining, inguinal lymph nodes
H. ducreyi
sx for H. influenzae
blood, CSF, from upper and lower RT,
sx for ducreyi
vaginal swab
abscess drainage
sx for aegyptius
swab for conjunctivae
atmospheric requirements of haemophilus
5-10% CO2 - Capnophilic
microscopic appearance of ducreyi
shoal, school of fish
shoal of fish
loosely coiled clusters of organisms lined up in parallel or appearing as fingerprints
H. ducreyi
Beta hemolytic on SBA
Clumpy nonhomogenous appearance
ducreyi
MAC appearance: larger and more mucoid
ducreyi
CAP: translucent, tannish, moist, smooth convex
w/ mousy or bleachlike odor
ducreyi
odor of ducreyi on CAP
mousy or bleachlike
most misc gram (-) bacilli are __ [atmospheric requirement and location]
obligate intracellular
faintly staining coccobacilli
small grayish colonies on GCBA
Francisella
needs agglutination test and dfat for confirming its spp
Francisella
BSL number/level for francisella
3
Zoonotic [2]
> Brucella
>Francisella
Bioterrorism agent [2]
> Brucella
>Francisella
Serological Tests for Spp ID
[2]
> Brucella
>Francisella
CA of tularemia
potential agent of bioterrorism
F. tularensis
zoonotic infection: rabbits
F. tularensis
intracellular bacteria which resists phagocytosis
F. tularensis
pathology: skin ulcers at infection site lymph node infection [ulceroglandular] lungs [pneumonic] GI system [typhoidal]
F. tularensis
severe pathology by f. tularensis
pneumonic infection
causes diarrhea similar to Salmonellosis
F. tularensis
Causes rabbit fever
F. tularensis
diagnosis for F. tularensis
DFAT
agglutination testing
grows on BCYE and Thayer-Martin w/ 10% CO2
Slow grower [3-4 weeks]
Brucella
zoonotic: infects cows and cattles
Brucella
specimen for DX of brucella
BM, Blood
spp of brucella closely related with abortion among cows and cattles
B. abortus
diagnosis of brucella
cultures
spp of id of brucella
serological test= phage and dye sensitivty
requires phage and dye sensitivty
brucella
causative agent of whooping cough
B. pertussis
faintly staining gram(-) coccobacilli
francisella
bordetella
mercury droplet colonies
b. pertussis
culture media for b. pertussis
bordet gengou agar
regan-lowe [charcoal horse blod agar]
secondary stain is replaced with stronger dye since this organism is faintly staining
b. pertussis
Example of stronger dye
carbolfuchsin
3 stages of pathology of b. pertussis whooping cough
catarrhal
paroxysmal
convalescent
3 stages of pathology of b. pertussis whooping cough
very mild inflammation
small mucus secretions
catarrhal
3 stages of pathology of b. pertussis whooping cough
intermittent fever
body malaise
fullblown infection
paroxysmal
3 stages of pathology of b. pertussis whooping cough
recovery stage
convalescent
causes mild respiratory infections in human
weaker strain of bordetella
b. parapertussis
causes cough in dogs [bordetella]
b. bronchiseptica
grows well in nonselective agars but not MAC
P. multocida
very suscep to penicillin easy to treat
P. multocida
CA of cellulitis w/c may progress to osteomyelitis, joint infection, pneumonia
P. multocida
thin poorly stained Gram - bacilli
Legionella
requires L-cysteine for growth
Legionella
legionella requires ___ for growth
L-cysteine
CM for Legionella
BCYE
Does Legionella grow on SBA
No
CAP appearance of Legionella
tiny colonies
is Legionella sac or nonsac
assacharolytic
most biochem tests are negative
Legionella
not easily identified by routine tests
Legionella
1sr discovered in 1976, American Legion in Philadelphphia
Legionella
what causes Legionellosis, Legionnaire’s Disease, Pontiac Fever
L. pneumophila serogroup 1
specimen for \_\_\_ secretion from lower RT cough swab bronchial swab expectorated sputum cough plate
L. pneumophila serogroup 1
unique Dx test for Legionella pneumophila
urine antigen test
other Dx test for Legionella pneumophila [3]
urine antigen test
DFAT
Nucleic acid probes
very small Gram - variable coccobacilli
Garnerella vaginalis
how to differentiate G. vaginalis from Lactobacillus
Lactobacillus is large Gram + bacilli
2 similar spp recovered in vaginal area or secretions
Lactobacillus
G. vaginalis
associated with bacterial vaginosis, pelvic inflammatory disease [PID], postpartum sepsis
G. vaginalis
presence of __ is suggestive of BV due to G. vaginalis
clue cells
squamous epithelial cells with intracellular G. vaginalis
clue cells
2 possible disease if there is lower abdominal pain
UTI
Vaginalis
Dx for G. vaginalis
Amsel and Nugent scoring system
____ are too sensitive, not ideal for ideal for diagnosing Bacterial vaginosis by G. vaginalis
cultures
used for reading the pap smear [for possible infections by G. vaginalis]
Amsel and Nugent scoring system
____ do not meet criteria for BV by G. vaginalis
Cultures
