CC Lab - Glucose

Question 1

measures blood glucose exactly 2 hours after you start eating a meal

Answer 1

Two-hour Post Prandial Blood Sugar

Question 2

how many carbs (in g) must be eaten for the Two-hour Post Prandial Blood Sugar

Answer 2

at least 100g

Question 3

glycosylated hemoglobin

Answer 3

HbA1C

Question 4

Other names of gluc

Answer 4

dextrose and blood sugar

Question 5

possible tests requested by physician

Answer 5

FBS 
RBS
OGTT, OGCT
2 PPBS
HbA1c

Question 6

test for pregnant women/with gestational diabetes

Answer 6

OGTT/OGCT

Question 7

test which requires NPO for 6-8 hrs

Answer 7

FBS

Question 8

Test for monitoring DM

Answer 8

HbA1C

Question 9

HbA1C test is done every ___

Answer 9

3 months

Question 10

excess glucose is stored in __

Answer 10

liver

Question 11

excess glucose may be stored as (2)

Answer 11

glycogen

adipose tissue

Question 12

hormones that regulate gluc levels

Answer 12

insulin and glucagon

Question 13

a hyperglycemic agent

Answer 13

glucagon

Question 14

primary hypoglycemic agent

Answer 14

insulin

Question 15

methods for glucose determination (3)

Answer 15

reduction method
schiff base reaction with orthotoluidine
enzymatic method

Question 16

experiment proper (name??)

Answer 16

enzymatic colorimetric glucose testing

Question 17

most important carbohydrate

Answer 17

glucose

Question 18

CC which needs EDTA tube

Answer 18

HbA1c

Question 19

the rest of CC tests need (Color of top)

Answer 19

red or yellow top

Question 20

Principle in glucose testing (?)

Answer 20

glucose + oxygen + water ——> gluconic acid + H2O2

see notebook

Question 21

insulin is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

Answer 21

gluconeogenesis

Question 22

glucagon is responsible for what type of glucose process (glycolysis, glycogenolysis, gluconeogenesis, glycogenesis

Answer 22

glycogenolysis

Question 23

3 methods for glucose determination

Answer 23

reduction method

schiff base reaction with orthotoluidine

Question 24

3 methods for glucose determination
alkaline method
based on the ability of glucose to reduce copper or ferricyanide

Answer 24

reduction method

Question 25

type of reduction method which includes Nelson-Somogyi, Folin-Wu, Iodometric

Answer 25

Copper reduction

Question 26

type of reduction method

inverse colorimetry

Answer 26

Ferricyanide method

Question 27

principle in ferricyanide method

Answer 27

ferricyanide (yellow) is converted to ferrocyanide (colorless)

Question 28

3 methods for glucose determination

in a hot acidic medium or glacial acetic acid

Answer 28

schiff base reaction with orthotoluidine

Question 29

Purpose of glucose measurement

Answer 29

Determine capacity of body to maintain normal level of glucose

Question 29

Purpose of glucose measurement

Answer 29

Determine capacity of body to maintain normal level of glucose

Question 30

glucose measurement significant in diagnosing what disease

Answer 30

Diabetes mellitus

Question 31

Indicator in the glucose measurement

Answer 31

Quinoneimine dye

Question 32

Color of quinoneimine dye

Answer 32

Red violet

Question 33

Assay requirements

Answer 33

Wavelength - 500 nm
Optical path - 1 cm
Temperature - 20-25C
Measurement - against reagent blank

Question 34

Only — reagent blank used per series

Answer 34

1

Question 35

How long to incubate and at what temp

Answer 35

10 min

20-25

Question 36

How much standard solution must be placed

Answer 36

0.01 ml

Question 37

How much control normal serum, control pathologic serum, patient’s serum and standard solution must be placed

Answer 37

0.01 ml

Question 38

Total volume in the cuvets

Answer 38

1.01 ml except for the blank which is 1.0 ml only

Question 39

Pipetting scheme

Answer 39

Semi-micro