CC Lab - Glucose Flashcards
measures blood glucose exactly 2 hours after you start eating a meal
Two-hour Post Prandial Blood Sugar
how many carbs (in g) must be eaten for the Two-hour Post Prandial Blood Sugar
at least 100g
glycosylated hemoglobin
HbA1C
Other names of gluc
dextrose and blood sugar
possible tests requested by physician
FBS RBS OGTT, OGCT 2 PPBS HbA1c
test for pregnant women/with gestational diabetes
OGTT/OGCT
test which requires NPO for 6-8 hrs
FBS
Test for monitoring DM
HbA1C
HbA1C test is done every ___
3 months
excess glucose is stored in __
liver
excess glucose may be stored as (2)
glycogen
adipose tissue
hormones that regulate gluc levels
insulin and glucagon
a hyperglycemic agent
glucagon
primary hypoglycemic agent
insulin
methods for glucose determination (3)
reduction method
schiff base reaction with orthotoluidine
enzymatic method
experiment proper (name??)
enzymatic colorimetric glucose testing
most important carbohydrate
glucose
CC which needs EDTA tube
HbA1c
the rest of CC tests need (Color of top)
red or yellow top
Principle in glucose testing (?)
glucose + oxygen + water ——> gluconic acid + H2O2
see notebook
insulin is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis
gluconeogenesis
glucagon is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis
glycogenolysis
3 methods for glucose determination
reduction method
schiff base reaction with orthotoluidine
3 methods for glucose determination
alkaline method
based on the ability of glucose to reduce copper or ferricyanide
reduction method
type of reduction method which includes Nelson-Somogyi, Folin-Wu, Iodometric
Copper reduction
type of reduction method
inverse colorimetry
Ferricyanide method
principle in ferricyanide method
ferricyanide (yellow) is converted to ferrocyanide (colorless)
3 methods for glucose determination
in a hot acidic medium or glacial acetic acid
schiff base reaction with orthotoluidine
Purpose of glucose measurement
Determine capacity of body to maintain normal level of glucose
Purpose of glucose measurement
Determine capacity of body to maintain normal level of glucose
glucose measurement significant in diagnosing what disease
Diabetes mellitus
Indicator in the glucose measurement
Quinoneimine dye
Color of quinoneimine dye
Red violet
Assay requirements
Wavelength - 500 nm
Optical path - 1 cm
Temperature - 20-25C
Measurement - against reagent blank
Only — reagent blank used per series
1
How long to incubate and at what temp
10 min
20-25
How much standard solution must be placed
0.01 ml
How much control normal serum, control pathologic serum, patient’s serum and standard solution must be placed
0.01 ml
Total volume in the cuvets
1.01 ml except for the blank which is 1.0 ml only
Pipetting scheme
Semi-micro
