CC Lab - Spectrophotometry Flashcards
major categories of analytical techniques
spectrometry
luminescence
electroanalytical method
chromatography
main principle of spectro
beer’s law/beer-lambert’s law
3 major methods for calculation of unknown solution
direct comparison/ratio of standard to unknown
use of standard curve
use of molar absorptivity value
source of reagent for exp
reagent kit
3 substances found in reagent
catalyst
enzyme
buffer
type of spectrophotometer in the lab
Humalyzer Primus
old spectrophotometer
ALS 2000
Conventional parts of spectrophotometer (6)
exciter lamp variable slit diaphragm monochromator sample holder photodetector read out device
parts of spectrophotometer
light source
exciter lamp
parts of spectrophotometer
separates radiant energy
monochromator
parts of spectrophotometer
isolate narrow band of light
variable slit diaphragm
wavelength of glucose
500 nm
units of wavelength
nm or mu or 10^-9m
parts of spectrophotometer
holds smaple
sample holder/cuvetter
shape of cuvette
round or square
parts of spectrophotometer
reads amount of transmitted light then converts it to an electrical signal
photodetector
parts of spectrophotometer
galvanometer
converts electric signal to a readable data
read out device
keypad (4 commands)
ESC
Enter
Rinse
Paper
rinsing solution
triple distilled water
wait how many min after turning on the spectrophotometer
5 min
2 types of operating procedure
Kinetic or rate method
Endpoint method
2 types of operating procedure
Kinetic or rate method
Operating procedure for ALS 2000 Spectrophotometer
Endpoint method
No sx
To set reading at 0
Blank
Blank solution with reagent
Reagent blank
Blank soln with only the sample, no rgt
Serum or sample blank
Empty test tube to set reading at zero
Air blank
Tool for precision which BEHAVES LIKE A SPECIMEN
Controls
