Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Botany > Bacte Lec - Culture Media Lecture > Flashcards

Bacte Lec - Culture Media Lecture Flashcards

1
Q

bacterial nutritional requirements (3)

A

water
energy source
carbon source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

types of organisms based on their source of energy

A

phototrophs

chemotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

types of organisms based on their carbon source

A

heterotroph

autotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

types of chemotroph

A

lithotrophs

organotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

types of organisms based on their carbon source

require CO2

A

autotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

types of organisms based on their carbon source

require organic compounds

A

heterotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

types of organisms based on their energy source

require light

A

phototrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

types of organisms based on their energy source

require chemicals

A

chemotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

prevention of bacterial growth by water removal

A

lyophilization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

organisms which need co2

A

capnophiles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

examples of capnophiles

A

Haemophilus influenzae

Neisseria gonorrhoea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

energy source: light

C source: CO2

A

photoautotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

energy source: light

C source: organic compounds

A

photoheterotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

energy source: inorganic compounds

C source: CO2

A

chemoautotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

energy source: organic compounds

C source: organic compounds

A

chemoheterotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

photoautotrophs

energy source

A

light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

photoheterotrophs

energy source

A

light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

chemoautotrophs

energy source

A

inorganic compounds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

chemoheterotrophs

energy source

A

organic compounds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

chemoheterotrophs

c source

A

organic compounds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

chemoautotrophs

c source

A

co2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

photoheterotrophs

c source

A

organic compounds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

photoautotrophs

c source

A

CO2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Representative bacteria of photoautotrophs

A

cyanobacteria
photosynthetic bacteria
algae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Representative bacteria of photoheterotrophs

A

photosynthetic bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Representative bacteria of chemoheterotrophs

A

protozoa, fungi, bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Representative bacteria of chemoautotrophs

A

sulfur, iron, ammonia oxidizing bacteria

methane producing bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

essential elements needed by microorganisms [TOM]

A

Metals
Trace Elements
Organic Growth Factors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

metals needed by microorganisms

A

K, Mg, Ca, Fe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Trace elements needed by microorganisms

A

Molybdenum
Copper
Cobalt
Zinc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Organic growth factors

A

purine and pyrimidine
amino acids
vitamins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

elements of culture media [6]

A 
free of inhibitory substances
proper ph
sufficient moisture
desired consistency
initially sterile
right nutrients
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

classification of CM according to composition

A

synthetic, chemically defined

non-synthetic, chemically undefined, complex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

classification of CM according to composition

specific substances can be identified

A

synthetic, chemically defined

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

examples of synthetic, chemically defined

A

nutrient agar/broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

classification of CM according to composition

specific substances cannot be identified

A

non-synthetic, chemically undefined, complex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

ex of non-synthetic, chemically undefined, complex

A

yeast extract, beef extract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

digested proteins rich in protens, amino acids, nitrogen and carbon source

A

peptone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

form of dehydrated culture media

A

granular

powder

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

glassware for preparation of plated cm

A

erlenmeyer flask

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

glassware for preparation of tubed cm

A

beaker

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

classification of CM according to physical state

A

solid
liquid
semi-solid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

agar composition in solid cm

A

1.5 -2 %

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

agar compostion in semi solid

A

0.5 - 1%

45
Q

complex polysaccharide [marine algae]

A

agar

46
Q

temp of liq agar

A

100c

47
Q

temp of solid agar

A

40C

48
Q

adv of albumin as solidifying agent

A

coagulates at high temperature

49
Q

solidifying agent: coagulates at high temperature

A

albumin

50
Q

solidifying agent: diagnostic medium

A

gelatin

51
Q

3 solidifying agent

A

agar
albumin
gelatin

52
Q

ex of semisolid medium

A

SIM

53
Q

semisolid medium which determines motility or ability to produce hydrogen sulfide and also for indole detection

A

Sulfide Indole Motility

54
Q

agar composition of liquid medium

A

0%

55
Q

merely a solidifying agent

A

agar agar

56
Q

if you are to prepare 100 ml of a certain culture media, how much in g of agar agar should you add

