HEMA LAB - HCT Determination Flashcards
tube used for adam’s microhematocrit method
capillary tube
tube used for wintrobe
wintrobe tube
tube used for haden’s modification
calibrated tube
tube used for van allen method
tube with bulb
tube used for sanford-magath method
calibrated tube, with funnel-like mouth
tube used for bray’s method
bray’s tube
anticoagulant for adam’s microhematocrit method
heparin in heparinized tube
anticoagulant for wintrobe
double oxalate
anticoagulant for haden’s modification
1.1% sodium oxalatein distilled water
anticoagulant for van allen method
1.6% sodium oxalate in distilled water
anticoagulant for sanford-magath
1.3% sodium oxalate in distilled water
anticoagulant for bray’s
heparin
normal values for bray’s method
M 47vol%
F 42vol%
normal values for sanford-magath
M 2.3 mL = 46-48%
F 2.0 mL = 40-42%
Automated methods for Hct determination
Coulter method
Autoanalyzer
normal values for Adam’s Microhematocrit method
M 40 - 54% or 0.40 - 0.54
F 35 - 49% or 0.35 - 0.49%
RPM and time for Adam’s microhematocrit method
10,000 rpm for 4-5 min
RPM and time for Wintrobe method
3000 for 30 min
RPM and time for Haden’s modification
3000 rpm for 20 min
RPM and time for Van Allen
2500 rpm, 15-30 min
RPM and time for Sanford-Magath
high speed, 15 min
RPM and time for Bray’s
none, let stand for 1 hr
what happens when there is no mixing of blood in tube prior to hematocrit method
low Hct
height of sealing clay in adam’s microhematocrit method
3 mm
1% hct = ? gm% hgb
0.34gm% hgb
1% hct = ? RBC/mm^3
107, 000 RBC/mm^3