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Botany > BACTE LEC - Entero Part 3 > Flashcards

BACTE LEC - Entero Part 3 Flashcards

1
Q

principle of phenylalanine test

A

Deamination of phenylalanine to phenylpyruvic acid

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2
Q

positive rxn of the phenylalanine test

A

Positive (green color in the syneresis fluid

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3
Q

ONPG meaning

A

O-nitrophenyl-beta-D- galactopyranoside

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4
Q

test for Slow or late lactose fermenter

A

O-nitrophenyl-beta-D-galactopyranoside

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5
Q

only one that is ONPG positive

Salmonella serotype

A

Salmonella arizonae

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6
Q

rxn of citrobacter in ONPG

A

+

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7
Q

E. coli is a normal flora of the

A

GI tract

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8
Q

Closely related to Shigella (DNA homology)

A

Escherichia coli

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9
Q

“Montezuma’s revenge” “turista” Traveller’s diarrhea

A

Enterotoxigenic E. coli (ETEC)

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10
Q

diseases caused by ETEC

A

“Montezuma’s revenge”
“turista”
Traveller’s diarrhea

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11
Q

E. coli strain

Severe epidemic diarrhea = cholera Fever, headache, vomiting, chills

A

Enterotoxigenic E. coli (ETEC)

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12
Q

E. coli strain

Disease = Shigellosis

A

Enteroinvasive E. coli (EIEC)

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13
Q

Invasion of intestinal epithelium

E. coli strain

A

Enteroinvasive E. coli (EIEC)

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14
Q

E. coli strain

Fever, cramps, bloody stool w/ RBCs, neutrophil and mucus

A

Enteroinvasive E. coli (EIEC)

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15
Q

Verotoxin causes what diarrhea

A

bloody diarrhea

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16
Q

Sorbitol negative SMAC

A

Enterohemorrhagic E. coli (EHEC)

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17
Q

Verotoxic E. coli (VTEC)
0157 H7



A

Enterohemorrhagic E. coli (EHEC)

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18
Q

E. coli strain

Hemolytic uremic syndrome

A

Enterohemorrhagic E. coli (EHEC)

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19
Q

E. coli strain

Hemorrhagic colitis

A

Enterohemorrhagic E. coli (EHEC)

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20
Q

strain number of Enterohemorrhagic E. coli (EHEC)

A

0157 H7

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21
Q

Resembles E. coli
Lactose negative & nonmotile
Shigellosis (bacillary dysentery)

A

Shigella

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22
Q

Shigella: lactose (-) or (+)?

A

Lactose negative

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23
Q

Motility of Shigella

A

nonmotile

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24
Q

disease caused by shigella

A

shigellosis

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25
Q

bacillary dysentery

A

shigellosis

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26
Q

with fishy odor

A

shigella

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27
Q

enteric bacteria which causes Bloody, mucoid, diarrheic stool w/ pus cells & fishy odor

A

shigella

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28
Q

significant shigella species [4]

A

S. sonnei
S. dysenteriae
S. flexneri
S. boydii

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29
Q

Shigella spp
Most prevalent in the US
Neurotoxin & enterotoxin
Non-mannitol fermenter

A

S. dysenteriae

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30
Q 
Shigella spp. 
Beta galactosidase (ONPG +) ODC +
A

S. sonnei

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31
Q

Motile & ODC Positive
Large amount of gas from glucose


A

Enterobacter

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32
Q

Enterobacter spp

Predominant isolate UTI & wound infection

A

E. cloacae

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33
Q

Enterobacter spp
Resembles E. cloacae
Yellow pigmented Neonatal sepsis

A

E. sakazakii

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34
Q 
Short & nonmotile 
encapsulated
Large, mucoid colonies 
Strings out when touched
by the needle
A

Klebsiella

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35
Q

Klebsiella

Bacterial pneumonias

A

K. Pneumoniae

36
Q

Kleb spp.

