CC Lab - Glucose Flashcards

1
Q

measures blood glucose exactly 2 hours after you start eating a meal

A

Two-hour Post Prandial Blood Sugar

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2
Q

how many carbs (in g) must be eaten for the Two-hour Post Prandial Blood Sugar

A

at least 100g

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3
Q

glycosylated hemoglobin

A

HbA1C

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4
Q

Other names of gluc

A

dextrose and blood sugar

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5
Q

possible tests requested by physician

A
FBS 
RBS
OGTT, OGCT
2 PPBS
HbA1c
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6
Q

test for pregnant women/with gestational diabetes

A

OGTT/OGCT

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7
Q

test which requires NPO for 6-8 hrs

A

FBS

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8
Q

Test for monitoring DM

A

HbA1C

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9
Q

HbA1C test is done every ___

A

3 months

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10
Q

excess glucose is stored in __

A

liver

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11
Q

excess glucose may be stored as (2)

A

glycogen

adipose tissue

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12
Q

hormones that regulate gluc levels

A

insulin and glucagon

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13
Q

a hyperglycemic agent

A

glucagon

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14
Q

primary hypoglycemic agent

A

insulin

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15
Q

methods for glucose determination (3)

A

reduction method
schiff base reaction with orthotoluidine
enzymatic method

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16
Q

experiment proper (name??)

A

enzymatic colorimetric glucose testing

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17
Q

most important carbohydrate

A

glucose

18
Q

CC which needs EDTA tube

A

HbA1c

19
Q

the rest of CC tests need (Color of top)

A

red or yellow top

20
Q

Principle in glucose testing (?)

A

glucose + oxygen + water ——> gluconic acid + H2O2

see notebook

21
Q

insulin is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

A

gluconeogenesis

22
Q

glucagon is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

A

glycogenolysis

23
Q

3 methods for glucose determination

A

reduction method

schiff base reaction with orthotoluidine

24
Q

3 methods for glucose determination
alkaline method
based on the ability of glucose to reduce copper or ferricyanide

A

reduction method

25
Q

type of reduction method which includes Nelson-Somogyi, Folin-Wu, Iodometric

A

Copper reduction

26
Q

type of reduction method

inverse colorimetry

A

Ferricyanide method

27
Q

principle in ferricyanide method

A

ferricyanide (yellow) is converted to ferrocyanide (colorless)

28
Q

3 methods for glucose determination

in a hot acidic medium or glacial acetic acid

A

schiff base reaction with orthotoluidine

29
Q

Purpose of glucose measurement

A

Determine capacity of body to maintain normal level of glucose

29
Q

Purpose of glucose measurement

A

Determine capacity of body to maintain normal level of glucose

30
Q

glucose measurement significant in diagnosing what disease

A

Diabetes mellitus

31
Q

Indicator in the glucose measurement

A

Quinoneimine dye

32
Q

Color of quinoneimine dye

A

Red violet

33
Q

Assay requirements

A

Wavelength - 500 nm
Optical path - 1 cm
Temperature - 20-25C
Measurement - against reagent blank

34
Q

Only — reagent blank used per series

A

1

35
Q

How long to incubate and at what temp

A

10 min

20-25

36
Q

How much standard solution must be placed

A

0.01 ml

37
Q

How much control normal serum, control pathologic serum, patient’s serum and standard solution must be placed

A

0.01 ml

38
Q

Total volume in the cuvets

A

1.01 ml except for the blank which is 1.0 ml only

39
Q

Pipetting scheme

A

Semi-micro