HEMA LAB - Staining

Question 1

other methods of staining

placing blood smear on a rack

Answer 1

staining dish method

Question 2

automated method of staining 3 kinds

Answer 2

hemasteiner automatic slide stainer
hema-tek 1000 slide stainer
hema-tek 2000 slide stainer

Question 3

automated method

freshly prepared staining sol’n used daily or every 4-8 hours

Answer 3

hemasteiner automatic slide stainer

Question 4

automated method

bottles of stain-pak are opened by making a small hole and a cannula is inserted into each solution

Answer 4

hema-tek 1000 slide stainer

Question 5

automated method

innovation: use of new pumps and volumee controls

Answer 5

hema-tek 2000 slide stainer

Question 6

2 types of stain used in diff count

Answer 6

romanowsky stain and panoptic stain

Question 7

type of stain used in diff count
contains methylene blue or its oxidative product, azure B
also contains eosin Y or eosin B

Answer 7

romanowsky stain

Question 8

type of stain used in diff count

dyes produce multiple colors when used on cells

Answer 8

romanowsky stain

Question 9

type of stain used in diff count

polychromatic stain

Answer 9

romanowsky stain

Question 10

types of romanowsky stain [3]

Answer 10

Wright’s
Giemsa
Leishman, Jenner, May-Grunwald

Question 11

types of romanowsky stain

most satisfactory

Answer 11

wright’s stain

Question 12

types of romanowsky stain

for demo on wbc, inclusion bodies, intracellular parasites

Answer 12

giemsa

Question 13

types of romanowsky stain

similar to wright’s stain except for the method used to oxidize methylene blue

Answer 13

Leishman, Jenner, May-Grunwald

Question 14

consist of romanowsky stain and another dye

Answer 14

panoptic stain

Question 15

panoptic stains [3]

Answer 15

Wright’s-Giemsa
Jenner-Giemsa
May-Grunwald-Giemsa

Question 16

methods of smear prep [3]

Answer 16

two-slide or wedge method
two coverslip or ehrlich’s two-coverglass
spinner’s method

Question 17

spinner’s method is done using an instrument called

Answer 17

hemaspinner

Question 18

in spinner’s method, the clean glass is placed on a –

Answer 18

platen

Question 19

modern spinners contain __ to detect if cells have separated properly. this prompts the platen to automatically stop spinning

Answer 19

optical system with sensor

Question 20

instrument for automated slide-making and staining of blood

smears prep after analysis of blood sample

Answer 20

sysmex SP-1000i

Question 21

in sysmex SP-1000i smears are prepared every –

Answer 21

30 sec

Question 22

amount of blood, angle, speed of spreader in sysmex SP-1000i dependent on –

Answer 22

Hct value

Question 23

another instrument aside from sysmex SP-1000i for smear making and analyzing

Answer 23

beckman coulter blood analyzer