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Botany > Hema Lab - Cell Counting > Flashcards

Hema Lab - Cell Counting Flashcards

1
Q

2 Methods of Cell Counting

A

manual method

automation

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2
Q

2 basis/method of manual method

A

turbidimetric technique

hemacytometer

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3
Q

type of manualmethod that estimates by means of turbidimetry

A

turbidimetric technique

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4
Q

components of hemacytometer

A 
counting chamber
thoma pipette (RBC, WBC)
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5
Q

Types of Counting Chamber

A

Fuchs-Rosenthal
Improved Neubauer
Neubauer Counting Chamber
Speirs-Levy

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6
Q

pipets used for blood dilution

A

thoma pipettes

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7
Q

parts of a thoma pipette

A

stem
bead
bulb

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8
Q

distinct calibrations/lines on thoma pipette

A

0.5

1

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9
Q

highest mark on RBC Thoma pipette

A

101

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10
Q

highest mark on WBC Thoma pipette

A

11

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11
Q

bore size of RBC pipette

A

smaller

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12
Q

bore size of WBC pipette

A

larger

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13
Q

number of drops to discard on RBC pipette

A

5-6

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14
Q

number of drops to discard on WBC pipette

A

2-3

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15
Q

volume capacity of RBC pipette

A

100 units

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16
Q

volume capacity of WBC pipette

A

10 units

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17
Q

Why do we need to discard first few drops from the Thoma pipette

A

The blood is in the bulb, while the diluting fluid is in the stem.

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18
Q

purpose of bead

A

aids in mixing

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19
Q

standard dilution for RBC count

A

1:200

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20
Q

standard dilution for RBC count

A

1:20

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21
Q

type of CC

larger than Neubauer CC

A

Fuchs- Rosenthal

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22
Q

type of CC

used in low cell count:eosinohil, spinal fluid, leukopenic blood count, basophil count

A

Fuchs-Rosenthal

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23
Q

Fuchs-Rosenthal uses (4)

A 
low cell count:
eosinohil, 
spinal fluid, 
leukopenic blood count, 
basophil count
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24
Q

measurement of primary square of F-R CC

A

4x4 mm

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25
Q

depth of Fuchs-Rosenthal

A

0.2 mm

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26
Q

How many ruled areas in F-R

A

2 (upper and lower)

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27
Q

Primary square of F-R has how many secondary squares

A

16

28
Q

Measurement of secondary squares of F-R

A

1x1 mm or 1 sq mm

29
Q

Secondary square of F-R has how many tertiarysquares

A

1/16 sq mm (1/4 x 1/4)

30
Q

Number of tertiary squares per ruled area in F-R

A

256

31
Q

Number of tertiary squares in one F-R CC

A

512

32
Q

No. of ruled areas in S-L CC

A

4 ruled areas

33
Q

number of SECONDARY squares in each ruled area in S-L CC

A

10 squares

34
Q

measurement of secondary squares in S-L CC

A

1x1 mm or 1 sq mm

35
Q

depth of S-L CC

A

0.2 MM

36
Q

T or F: All CC have a secondary square that measures 1x2 mm

A

True

37
Q

measurement of primary square in S-L CC

A

5mm x 2 mm or 10 sq mm

38
Q

number of primary squares in S-L CC

A

4 primary squares

39
Q

number of tertiary squares in each secondary square in S-L CC

A

16

40
Q

measurement of tertiary square in S-L CC

A

1/16 sq mm or 0.25 x 0.25 mm

41
Q

number of tertiary squares in one ruled area of S-L CC

A

160

42
Q

number of tertiary squares in one S-L CC

A

640

43
Q

number of secondary squares in one S-L CC

A

40

44
Q

CC used for all manual cellular counts using blood & other body fluids

A

Improved Neubauer Counting Chamber

45
Q

measurement of primary square in INCC

A

9 sq mm or 3x3 mm

46
Q

number of secondary squares in INCC

A

9

47
Q

measurement of secondary square in INCC

A

1x1 mm or 1 sq mm

48
Q

number of tertiary squares in central secondary square of INCC

A

25

49
Q

Measurement of one tertiary square in the central secondary square INCC

A

1/25 sq mm or 1/5 x 1/5

50
Q

number of smallest squares in one tertiary square in INCC

A

16

51
Q

square used in platelet count using INCC

A

entire central secondary square

52
Q

uses of central secondary square of INCC

A

platelet and RBC count

53
Q

square/s used in RBC count using INCC

A

4 corner tertiary squares in the central secondary square + the central tertiary square

54
Q

number of tertiary squares in 4 corner secondary square of INCC

A

16

55
Q

measurement of one tertiary square in 4 corner secondary square of INCC

A

1/4 mm x 1/4 mm or 1/16 sq mm

56
Q

total number of smallest squares in central secondary square of INCC

A

400

57
Q

total number of smallest squares in central secondary square used in RBC count using INCC

A

80

58
Q

similar to INCC except that the central secondary square is divided into 16 tertiary squares only

A

NCC

Neubauer Counting Chamber

59
Q

total number of smallest squares in NCC

A

16 X 16 = 256 smallest square

60
Q

total number of smallest squares in central secondary square used in RBC count USING NCC

A

80

61
Q

area counted in INCC in Standard RBC count

A

number of tertiary squares used in RBC count x area of each tertiary square
5 x 1/25 = 1/5 sq mm

62
Q

area counted in NCC in Standard RBC count

A

number of tertiary squares used in RBC count x area of each tertiary square
5 x 1/16 = 5/16 sq mm

63
Q

formula in volume calculations

A

area x depth

64
Q

depth of INCC

A

0.1 mm

65
Q

area from 0.1 to 101

A

mixing chamber or bulb of RBC pipette

66
Q

area from 0.0 to 1.0

A

stem both RBC and WBC pipette

67
Q

area from 1.0 to 11

A

mixing chamber or bulb of WBC pipette

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