GPHT LAB - Adv and Disadv Part 2

Question 1

adv

prevents formation of troublesome post mortem precipitated acid formalin pigment

Answer 1

10% buffered formalin

Question 2

disadv

preserves poorly the RBC

Answer 2

Heidenhain’s Susa solution

Question 3

disadv

prolonged fixation of large specimens

Answer 3

Heidenhain’s Susa solution

Question 4

adv

excellent as routine fixative

Answer 4

formol sublimate

formol saline sublimate

Question 5

adv

produces little or no shrinkage of tissues

Answer 5

formol sublimate

formol saline sublimate

Question 6

disadv

does not preserve the thick slices of tissues thus not to be used for more than 1 cm thick specimen sections

Answer 6

formol sublimate

formol saline sublimate

Question 7

adv and disadvantages are similar to formol sublimate

Answer 7

formol saline sublimate

Question 8

adv

permits excellent staining of nuclei

Answer 8

zenker’s solution

Question 9

adv

recommended for tissues to be stained by one of the trichrome technique

Answer 9

zenker’s solution

Question 10

disadv

not stable after the addition of acetic acid

Answer 10

zenker’s solution

Question 11

adv

gives excellent nuclear fixation

Answer 11

zenker’s formol

Question 12

adv

preserves cytoplasmic granules well

Answer 12

zenker’s formol

Question 13

adv

excellent fixative for pituitary and BM tissue

Answer 13

zenker’s formol

Question 14

disadv

prolonged fixation of tissue produces brown scum

Answer 14

zenker’s formol

Question 15

adv

yellow stain is useful when handling fragments of tissues

Answer 15

bouin’s solution

Question 16

disadv

should never be used for preserving kidneys because of extreme distortion

Answer 16

bouin’s solution

bouin’s fluid

Question 17

adv

excellent for nuclear elements (chromosomes)

Answer 17

flemming’s fluid

Question 18

adv

permanently preserves fats

Answer 18

flemming’s fluid

Question 19

disadv

deteriorates rapidly thereby it must be prepared immediately before its use

Answer 19

flemming’s fluid

Question 20

adv

fixes and dehydrates simultaneously

Answer 20

carnoy’s

Question 21

adv

most rapid of all fixatives

Answer 21

carnoy’s

Question 22

disadv

causes excessive shrinkage and hemolysis of RBC

Answer 22

carnoy’s

Question 23

disadv

only small pieces of tissues may be used

Answer 23

carnoy’s

newcomer’s

Question 24

adv

gives better nuclear fixation than flemming’s fluid

Answer 24

bouin’s fluid

Question 25

adv

useful for minute fragments as the yellow color given to tissue makes it easier to locate the specimen

Answer 25

bouin’s fluid

Question 26

disadv

cytoplasmic structres such as mitochondria are distorted or dissolved

Answer 26

bouin’s fluid

Question 27

adv

tissue require only one change of absolute alcohol before clearing

Answer 27

newcomer’s fluid

Question 28

adv
only small amount of fixative is required
5-10x the bulk of the tissue

Answer 28

champy’s fluid

Question 29

disadv similar to flemming’s fuid

Answer 29

champy’s fluid

Question 30

adv

more penetrating than chromium fixatives

Answer 30

regaud’s fluid (moller)

Question 31

disadv

preserves fats

Answer 31

regaud’s fluid (moller)

Question 32

disadv

needs post-chroming

Answer 32

regaud’s fluid (moller)

Question 33

adv

demonstrates Rickettsia and other bacteria

Answer 33

orth’s fluid

Question 34

adv

preserves myelin better than buffered formalin

Answer 34

orth’s fluid

Question 35

disadv similar to regaud’s fluid

Answer 35

orth’s fluid