Gram Positive Cocci

Question 1

Staphylococci - a u r e u s

• (culture)Growth on ___ agar:

-• ______ colonies (_______)

Answer 1

Blood

yellow; goldenstaph

Question 2

Staphylococci - a u r e u s

•- (narrow or wide?) zone of beta hemolysis

• -Consistency of colonies is like _____, (can or can not?) be picked up easily by bacterial loop.

Answer 2

Narrow

butter

Can

Question 3

Streptococci pyogenes

• (culture) Growth on _____ agar

• -__________ size colonies

Answer 3

Blood

Pin point

Question 4

Streptococci pyogenes

•-(narrow or wide?) zone of betahemolysis

•-Consistency of colonies is ______, (can or can not?) be picked up easily by
bacterial loop.

Answer 4

Wide

tough

Can not

Question 5

Catalase Test

______coccus is positive (+) for this test

______coccus and ______coccus are negative (-).

Answer 5

Staphylo

Strepto

Pneumo

Question 6

Three important Staphylococcus species:

Staphylococcus ______

Staphylococcus _______

Staphylococcus ______

Answer 6

aureus

epidermidis

saprophyticus

Question 7

Which Staphylococcus species is a major pathogenic specie

Answer 7

Staphylococcus aureus

Question 8

Location and function of Staphylococcus epidermidis and Staphylococcus saprophyticus

Answer 8

Commensal on skin

Question 9

Differentiating features of S.aureus from other Staphylococci.

Coagulase test-(Positive or negative?)

______ on blood agar.

Answer 9

Positive

Hemolysis

Question 10

Differentiating features of S.aureus from other Staphylococci.

Ferment _______.

Grows in the presence 6.5% _____.

Answer 10

Mannitol

NaCl

Question 11

__________ Agar is a selective medium for S.aureus

Answer 11

Mannitol Salt

Question 12

________ test is used to differentiate between S.epidermidis and S.saprophyticus

Answer 12

Novobiocin

Question 13

differentiate between S.epidermidis and S.saprophyticus based on :

Novobiocin sensitivity

Habitat

Answer 13

Sensitive; resistant

Normal Human Skin; Skin & Paraurethral area

Question 14

Streptococcus can be divided into three based on hemolysis

Mention them

Answer 14

Alpha hemolytic
Beta hemolytic
Gamma hemolytic

Question 15

Differentiate the following in terms of color, degree of hemolysis, and examples

Alpha hemolytic
Beta hemolytic
Gamma hemolytic

Answer 15

Green; clear; red

Partial, total, none

Viridans and pneumoniae; agalactiae and Pyogenes; Enterococcus

Question 16

Group A streptococcus (Streptococcus _________)

Group B streptococcus (Streptococcus
_________)

Answer 16

pyogenes

agalactiae

Question 17

Enterococci belongs to Group ____ Streptococci

Answer 17

D

Question 18

Beta hemolytic streptococcus can be in which groups of streptococcus

Answer 18

A

B

C

G

Question 19

streptococcus pyogenes: live in
the ____,____,_________

Answer 19

nose and mouth. pharyngitis

Question 20

streptococcus pyogenes: live in
the nose and mouth. pharyngitis - ‘ _________ ‘, abscesses, otitis media and
sinusitis, cellulitis, rashes, gangrene, rheumatic fever and
toxic shock.

Answer 20

strep throat

Question 21

Streptococcus __________ In pregnant women, cause premature labor & urinary
tract infections

Answer 21

agalactiae

Question 22

Streptococcus agalactiae in the baby cause _______,______,______, and _______

Answer 22

pneumonia, meningitis, shock and bacteraemia.

Question 23

Bacitracin sensitivity test

beta hemolytic Streptococci are ( sensitive or resistant?) , except _______

Answer 23

resistant

group A streptococci

Question 24

CAMP test = __________________

Answer 24

Christie, Atkins, and Munch-Petersen.

Question 25

Christie, Atkins, and Munch-Petersen. Which streptococcus is positive and which is negative?

Answer 25

Group A is negative Group B is positive

Question 26

Mannitol salt agar Staphylococcus _____ ferments mannitol turns _____ to ______ ( due to ____ production) Staphylococcus _______ is a non mannitol fermenter , hence no color change(_____ ).

Answer 26

aureus; pink to yellow; acid epidermidis; pink

Question 27

Group A Streptococcus : Group B Streptococcus In terms Of : Camp test, Bacitracin, Diseases

Answer 27

Negative; positive Sensitive, resistant Soft tissue infections and pharyngitis.; Neonatal meningitis. Neonatal septicaemia.

Question 28

Mention 2 species of Enterococcus

Answer 28

faecalis & faecium.

Question 29

Enterococcus ________ is susceptible to Penicillin (Ampicillin), while Enterococcus __________ is resistant

Answer 29

Faecalis faecium

Question 30

BILE-ESCULIN test for ENTEROCOCCUS. PRINCIPLE. Certain bacteria are able to _________ in the _____ ( __% ____ or ___% ____).

Answer 30

hydrolyse esculin presence of bile 4; bile salt 40; bile

Question 31

Many bacteria can not hydrolyses esculin T/F

Answer 31

F Many bacteria can hydrolyses esculin, but not in the presence of bile

Question 32

Bacteria that are bile-esculin positive are able to 1st of all, ______ in the presence of _______, then ____ the ____ in the medium to form ____ and a compound called ______.

Answer 32

grow; bile salt hydolyse; esculin Glucose; esculetin

Question 33

Bacteria that are bile-esculin positive form Glucose and a compound called esculetin. Esculetin reacts with the ______, from the _____ in the medium to form ____ diffusible complex.

Answer 33

Ferric ion ; Ferric citrate black

Question 34

bile-esculin positive bacteria Example? _________

Answer 34

Enterococcus