A

1.5 - 2g

57
Q

classif of cm according to function

A 
>general isolation/supportive/simple/basal/ordinary
>enriched/nonselective
>selective
>differential
>enrichment
58
Q

classif of cm according to function

non-fastidious bacteria

A

general isolation/supportive/simple/basal/ordinary

59
Q

classif of cm according to function

fastidious bacteria

A

enriched/nonselective

60
Q

nutrient supplements placed in an enriched or nonselective culture medium

A

blood
ascitic fluid
serum

61
Q

difference between enriched and enrichment

A

enriched - promotes growth of all organisms

enrichment - promotes growth of organism of interest

62
Q

difference between CAP and BAP

A

BAP - blood is added when the basal medium has cooled

CAP - blood is added when the basal medium is hot, which causes lysis of cells, attributed to the chocolate color

63
Q

temp when to added Blood in nutrient agar

A

40-45C

64
Q

basal medium of bap and cap

A

nutrient agar

65
Q

types of blood [3]

A

5% defibrinated sheep’s blood
horse blood
outdated human blood

66
Q

types of blood

for fastidious like Haemophilus

A

5% defibrinated sheep’s blood

67
Q

types of blood

for differentiation of various types of hemolysis

A

5% defibrinated sheep’s blood

68
Q

Media with solid and liquid

A

biphasic

69
Q

type of hemolysis

no hemolysis

A

gamma hemolysis

70
Q

type of hemolysis

complete hemolysis

A

beta hemolysis

71
Q

type of hemolysis

partial hemolysis

A

alpha hemolysis

72
Q

type of hemolysis

greenish discoloration that surrounds a bacterial colony

A

alpha hemolysis

73
Q

___ hemolysis is characteristic of Streptococcus pyogenes and some strains ofStaphylococcus aureus.

A

beta

74
Q

___ hemolysis is characteristic of Streptococcus pneumonia

A

alpha

75
Q

type of blood

gives erratic or consistent results

A

horse blood

76
Q

disadv of outdated human blood

A

contains: antibacterial substances, excess citrate ions, other substances (antibodies)

77
Q

agar which contains heated blood or enzyme treated blood

A

chocolate agar

78
Q

CAP contains what type of blood

A

heated blood or enzyme treated blood

79
Q

anticoagualant for blood

A

citrate phosphate dextrose [CPD] or CPD A

80
Q

prevents clotting

A

defibrination

81
Q

what to place in an EF to prevent clotting/defibrination

A

paper clips or marbles [fibrin will adhere to these]

82
Q

classif of cm according to function

distinguishes between organisms based on appearance on the medium

A

differential medium

83
Q

classif of cm according to function

distinguishes between lactose fermenters and non-lactose fermenters

A

differential medium

84
Q

classif of cm according to function

contains indicators

A

differential medium

85
Q

classif of cm according to function

enhances bacterial growth of desired bacteria

A

selective medium

86
Q

inhibitory substances in selective medium

A 
bile salts
sodium azide
sodium desoxycholate
potassium tellurite
gentian violet
antibiotics
87
Q

blood agar can be enriched and differential

A

enriched: addition of blood
differential: differentiates hemolytic reactions

88
Q

classif of cm according to function

tinsdale agar

A

selective

89
Q

classif of cm according to function

MSA

A

selective

90
Q

classif of cm according to function

SSA

A

selective

91
Q

classif of cm according to function

LJ

A

selective

92
Q

Components of transport medium

A

buffer and salts

93
Q

special cm

maintain viability w/o altering concentration

A

transport medium

94
Q

special cm

temporary storage of chemical sx

A

transport medium

95
Q

special culture medium

enhances growth of bacteria that may be overgrown by competitors

A

enrichment broth

96
Q

special cm

layers of living cells

A

cell line/tissue culture

97
Q

special cm

series of battery test medium

A

biochemical test media

98
Q

examples of biochemical test media [3]

A

enterotube
API Test OR API Medium
Packaged Test System

99
Q

series of color reactions matched with a color chart with an interpretation

A

biochemical test media

100
Q

added when an organism is suspected to be an anaerobe

A

mineral oil

101
Q

color of nonfermenter

A

red

102
Q

color of fermenter

A

yellow

103
Q

displacement of agar interpretation

A

gas production capability of organism

104
Q

blackening of butt interpretation

A

H2S production

105
Q

small tubes used to detect gas production

A

Durham tube

106
Q

growth on the bottom of thioglycollate broth

A

anaerobe

107
Q

growth on the surface of thioglycollate broth

A

aerobe

108
Q

growth on the middleof thioglycollate broth

A

microaerophilic

109
Q

indicates that organism is toxic

A

cytopathic effect (CPE) or cell death

Botany (324 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions