CC Lec - Specimen Flashcards
3 sources of blood
capillary puncture
venipuncture
arterial puncture
best method of blood collection for newborns and babies
capillary or skin punc
method of blood collection seldom used for aged individuals
capillary or skin punc
method of blood collection wherein sx usually used for chemistry analysis
venipuncture
sites of puncture in venipuncture
basilic vein
cephalic vein
median vein
vein for venipuncture pointed toward the body
basilic vein
vein for venipuncture pointed away the body
cephalic vein
used in the analysis if pO2, pCO2, blood pH
arterial blood
method of blood collection not done by ordinary RMTs
arterial puncture
sites of arterial puncture
brachial, femoral, radial artery
how to label specimen
last name, first name, MI
specimen or patient no.
when to label specimen
after collection/filling up the tube
number 1 reason of specimen rejection
hemolysis
in serum what ions are abundant
Na and Cl
inside cell what ions are abundant (and their ratios)
K (20:1)
Mg (10:1)
hemolysis of RBC leads to
false elevation of analyte concentration
straw colored yellow fluid
serum
liquid portion of clotted blood
serum
temperature of blood for test of CK
cold temp
temperature of Sx for LD
room temp
reasons of rejection
questionable labelling
hemolysis
QNS
Substance added to blood to prevent clotting
Anticoagulant
mechanism of action of anticoagulant
1) can precipitate, bind, complex Ca thus Ca is removed
2) by acting as antithrombin
stages of clotting process
generation of thromboplastin
conversion of prothrombin to thrombin
conversion of fibrinogen to fibrin clot
Ca dependent stage of clotting process
generation of thromboplastin
Thromboplastin dependent stage of clotting process
conversion of prothrombin to thrombin
thrombin dependent stage of clotting process
conversion of fibrinogen to fibrin clot
gray stoppered tube consists of what chemicals
NaF
Iodoacetate
antiglycolytic tube
gray
antiglycolytic agent inhibits enolase
NaF
antiglycolytic agent inhibits glyceraldehyde-3-phosphate-dehydrogenase
Iodoacetate
substance in green top
heparin
natural anticoagulant best for preventing clotting of arterial blood
heparin
brand names of edta
versene, sequestrene
anticoagulant important for HbA1c measurement
EDTA
Most common tube used in Chemistry
red top
yellow tube contains
SPS (sodium polyanethol sulfonate
tube/top used for blood culture
yellow top
top w/c contains thrombin, used in hematology
orange
top w/o anticoagulant but with separator gel
marbled top/red/gray top
contains citrate
blue top
top/tube best for blood units used in transfusion
blue top
order of draw in chemistry
YRB Green Lavender Gray
order of draw in coagulation
YBR Green Lavender Gray
gauge number of needle in arterial puncture
gauge 18
color rgt for glucose
orthotoluidine
color rgt for urea
diacetylmonoxide
color rgt for protein
alkaline copper tartrate
color rgt for creatinine
alkaline picrate
color rgt for cholesterol
acetic anhydride sulfuric acid
