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Botany > Cbc expt > Flashcards

Cbc expt Flashcards

1
Q

Universal precaution

A

Treat all body fluids infectious

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2
Q

Tests in CBC

A

Hemoglobin measurementHematocrit measurementRBC countWBC countDifferential Count

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3
Q

MEASURES Volume of packed red cell after centrifugation

A

HEMATOCRIT measurement

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4
Q

METHOD IN hematocrit measurement

A

Adam’s micromethod

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5
Q

materials in hematocrit measurement

A

capillary tube (red, blue) sealing clay/paraffin wax microhematocrit centrifuge machine microhematocrit reader

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6
Q

Procedure in hematocrit measurement

A

Fill capillary tube with blood (3/4)Seal. LabelCentrifugate. Use balancerSpin 10,000 rpm 4-5min

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7
Q

in hematocrit measurement, spin at what rpm and how many min

A

10,000 rpm 4-5min

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8
Q

Hematocrit NT

A

male- 47 +/- 7 vol% / 0.47 +/- 7 female- 42 +/- 5 vol%

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9
Q

Methods for hemoglobin measurement

A

acid-hematin method Cyanmethemoglobin method

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10
Q

Method for hemoglobin measurement that gives less error

A

Cyanmethemoglobin method

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11
Q

MATERIALS FOR hemoglobin measurement

A

0.01 N HCl Sahli’s tube (w/comparator block) Sahli’s pipette dH20 Stirring rod

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12
Q

PROCEDURE FOR hemoglobin measurement

A

Deliver 0.01N HCl into Sahli’s tube – 2markAspirate 0.02 mL bloodExpel blood into Sahli’s tubeRinse pipette with dH20-add into the tubeAdd dH20 drop by drop – comparator blockRead lower miniscus

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13
Q

NR hemoglobin measurement

A

NR: male : 14-16.5 g/100mL or 14-16.5g% 140-165 g/L female: 12-15g/100mL

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14
Q

Used in counting RBC, WBC, Platelet, absolute eosinophil count, sperm countWith thick cover slipImproved Neubauer Counting Chamber

A

hemacytometer

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15
Q

specific hemacytometer in the lab

A

Improved Neubauer Counting Chamber

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16
Q

of WBC in 1 cu mm of bloodDil fluid must lyze RBC

A

WBC count

17
Q

materials for WBC count

A

WBC pipetteDil fluidhemacytometer

18
Q

procedure for wbc count

A

Aspirate blood 0.5 mark. Wipe sidesAspirate RBC dil fluid up to 101 mark. Mix dil’n 1:200Discard 5-6 gttCharge counting chamberStand 5minCount RBC in HPO

19
Q

materials for differential count

A

Blood2 slidesDifferential stainPasteur pipetteCedar wood oil

20
Q

High refractive index, thereby increasing the numerical aperture of the objective lensHave specific optical and viscosity characteristics

A

cedar wood oil

21
Q

describe cover glass used in differential count

A

thicker, less flexible, surface tension of the fluid will not deform them, height of the fluid is standardized

22
Q

procedure for differential count

A

Prepare smearDry smear on flat surfaceStain smearCount 100 cells in OIO

23
Q

technique used in differential count

A

strip differential technique

24
Q

how many WBC must be counted in a smear

A

100

25
Q

devices used for DIFFERENTIAL COUNT

A

hand tally counterdifferential counter

26
Q

how many quadrants are there for WBC

A

4

27
Q

What are counted in WBC or RBC

A

dots

28
Q

how many secondary squares are in improved neubauer

A

9

29
Q

how many tertiary squares are in 1 WBC square

A

16

30
Q

what is the total number of tertiary squares of a WBC

A

16 x 4 = 64

31
Q

1 RBC square = ___ tertiary squares

A

5

32
Q

1 RBC tertiary square =___ small squares

A

16

33
Q

total number of small RBCsquares

A

16 x 5 quadrants = 80 small squares

34
Q

what are the staining solutions in differential count (list in order)

A

1st: ethanol2nd: eosin3rd: methylene blue4th: buffer or distilled water

35
Q

staining solution for fixing the slide

A

ethanol

36
Q

staining solution for staining

A

eosinmethylene blue

37
Q

staining solution for rinisng solution or stains

A

buffer or distilled water

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