Biochem Lab Color Rxns Flashcards
purpose of biuret test
detect presence of peptide bonds
purpose of ninhydrin
presence of alpha AA
purpose of xanthoproteic
presence of aromatic AA
purpose of millon’s
presence of tyrosine (phenolic group)
purpose of hopkins-cole
presence of indole group of tryptophan
purpose of sakaguchi
un or monosubstituted guanidine in arginine
purpose of nitroprusside
presence of cysteine
purpose of fohl’s
presence of sulfur-containing aa
purpose of test for amides
R groups of glutamine and arginine
purpose of pauly
test for tyrosine and histidine
(+) result of biuret
violet solution
(+) result of ninhydrin
blue-violet sol’n
(+) result of xanthoproteic
yellow ppt/soln
(+) result of millon’s
red/reddish-brown solution
(+) result of hopkins-cole
purple ring at junction where 2 liquids meet
(+) result of sakaguchi
red coloration
(+) result of nitroprusside
red soln
(+) result of fohl’s
black or brown sediment
(+) result of test for amides
red litmus to blue
evolution of gas
(+) result of pauly test
red coloration
reagents used in biuret
- 5 M NaOH
0. 1 M CuSO4
reagents used in ninhydrin
0.1% ninhydrin solution
reagents used in xanthoproteic
Conc. HNO3
Conc. NaOH
reagents used in millon’s
Millon’s reagent
reagents used in hopkins-cole
Hopkins Cole reagent
H2SO4
reagents used in sakaguchi
10% NaOH
0.02% Naphthol solution
2% NaOBr
reagents used in nitroprusside
3M NaOH
2% Nitroprusside
reagents used in fohl’s
30% NaOH
5% (CH3COO)2Pb
reagents used in test for amides
20% NaOH
reagents used in pauly
Diazo reagent (1% sulfanilic acid + 5% NaNO2 solution) 10% Na2CO3
