HEMOGLOBINOPATHIES Flashcards
the hemoglobin is a tetramer of how many polypeptide chains?
4 polypeptides chains
the type of Hb is determined by the _____? how is this represented?
combination of the 4 globing chains
oxygen-hemoglobin dissociation curve
higher O2 affinity
higher pH
left or right shift?
left shift
lower O2 affinity
lower pH
left or right shift?
right shift
what are the 4 types of global chains?
alpha
beta
delta
gamma
this hemoglobin is the main O2 carrier in newborns?
T/F, higher O2 affinity than HbA and decreases during the first 6 months after birth
HbF
the main O2 carrier in adults
HbA
insignificant role in O2 transport
HbA2
levels of these hemoglobins indicate presence of a hemoglobinopathy?
HbF
HbA2
what are the methodologies used to detect hemoglobin subtypes?
hemoglobin electrophoresis
high performance liquid chromatography
this methodology can distinguish hemoglobin types present in a patient
hemoglobin electrophoresis
this methodology:
Hb through a column
charged solvent to move proteins
instrument detected quantities each Hb and produces a graphical read out
high performance liquid chromatography (HPLC)
what are the two main classifications of hemoglobinopathies?
qualitative
quantitative
this hemoglobinopathy is a globin gene mutation resulting in structural abnormalities
qualitative
this hemoglobinopathy is a globin gene mutation that results in decreased production of hemoglobin (thalassemias)
quantitative
