Growth and isolation Flashcards
All fungi require several specific elements for growth and reproduction.
T/F
T
The requirements for growth of fungi are generally less stringent than for _____________
sporulation
Constituents of media/agar
Generally contain a source of _____,_______, and _______
carbon, nitrogen and vitamins.
Constituents of media/agar
_________(_____) is the most widely utilizable carbon source, and hence is the most commonly used in growth media.
_______ and ________ are the next most commonly utilized sugars by fungi
Glucose (dextrose)
Fructose and mannose
Sucrose (table sugar) may be used in some media.
T/F
T
Constituents of media/agar
Nitrogen sources include ___,____ extract, ____ extract, _____, ammonium and nitrate compounds
peptone
yeast
Malt
Amino acid
Constituents of media/agar
Salts, including Fe, Zn and Mn, are often added to ‘ _____ ’ media, but are usually not added to the _____ media used for ________
defined
common
routine culture.
Fungi have natural deficiencies for vitamins that are satisfied at μM to nM concentrations.
T/F
T
Most common agar/media for growing fungi in the laboratory is _______
Most times incorporated in it is antibiotics (gentamycin, chloramphenicol) to inhibit bacterial growth. Exception is when being used for __________.
Saboraud Dextrose Agar (SDA).
cryptococcus
Is an Agar
Water Agar?
oat agar?
Malt extract agar?
Yes
Yes
Yes
Microscopy
Not necessary for identification of fungi
T/F
F
It is
Microscopy
Aids partially in determining the kind of media to use.
T/F
T
Microscopy Can not be used in presumptive diagnosis
F
It can
Microscopy Can be used in presumptive diagnosis e.g cryptococcus (________ stain)
india ink
Some fungi grow on routinely used agar such as blood agar, brain heart infusion etc.
T/F
T
