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Botany > Biochem Lab Color Rxns > Flashcards

Biochem Lab Color Rxns Flashcards

1
Q

purpose of biuret test

A

detect presence of peptide bonds

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2
Q

purpose of ninhydrin

A

presence of alpha AA

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3
Q

purpose of xanthoproteic

A

presence of aromatic AA

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4
Q

purpose of millon’s

A

presence of tyrosine (phenolic group)

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5
Q

purpose of hopkins-cole

A

presence of indole group of tryptophan

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6
Q

purpose of sakaguchi

A

un or monosubstituted guanidine in arginine

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7
Q

purpose of nitroprusside

A

presence of cysteine

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8
Q

purpose of fohl’s

A

presence of sulfur-containing aa

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9
Q

purpose of test for amides

A

R groups of glutamine and arginine

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10
Q

purpose of pauly

A

test for tyrosine and histidine

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11
Q

(+) result of biuret

A

violet solution

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12
Q

(+) result of ninhydrin

A

blue-violet sol’n

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13
Q

(+) result of xanthoproteic

A

yellow ppt/soln

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14
Q

(+) result of millon’s

A

red/reddish-brown solution

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15
Q

(+) result of hopkins-cole

A

purple ring at junction where 2 liquids meet

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16
Q

(+) result of sakaguchi

A

red coloration

17
Q

(+) result of nitroprusside

A

red soln

18
Q

(+) result of fohl’s

A

black or brown sediment

19
Q

(+) result of test for amides

A

red litmus to blue

evolution of gas

20
Q

(+) result of pauly test

A

red coloration

21
Q

reagents used in biuret

A
  1. 5 M NaOH

0. 1 M CuSO4

22
Q

reagents used in ninhydrin

A

0.1% ninhydrin solution

23
Q

reagents used in xanthoproteic

A

Conc. HNO3

Conc. NaOH

24
Q

reagents used in millon’s

A

Millon’s reagent

25
Q

reagents used in hopkins-cole

A

Hopkins Cole reagent

H2SO4

26
Q

reagents used in sakaguchi

A

10% NaOH
0.02% Naphthol solution
2% NaOBr

27
Q

reagents used in nitroprusside

A

3M NaOH

2% Nitroprusside

28
Q

reagents used in fohl’s

A

30% NaOH

5% (CH3COO)2Pb

29
Q

reagents used in test for amides

A

20% NaOH

30
Q

reagents used in pauly

A 
Diazo reagent (1% sulfanilic acid + 5% NaNO2 solution)
10% Na2CO3

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