Blood & stool culture Indole positive

A

K. oxytoca

37
Q

Kleb spp.
Atrophy of the nasal mucous membranes w/ fetid odor
Blood & stool

38
Q

Kleb spp.
Rhinoscleroma Destructive granuloma of the
nose & pharynx


A

K. rhinoscleromatis

39
Q

impt spp of klebsiella [poor]

A

pneumonia
oxytoca
ozanae
rhinoscleromatis

40
Q

impt spp of serratia [morl]

A

marcescens
liquefaciens
odorifera
rubidaea

41
Q

Opportunistic pathogens
Deoxyribonuclease, lipase, gelatinase
red pigment

42
Q

serratia spp.

Pneumonia & septicemia

A

S. marcescens

43
Q

serratia spp.

Rancid, potato-like odor

A

S. odorifera

44
Q

serratia spp.

Ferment arabinose

A

S. liquefaciens

45
Q

Negative to Lactose, sucrose, sorbitol Citrate, DNAse, lipase

A

Hafnia alvei

46
Q

Contaminated IV fluids
Yellow pigment
Negative to ADH, LDH, ODC

A

Pantoea agglomerans

47
Q

number of serotypes of salmonella

48
Q

very complex genus

A

salmonella

49
Q

classification of salmonella serotypes

A

Kauffman - White

50
Q

enteric [typhoid] fever lasts for __

51
Q

appearance of salmonella on BSA and BGA

A

Metallic colonies with black ring on bsa

52
Q

sx for disease by salmonella - 1st and 2nd week

53
Q

sx for disease by salmonella - 3rd and 4th week

54
Q

sx for disease by salmonella - indefinite

55
Q

clinical diseases induced by salmonella

A

septicemia
enteric fever
gastroenteritis

56
Q

antigen test for salmonella

A

widal test

57
Q

o antigen in widal test meaning

A

active infection

58
Q

h antigen in widal test meaning

A

past immunization or infection

59
Q

Salmonella spp w/c causes
Enteric fever
Typhoid & paratyphoid

60
Q

Salmonella spp w/c causes

septicemia

A

S. choleraesius

61
Q

Salmonella spp w/c causes

gastroenteritis

A

S. typhimurium

S. enteritidis

62
Q

salmonella test
Amebocytes of horse crab (visible gel)
endotoxin

A

linulus test

63
Q

Ballooning of the intestinal tract or ileal loop (accumulation of fluid)
enterotoxin

A

ileal loop test

64
Q

2 genera that citrobacter resembles

A

salmonella

e. coli

65
Q

2 impt spp of citrobacter

A

freundii

diversus

66
Q

tribe of Proteus Providencia Morganella

67
Q

tribe proteeae
Rapid urease activity “swarming” motility
on BA


68
Q

Source of GI infection W
ound infections abscesses
Trauma or accidents (aquatic environment)
Biochemically similar to E. coli

A

Edwardsiella tarda

69
Q

Abundant H2S in TSI agar
Lactose negative
LDC & ODC positive ADH negative

A

Edwardsiella tarda

70
Q

size/appearance of yersinia on MAC

71
Q

morphology of yersinia

A

coccobacilli

72
Q

yersinia spp motile at RT

73
Q

genus: nonmotile at 37C

74
Q

yersinia spp which
causes enterocolitis
Ingestion of contaminated water, pork, beef or milk products
Fecal-oral transmission

A

Y. enterocolitis

75
Q

Ferments mannitol and appears as red, “bull’s eye” colonies w/ colorless halo
CIN medium

A

yersinia enterolitica

76
Q

types of plague by y. pestis

A

bubonic, septicemic, pneumonic

77
Q

causes plague [black death]

78
Q

vector of y. pestis

A

flea [through a bite]

79
Q

yersinia with safety pin appearance

A

yersinia pestis

80
Q

yersinia

“Stalactite patterns”

A

yersinia pestis

81
Q

yersinia spp.

ferments both sucrose & sorbitol

A

yersinia enterolitica

82
Q

yersinia spp.

fails to ferment both sucrose & sorbitol

A

yersinia pseudotuberculosis

83
Q

Yersinia spp.
Wild animals & game fowl
Lymphadenitis in children
ODC negative Fails to ferment both sucrose & sorbito

A

Y. pseudotuberculosis

83
Q

Citrobacter species
H2S +
KCN +

84
Q

Citrobacter species

Indol +

A

C diversus

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