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Botany > CC Lab - Spectrophotometry > Flashcards

CC Lab - Spectrophotometry Flashcards

1
Q

major categories of analytical techniques

A

spectrometry
luminescence
electroanalytical method
chromatography

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2
Q

main principle of spectro

A

beer’s law/beer-lambert’s law

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3
Q

3 major methods for calculation of unknown solution

A

direct comparison/ratio of standard to unknown
use of standard curve
use of molar absorptivity value

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4
Q

source of reagent for exp

A

reagent kit

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5
Q

3 substances found in reagent

A

catalyst
enzyme
buffer

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6
Q

type of spectrophotometer in the lab

A

Humalyzer Primus

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7
Q

old spectrophotometer

A

ALS 2000

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8
Q

Conventional parts of spectrophotometer (6)

A 
exciter lamp
variable slit diaphragm
monochromator
sample holder
photodetector
read out device
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9
Q

parts of spectrophotometer

light source

A

exciter lamp

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10
Q

parts of spectrophotometer

separates radiant energy

A

monochromator

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11
Q

parts of spectrophotometer

isolate narrow band of light

A

variable slit diaphragm

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12
Q

wavelength of glucose

A

500 nm

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13
Q

units of wavelength

A

nm or mu or 10^-9m

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14
Q

parts of spectrophotometer

holds smaple

A

sample holder/cuvetter

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15
Q

shape of cuvette

A

round or square

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16
Q

parts of spectrophotometer

reads amount of transmitted light then converts it to an electrical signal

A

photodetector

17
Q

parts of spectrophotometer
galvanometer
converts electric signal to a readable data

A

read out device

18
Q

keypad (4 commands)

A

ESC
Enter
Rinse
Paper

19
Q

rinsing solution

A

triple distilled water

20
Q

wait how many min after turning on the spectrophotometer

A

5 min

21
Q

2 types of operating procedure

A

Kinetic or rate method

Endpoint method

21
Q

2 types of operating procedure

A

Kinetic or rate method

22
Q

Operating procedure for ALS 2000 Spectrophotometer

A

Endpoint method

23
Q

No sx

To set reading at 0

A

Blank

24
Q

Blank solution with reagent

A

Reagent blank

25
Q

Blank soln with only the sample, no rgt

A

Serum or sample blank

26
Q

Empty test tube to set reading at zero

A

Air blank

27
Q

Tool for precision which BEHAVES LIKE A SPECIMEN

A

Controls

Botany (324 decks)

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