BACTE LAB - DOTS/Lab

Question 1

form the foundation of National Tuberculosis Control Program

one of the first points of contact for TB suspects

Answer 1

Laboratories

Question 2

internationall recommended strategy for TB control by WHO and IUATLD

Answer 2

DOTS

Question 3

IUATLD meaning

Answer 3

International Union Against Tuberculosis and Lung Diseases

Question 4

goal of Stop TB Strategy

Answer 4

Reduce global burden of TB by 2015 by ensuring all TB patients benefit from universal access to high-quality diagnosis and patient-centered treatment

Question 5

Six Point Stop TB Strategy

Answer 5

Pursue quality DOTS expansion and enhancement
Address TB/HIV and MDR-TB
Contribute to health system strengthening
Engage all care providers
Empower patients and communities
Enable and promote research

Question 6

Main technique for diagnosis of TB utilized by DOTS program

Answer 6

DSSM

Question 7

DSSM meaning

Answer 7

Direct Sputum Smear Microscopy

Question 8

Primary sources of infection in communities

Answer 8

Smear-positive cases

Question 9

These smear cases are usually sick and seek treatment

Answer 9

smear positive pulmonary TB

Question 10

Criteria in monitoring that are not specific

Answer 10

Clinical and radiologic

Question 11

it seldom possible to diagnose relapse by means of ___

Answer 11

chest xray

Question 12

3 Things Monitored by Bacteriological Methods

Answer 12

Evolution of disease during treatment
declaration of cure or failure
relapse

Question 13

for follow-up, this procedure is more sensitive and reliable than dssm

Answer 13

culture

Question 14

why is culture more sensitive and reliable than DSSM for follow-up

Answer 14

culture will detect only living bacilli that form colonies

Microscopy (DSSM) will show all AFB, alive or dead

Question 15

Declaration of failure on smear evidence is more accurate when based on _____

Answer 15

REPEATED positive smears

Question 16

Reliable diagnosis of relapsing cases

Answer 16

Smear diagnosis

Question 17

Why culture cannot easily replace smears for follow-up

Answer 17

M. tuberculosis grows very slowly

Repeating a smear examination after 2 weeks will yield an answer before a culture could

Question 18

These reduces the spread of TB

Answer 18

detection and treatment of infectious cases

Question 18

Greatest risk of infection for lab staff

Answer 18

Sputum collection

Question 19

Considerable risk for lab tech

Answer 19

Processing of sputum for culture and drug susceptibility testing

Question 20

—particles do not remain airborne and do not transmit TB efficiently

Answer 20

Larger

Question 21

Bacterial load smear +

Answer 21

10^6 to 10^7 bacilli per ml

Question 22

Bacterial load smear -

Answer 22

10^4 or less bacilli per ml

Question 23

Most significant predictor of infectiousness of a patient

Answer 23

Difference in bacterial load

Question 24

Risk of infection depends on (4)

Answer 24

Infectiousness of source
Environment
Duration and intensity of exposure
Susceptibility of recipient

Question 25

Mechanisms of producing droplet nuclei

Answer 25

Singing and coughing

Question 26

Where to collect sputum

Answer 26

Open area outisde environment

Question 27

Where not to collect

Answer 27

Enclosed spaces like labs toilets

Question 28

Never stand where during collection

Answer 28

In front of patient

Question 29

3 separate areas for performing tb microscopy

Answer 29

Smear preparation and staining
Performing microscopy
Record keeping and storage

Question 30

Use ____ instead of surgical masks

Answer 30

N95 or HEPA filter masks

Question 31

True or False wearing gloves can give technologists a false sense of safety

Answer 31

True

Question 32

Are gloves necessary to perform sputum microscopy

Answer 32

No. Can still perform even w/o those

Question 33

Lab coats must be tied back or front

Answer 33

Back

Question 34

Lab coats prevent Tb Infection true or falsd

Answer 34

False

Question 35

Excellent disinfectant for cleaning up sputum spills

Answer 35

Phenolic agents

Question 36

Gen disinfectant

Answer 36

Fresh household bleach diluted 1:10 with water

Question 37

Good agent for cleaning bench tops

Answer 37

70% alcohol

Question 38

T or F

N95 respirator is unneccessary if technologist uses appropriate techniques

Answer 38

True

Question 39

Wearing of gloves is encouraged in smear preparation

Answer 39

T

Question 40

Once collected, allow a sputum sx to stand undistirbed for – min to (why)

Answer 40

At least 20 min to settle any aerosols

Question 41

How to remove residual sputum on wire loops

Answer 41

Insert or dip wire loop in alcohol sand washed bottle then flame