Eating habits - 14th set (micr. growth cont) Flashcards
Antagonistic ( harmful)
Virus “killing” of host cell following replication
Synergistic (beneficial) (mae - symb)
symbiotic and complex
Symbiotic relationship and ex. (think intestines)
organisms lives in close contact ( physically or nutritionally); individually are interdependent Ex: E.coli inhabitation of intestine
Complex relationship (aka. biofilm) (help but %)
are cooperative; benefits are amplified
In humans, biofilms plays a major role in 65% of infections
Biofilms in households
kitchen sink, toilet, shower
health issues - biofilms (body parts) (PECK) (when you were small)
prostatitis, kidney infection, otitis media (ear infection), cystic fibrosis
Cooperative interactions allow species to.. (biofilms)
grow which wouldn’t have grown in the 1st place
Biofilms develops as a…(slime - matrix made of DPG - dan on biofilm)
slimy matrix which allows cells as well as the biofilm to attach. Matrix is made of,
- DNA
- proteins
- fibers of glycocalyces - capsule
Biofilm (with associated matrix ) protects members from…(stress - denver)
environmental stress ( uv radiation), antimicrobial drugs including antibiotics, pH alterations, temperature and humidity changes
concentrate and conserve digestive enzymes (biofilm) (digest absorb)
bacteria tend to absorb nutrients. digestive enzyme helps in the breakdown and facilitates the nutrient absorption.
quarum sensing (biofilm) - and can be both…. (quarrel over car)
= molecules, autoinducers. gram + and gram -.
autoinducers (car - diffused - reporter)
secreted molecules that bind to bacterial cell (target) - diffuses across membrane - binds and activates, transcription factors (TF) . TF are involved with genes involved with biofilm production.
Quorum sensing and biofilms (quarrel w/ neighbor)
Quorum sensing is the communication between members - cells responds to neighboring cell’s density and secretes molecules
Planktonic showers (en mass and ex)
When large # of cells leaves the biofilm and disseminate ex. Cystic fibrosis patients lungs and Pseudomonas sps
Cystic fibrosis patients lungs and Pseudomonas sps (secretes outside poly)
the bacteria secretes large amount of
exopolysaccharide (capsule polysaccharides) alginate that causes, - persistent respiratory infections - aggravates airway blockage - makes breathing difficult.
rifamyacin targets…and treats what disease? (fav again)
RNA polymerase of TB - cant be used to treat e.coli due to rapid turnover
rifamycin can bind…(opposite)
and inhibit rna polymerase
ex. of complex relationships (tu’s dental is complex)
microbes of different species are attached to tooth surface, mucous membrane of intestine and other body areas
biofilms - medical device….(eyes, heart, joints, cath, ute)
implantation ( catheters, heart valves, contact lenses, IUD, prosthetic joints etc)
biofilms - reservoir of..(strep for me was..)
pathogens for recurring infections - are formed on surfaces that are in contact with fluids
ex of cooperative interactions (cooperative mouth)
O2 utilization and anaerobic bacterial growth in mouth. biofilm allows anaerobic bacteria to survive
concentrate and conserve digestive enzymes are often…(conserve the matrix)
protected from immune attack due to the matrix; challenge for medical treatment
quarum sensing are (tart)
are attractive antimicrobial tarters.
quarum sensing gram + (circle pep)
small linear/circular peptides.
quarum sensing gram - (negative quarrel - joseph)
gram - acyl homoserine lactone.
generation time
generation time (different topic) - the time it takes for the bacterial pop. to double in size.
short generation time
e. coli has a short generation time of 20 min (if
nutrients are available) c. perfringans - 11 -12 min.
long generation time
Tuberculosis - 36-48 hour (very slow generation time - slow bc mycolic acid synthesis is a slow process)
consequence of slow generation time
anti-tb treatment are very prolonged - month to a year. protein turnover is very slow
biofilms in industry
microbes accumulation in pipes, drains and cooling towers
biofilms in nature
slipperiness of rocks
