MYCOVIRO - CH 60 of book part 1 Flashcards
Produce large, ribbon-like hyphae that contain occasional septa
MUCORALES (ZYGOMYCETES)
- Have sporangia (saclike fruiting structures) that produce sporangiospores and is formed at the tip of a
supporting structure called sporangiophore
MUCORALES (ZYGOMYCETES)
- Sporangiophores are connected to one another by occasionally septate hyphae called stolons, attached
to contact points where root-like structure (rhizoids) may appear and anchor the organism to the agar
surface.
MUCORALES (ZYGOMYCETES)
- Commonly found on decaying vegetable matter or old bread or in soil
MUCORALES (ZYGOMYCETES)
- infection caused by mucorales
Mucormycosis
can lead to vascular invasion and rapidly produce thrombosis and tissue necrosis - perineural invasion can also occur
Mucormycosis
one of the most common presentation of mucormycosis
- rhinocerebral form
infection w/c involves nasal mucosa, palate, sinuses, orbit, face and brain
- rhinocerebral form
stains for mucorales ID
Calcofluor white or KOH
What to observe under microscope for mucorales ID
observe branching, broad-diameter, predominantly nonseptated hyphae
Cultivation: Fluffy, white to gray or brown hyphal growth, resembles cotton candy
mucorales
grows rapidly; covers agar suface within 24 to 96 hours
mucorales
hyphae may lift the lid of agar plate (aka “lid lifter”)
mucorales
may appear to be coarse - dish is filled with loose, grayish hyphae, dotted with brown or black sporangia
mucorales
Mucorales spp
- unbranched sporangiophores; rhizoids appear opposite the point where stolon arises
Rhizopus spp
Mucorales spp
- singularly produced or branched sporangiophores with round sporangium at the tip; no
rhizoids or stolons
Mucor spp.
Mucorales spp
- pyriform sporangia with funnel-shaped area (apophysis)
- rhizoids originate between sporangiophores as with Absidia spp.
Lichtheimia spp.
- infections caused by dermatophytes involving the superficial areas of the body
Dermatomycoses
- most common fungal infections; referred to as tinea (“ringworm”)
Dermatomycoses
- they break down and utilize keratin as nitrogen source
dermatophytes
- usually incapable of penetrating the subcutaneous tissue unless host is immunocompromised (even then, it is rare)
dermatophytes
gross appearance of lesion of which fungus: outer ring of the active progressing infection, with central healing
dermatophytes
stains for dermatophytes (2)
Calcofluor white or KOH
observe what structures for dermatophytes
presence of hyaline septate hyphae and/or arthroconidia
- infected hair shaft may be seen filled with masses of large arthroconidia in chains (endothrix invasion) or may show external masses of spores that ensheath the hair shaft (ectothrix invasion)
dermatophytes/dermatomycoses
used for final ID of dermatophytes
cultivation
what fungal infection reveal hyphae and air spaces within the shaft
T. schoenleinii
initial growth of dermatophytes is often subcultured onto what agars (2) to induce sporulation
cornmeal agar or potato dextrose agar
- most common causes of feet and nails infection
TRICHOPHYTON SPP.
- most are anthrophilic (“human-loving”), few are zoophilic (primarily infecting animals)
TRICHOPHYTON SPP.