Eating habits lab - 2nd set

Question 1

acid fast stain

Answer 1

differential stain

Question 2

mycobacterium and norcardia sp.

Answer 2

acid-fast. due to waxy mycolic acid in their cell wall. gram’s stain indeterminate when we do grams stain despite having gram positive cell wall structure.

Question 3

bacteria used in acid fast ex.

Answer 3

tuberculosis and leprosy (hansen’s disease)

Question 4

acid fast bacteria positive

Answer 4

red

Question 5

non-acid fast bacteria

Answer 5

a fast negative, blue.

Question 6

we use

Answer 6

s megamitis, non-pathogenic.

Question 7

what is the primary acid fast stain?

Answer 7

carbolfuschin - basic dye, cationic

Question 8

fix cell..

Answer 8

steam for 3-5 min. dye already has phenol.

Question 9

mordant melts

Answer 9

mycolic acide cell wall (acid fast)

Question 10

after fix..

Answer 10

cool slide for a few minutes carbon dye trapped inside cells. add decolorizing, no time limit. add counterstain, methalyin blue. wash off, dry, observe.

Question 11

tb

Answer 11

rod-shaped, acid fast bacteria

Question 12

virulence of tb

Answer 12

keeps virus clumped together. defense cells in body can’t eliminate

Question 13

non acid-fast stains…

Answer 13

lose primary stain when you use alcohol

Question 14

what is mordant in acid fast?

Answer 14

steaming plus phenol

Question 15

staph, streplococcus, s. aurus

Answer 15

non spore