Bacte Lec - Stains Flashcards
Capsule color
Hiss stain
light violet
Capsule color
Tyler stain
light violet
Capsule color
Muir stain or Welchs
light violet
capsule and background color
India ink or nigrosin
capsule: Unstained
bg: black
capsule and background color
Congo red
capsule: Unstained
bg: blue
capsule and background color
Anthony’s stain
capsule: Unstained
bg: purple
granule color
Albert stain
Blue black
Neisser stain granule color
Dark blue
Lindegren’s stain granule color
Reddish brown
Loeffler’s alkaline methylene blue (LAMB) granule color
dark blue
Wayson’s stain
bipolar bodies (appear to be spore) are red
Stain for flagella (4)
- Leifson stain
- Gray’s method
- Silver technique
- Caesares Gil
endospore color
Fulton Schaeffer’s method
green
endospore color
Dorner’s stain
red
endospore color
Wirtz Conklin
green
Stain for Rickettsia (3)
- Castaneda – blue
- Machiavelo – red
- Giemsa – blue
Stain for Chlamydia (3)
- Gimenez – inclusion bodies are red
- Machiavello
- Giemsa
Stain for spirochetes
Fontana Tribondeau
dark brown or black spirochetes
Levaditi Silver Impregnation
– black
India Ink negative stain
– unstained spirochetes, black background
Stain for mycoplasma
• Dienes stain –
blue
Stain for cell wall
• Victoria blue dye –
blue
Stain for DNA
• Feulgen stain –
greenish or with green tinge
chemical used in Kinyoun’s method
tergitol
function of tergitol
reduces surface tension, promotes penetration or attachment
alters decolorizer by weakening it to differentiate these 2 species: M. tuberculosis and M. smegma
Pappenheim’s method
method that differentiates M. tuberculosis and M. leprae
Baumgarten’s method
stains M. leprae in tissue sections
Wadefite Technique
staining technique for bacteria in tissue sections
Brown and Brenn technique
modified Grams stain
Brown and Brenn technique
stains for flagella (4)
Leifson Stain
Caesares Gil
Silver technique
Gray’s method
Stains for acid-fast bacilli
Ziehl-Neelsen Kinyoun's Truant's fluorescence technique Flickerson and Kammer's fluorescence technique Pappenheim method Baumgarten's method Wadefite technique
acid-fast bacilli stain that differentiates between M. leprae and M. tuberculosis
Baumgarten’s method
Baumgarten’s method differentiates these two species
M. leprae and M. tuberculosis
Pappenheim’s method differentiates these 2 species
M. tuberculosis and M. smegma
color of M. tuberculosis in Pappenheim’s method
red
color of M. smegma in Pappenheim’s method
blue
color of M. leprae in Baumgarten’s method
red
color of M. tuberculosis in Baumgarten’s method
blue
