BB Lec - Current Trends pt 2 Flashcards
There are three commercial systems approved by the FDA for screening platelets for bacterial contamination:
BacT/ALERT (bioMérieux),
eBDS (Pall Corp.),
Scan- system (Hemosystem)
Measures bacteria by detecting a change in carbon- dioxide levels associated with bacterial growth
BacT/ALERT
This system (for screening platelets for bacterial contamination) provides continuous monitoring of the platelet sample–containing culture bottles, which are held for the shelf-life of the platelet unit or until a positive reaction is detected.
BacT/ALERT
culture-based systems
BacT/ALERT and eBDS
As the level of bacteria in the platelets at the time of collection can be low, samples are not taken until after at least how many hours of storage
24
measures the oxygen content of the air within the sample pouch following incubation for 18 to 30 hours.
eBDS system
in the eBDS system a decrease in oxygen level indicates the __
presence of bacteria
, is a laser-based, scanning cytometry method.
Scansystem
the first rapid test to detect bacteria in WBD platelets
Pan Genera Detection (PGD) test (Verax Biomedical)
an immunoassay that detects lipoteichoic acids on gram- positive bacteria and lipopolysaccharides on gram-negative bacteria. Both aerobes and anaerobes are detected.
Pan Genera Detection (PGD) test (Verax Biomedical)
are pooled ABO-matched, leukoreduced WBD platelets that have been cultured and are ready for transfusion.
Acrodose platelets
Acrodose platelets are produced in a closed system, they can be stored for how many days from collection
5
They provide a therapeutic dose equivalent to apheresis platelets and at a lower cost, but they do expose the recipient to multiple donors.
Acrodose platelets
Problem with 7 day storage of PLT
Septic transfusion rxns
Problem with 7 day storage of PLT
Septic transfusion rxns
