Eating habits - 7th set (meta) Flashcards
Electron Transport Chain (body weight)
- the most productive stage of energy harvesting - maximum amount of ATP synthesized. you make daily body weight in ATP
- involves collection of membrane associated carriers
not symmetrical (some are integral, some are peripheral proteins) arranged and pass e- from one to next member
2 classes of carriers - electron transport chain
some accept electrons only from previous member
ii. …others accept electron, proton pair (e and H) - (then the E is passed to the next member, and then the proton is pumped across the membrane. then it generates a proton gradient at this time)
the proton is pumped across the
the membrane - generates a proton gradient -
> functions unidirectionally
> some functions as proton pumps
prokaryotes, etc operates at the
plasma membrane level = surrogate mitochondria. eukaryotes - inner mitochondrial membrane.
as electrons pass down the electron transport system..
trapped energy is released and ATP is generated
electron carriers (names)
flavoproteins, metal containing proteins, ubiquinone, cytochromes
Flavoproteins - electron carrier
integral proteins, have a riboflavin derivative associated with it - called FMN (flavin mononucleotide)
Metal containing proteins - electron carrier
integral proteins - either iron or sulfur associated with it. elements (like Fe). Fe alternates between oxidized and reduced state.
Ubiquinone - electron carrier
non-protein, small organic lipid soluble molecules. sandwiched between lipid layers. in E. coli, its called menaquinone - it gets converted to vitamin K in a few steps.
Cytochromes - electron carrier
mixed proteins (integral or peripheral) with Fe and heme associated w/ their structure. Fe is the one that alternates between an oxidized and a reduced form. electrons usually passed to cytochrome B - then C - the A - then A3.
collagen is similiar
in animals and humans
fermentation (bread)
no 02, glycolysis only, organic molecule either pyruvate (made during glycolysis) - becomes lactic acid or acetaldehyde, commercially important
used by organisms that cannot respire; no oxygen usage
- ATP synthesized during glycolysis only, thus low yield - only a few atp made by substrate level phosphorylation.
- there is NO Krebs cycle or ETC reactions
- terminal electron acceptor (along with protons from NADH) is a organic molecule - not inorganic (this is associated w/ respiration)
(either pyruvate or it’s derivative - then it becomes lactic acid or acetaldehyde (if it undergoes decarboxylation)
- end products are used as markers for identification and are commercially important products
function of fermentation
to generate steady supply of NAD+, which is recycled. to convert NADH back into the coenzyme NAD+ so that it can be used again
metabolism definition
the sum of all chemical reactions inside cells.
respiratory has___pathways
has all 3 pathways, linear, branched , cylindrical
electrochemical gradient (name split)
2 parts - it is a charge and a chemical compound. the gradient has the potential energy = proton motive force (the cell is a tiny battery), it’s trapped to make atp.
chemosmotic theory (synthase)
2 important things - 1. protons (H) can’t just diffuse membrane, only through pores and channels. 2. aligned by atp synthase - can only get through there.
fenfen
leaky mitochondria. co2 poisoning stops E transport chain
cytochrome a and a3 (when together) - form
if bacteria have cytochrome oxidase, they are called oxidase positive cells. some includes niessera gonorrhea and pseudomonas.
some have a and a3 separate - called oxidase negative cells. ex. E.coli, salmonella, proteus
bacteria and animal has stayed the same
cytochromes
once the proton is passed to the next member, it gets…
reoxidized
cytochromes are similar in..
humans and animals. it is evolutionary conserved.
when FAD accepts a proton electron pair…
as a hydrogen atom, it gets reduced to FADH2
NAD accepts the electron…
it becomes NADH and drops first member flavoprotein. flavo is accepting electron proton pair. these protons are pumped across the membrane.
flavoprotein delivers electron to…
ubiquinone
ubiquione not a proton..
pump
when the carrier can pump the proton across the membrane..
it creates a chemical gradient