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Botany > BACTE LAB - Biochemical tests > Flashcards

BACTE LAB - Biochemical tests Flashcards

1
Q

LIA Carbohydrate

A

glucose

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2
Q

amino acid in LIA

A

Lysine

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3
Q

ph indicator

A

bromcresol purple

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4
Q

H2S indicator

A

ferric ammonium citrate

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5
Q

Lysine decarboxylase +

A

K/K purple over purple

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6
Q

Lysine decarboxylase -

A

K/A purple/yellow

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7
Q

Deaminase -

A

R/A red/yellow

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8
Q

H2S positive in LIA

A

blackening

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9
Q

indol production test medium

A

tryptone broth

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10
Q

indol production test negative result

A

yellow or brown ring

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11
Q

indol production test reagent

A

Ehrlich’s or Kovac’s (paraaminobenzaldehyde)

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12
Q

indol production test + result

A

red ring

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13
Q

methyl red test medium

A

MRVP or Methyl Red Voges Proskauer medium

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14
Q

methyl red test reagent

A

methyl red indicator

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15
Q

methyl red test + result

A

red solution

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16
Q

methyl red test - result

A

yellow solution

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17
Q

voges proskauer test medium

A

MRVP medium

18
Q

voges proskauer test reagent

A

VP Reagent A (alpha naphthol in absolute methyl alcohol)

VP Reagent B (KOH w/ creatine)

19
Q

what is produced in the voges proskauer test

20
Q

In the VP Test, acetoin is converted to?

21
Q

Citrate utilization test medium

A

simmon citrate agar

22
Q

Citrate utilization test indicator

A

bromthymol blue

23
Q

Citrate utilization test + result

A

prussian blue color

24
Q

Citrate utilization test - result

A

Green color

25
Q

urease test medium

A

urea broth

26
Q

urease test indicator

A

phenol red

27
Q

urease test positive result

A

pink to red

28
Q

urease test negative result

A

no color change

29
Q

SIM MEDIUM

A

Sulfide indol motility medium

30
Q

SIM Reagent

A

Ehrlich’s or Kovac’s

31
Q

Containers in water analysis

A

sterile glass bottles w/ round glass stoppers or screw caps

32
Q

Volume of water in water analysis

A

not less than 100 ml

33
Q

method in water analysis

A

multiple fermentation tube technique

34
Q

medium in water analysis

A

Brilliant Green Bile Lactose Broth

35
Q

tubes used in water analysis

A

durham
loeffler
hard glass

36
Q

double strength concentration

37
Q

single strength conc

38
Q

positive confirmed test result in water analysis

A

black colonies w/ metallic sheen on emb

39
Q

negative confirmed test result in water analysis

A

no black colonies w/ metallic sheen on emb

40
Q

positve completed test result

A

Gram (-) nonsporeforming bacilli
presence of turbidity
bubble/gas/space

41
Q

the results of the examinations of the replicate tubes and dilutions is expressed in terms of

A

most probable number (mpn)

Botany (324 decks)

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