HEMA LAB - Staining Flashcards
other methods of staining
placing blood smear on a rack
staining dish method
automated method of staining 3 kinds
hemasteiner automatic slide stainer
hema-tek 1000 slide stainer
hema-tek 2000 slide stainer
automated method
freshly prepared staining sol’n used daily or every 4-8 hours
hemasteiner automatic slide stainer
automated method
bottles of stain-pak are opened by making a small hole and a cannula is inserted into each solution
hema-tek 1000 slide stainer
automated method
innovation: use of new pumps and volumee controls
hema-tek 2000 slide stainer
2 types of stain used in diff count
romanowsky stain and panoptic stain
type of stain used in diff count
contains methylene blue or its oxidative product, azure B
also contains eosin Y or eosin B
romanowsky stain
type of stain used in diff count
dyes produce multiple colors when used on cells
romanowsky stain
type of stain used in diff count
polychromatic stain
romanowsky stain
types of romanowsky stain [3]
Wright’s
Giemsa
Leishman, Jenner, May-Grunwald
types of romanowsky stain
most satisfactory
wright’s stain
types of romanowsky stain
for demo on wbc, inclusion bodies, intracellular parasites
giemsa
types of romanowsky stain
similar to wright’s stain except for the method used to oxidize methylene blue
Leishman, Jenner, May-Grunwald
consist of romanowsky stain and another dye
panoptic stain
panoptic stains [3]
Wright’s-Giemsa
Jenner-Giemsa
May-Grunwald-Giemsa
methods of smear prep [3]
two-slide or wedge method
two coverslip or ehrlich’s two-coverglass
spinner’s method
spinner’s method is done using an instrument called
hemaspinner
in spinner’s method, the clean glass is placed on a –
platen
modern spinners contain __ to detect if cells have separated properly. this prompts the platen to automatically stop spinning
optical system with sensor
instrument for automated slide-making and staining of blood
smears prep after analysis of blood sample
sysmex SP-1000i
in sysmex SP-1000i smears are prepared every –
30 sec
amount of blood, angle, speed of spreader in sysmex SP-1000i dependent on –
Hct value
another instrument aside from sysmex SP-1000i for smear making and analyzing
beckman coulter blood analyzer
