BACTE LEC - Functions of Biochemical Media

Question 1

determination of the fermentation reactions of fastidious organisms such as Neisseria, pneumococci, streptococci, and nonsporeforming anaerobes
 used for the 
detection of bacterial motility 
and serve as a culture media for 
microorganism maintenance

Answer 1

Cystine Trypticase Agar

Question 2

enteric organisms especially Salmonella arizonae based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulphide (H2S)

Answer 2

Lysine Iron Agar

Question 3

Maintenance of Lactobacilli and for determining the action of bacteria on milk

Answer 3

Litmus Milk Medium

Question 4

Biochemical differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase

Answer 4

Moeller’s Broth

Question 5

Determination of oxidative and fermentative metabolism of carbohydrates by gram-negative bacteria
Identification of gram-negative based on ability to oxidize and ferment specific carbohydrates

Answer 5

Oxidation-Fermentation Medium

Question 6

Detection of indole production by coliforms/enterobacteriacea

Answer 6

Tryptone Broth

Question 7

Differentiates bacteria based on their fermentation of lactose, glucose and sucrose and on the production of hydrogen sulfide.
most frequently used in the identification of the Enterobacteriaceae, although it is useful for other gram-negative bacteria.

Answer 7

Triple Sugar Iron Agar

Question 8

Separating members of the family Enterobacteriaceae and some Streptococcus

Answer 8

Methyl Red Voges Proskauer Medium

Question 9

Detection of urease production, particularly by Proteus vulgaris, Micrococci and
paracolon organisms

Answer 9

Christensen Urea Agar/Stuart Urea Broth

Question 10

Differentiate members of the genera Proteus, Morganella (which were originally classified under the genus Proteus), and Providencia from other Enterobacteriaceae.

Answer 10

Phenylalanine Deaminase Agar

Question 11

Differentiation the members of Enterobacteriaceae on the basis of citrate

Answer 11

Simmon Citrate Agar

Question 12

Isolation and identification of Streptococcus pyogenes

Answer 12

PYR Agar

Question 13

Differentiation of the Shigella species from Escherichia coli and non-fermentative gram-negative bacilli

Answer 13

Acetate Slant

Question 14

Differentiation of non-fermentative gram-negative bacteria, particularly Pseudomonas
aeruginosa.

Answer 14

Acetamide Slant

Question 15

For detecting phenylalanine

deaminase,

Answer 15

phenylalanine deaminase agar