Hema Lab - Cell Counting Flashcards
2 Methods of Cell Counting
manual method
automation
2 basis/method of manual method
turbidimetric technique
hemacytometer
type of manualmethod that estimates by means of turbidimetry
turbidimetric technique
components of hemacytometer
counting chamber thoma pipette (RBC, WBC)
Types of Counting Chamber
Fuchs-Rosenthal
Improved Neubauer
Neubauer Counting Chamber
Speirs-Levy
pipets used for blood dilution
thoma pipettes
parts of a thoma pipette
stem
bead
bulb
distinct calibrations/lines on thoma pipette
0.5
1
highest mark on RBC Thoma pipette
101
highest mark on WBC Thoma pipette
11
bore size of RBC pipette
smaller
bore size of WBC pipette
larger
number of drops to discard on RBC pipette
5-6
number of drops to discard on WBC pipette
2-3
volume capacity of RBC pipette
100 units
volume capacity of WBC pipette
10 units
Why do we need to discard first few drops from the Thoma pipette
The blood is in the bulb, while the diluting fluid is in the stem.
purpose of bead
aids in mixing
standard dilution for RBC count
1:200
standard dilution for RBC count
1:20
type of CC
larger than Neubauer CC
Fuchs- Rosenthal
type of CC
used in low cell count:eosinohil, spinal fluid, leukopenic blood count, basophil count
Fuchs-Rosenthal
Fuchs-Rosenthal uses (4)
low cell count: eosinohil, spinal fluid, leukopenic blood count, basophil count
measurement of primary square of F-R CC
4x4 mm
depth of Fuchs-Rosenthal
0.2 mm
How many ruled areas in F-R
2 (upper and lower)
Primary square of F-R has how many secondary squares
16
Measurement of secondary squares of F-R
1x1 mm or 1 sq mm
Secondary square of F-R has how many tertiarysquares
1/16 sq mm (1/4 x 1/4)
Number of tertiary squares per ruled area in F-R
256
Number of tertiary squares in one F-R CC
512
No. of ruled areas in S-L CC
4 ruled areas
number of SECONDARY squares in each ruled area in S-L CC
10 squares
measurement of secondary squares in S-L CC
1x1 mm or 1 sq mm
depth of S-L CC
0.2 MM
T or F: All CC have a secondary square that measures 1x2 mm
True
measurement of primary square in S-L CC
5mm x 2 mm or 10 sq mm
number of primary squares in S-L CC
4 primary squares
number of tertiary squares in each secondary square in S-L CC
16
measurement of tertiary square in S-L CC
1/16 sq mm or 0.25 x 0.25 mm
number of tertiary squares in one ruled area of S-L CC
160
number of tertiary squares in one S-L CC
640
number of secondary squares in one S-L CC
40
CC used for all manual cellular counts using blood & other body fluids
Improved Neubauer Counting Chamber
measurement of primary square in INCC
9 sq mm or 3x3 mm
number of secondary squares in INCC
9
measurement of secondary square in INCC
1x1 mm or 1 sq mm
number of tertiary squares in central secondary square of INCC
25
Measurement of one tertiary square in the central secondary square INCC
1/25 sq mm or 1/5 x 1/5
number of smallest squares in one tertiary square in INCC
16
square used in platelet count using INCC
entire central secondary square
uses of central secondary square of INCC
platelet and RBC count
square/s used in RBC count using INCC
4 corner tertiary squares in the central secondary square + the central tertiary square
number of tertiary squares in 4 corner secondary square of INCC
16
measurement of one tertiary square in 4 corner secondary square of INCC
1/4 mm x 1/4 mm or 1/16 sq mm
total number of smallest squares in central secondary square of INCC
400
total number of smallest squares in central secondary square used in RBC count using INCC
80
similar to INCC except that the central secondary square is divided into 16 tertiary squares only
NCC
Neubauer Counting Chamber
total number of smallest squares in NCC
16 X 16 = 256 smallest square
total number of smallest squares in central secondary square used in RBC count USING NCC
80
area counted in INCC in Standard RBC count
number of tertiary squares used in RBC count x area of each tertiary square
5 x 1/25 = 1/5 sq mm
area counted in NCC in Standard RBC count
number of tertiary squares used in RBC count x area of each tertiary square
5 x 1/16 = 5/16 sq mm
formula in volume calculations
area x depth
depth of INCC
0.1 mm
area from 0.1 to 101
mixing chamber or bulb of RBC pipette
area from 0.0 to 1.0
stem both RBC and WBC pipette
area from 1.0 to 11
mixing chamber or bulb of WBC pipette
