Gram Positive Cocci Flashcards
Staphylococci - a u r e u s
• (culture)Growth on ___ agar:
-• ______ colonies (_______)
Blood
yellow; goldenstaph
Staphylococci - a u r e u s
•- (narrow or wide?) zone of beta hemolysis
• -Consistency of colonies is like _____, (can or can not?) be picked up easily by bacterial loop.
Narrow
butter
Can
Streptococci pyogenes
• (culture) Growth on _____ agar
• -__________ size colonies
Blood
Pin point
Streptococci pyogenes
•-(narrow or wide?) zone of betahemolysis
•-Consistency of colonies is ______, (can or can not?) be picked up easily by
bacterial loop.
Wide
tough
Can not
Catalase Test
______coccus is positive (+) for this test
______coccus and ______coccus are negative (-).
Staphylo
Strepto
Pneumo
Three important Staphylococcus species:
Staphylococcus ______
Staphylococcus _______
Staphylococcus ______
aureus
epidermidis
saprophyticus
Which Staphylococcus species is a major pathogenic specie
Staphylococcus aureus
Location and function of Staphylococcus epidermidis and Staphylococcus saprophyticus
Commensal on skin
Differentiating features of S.aureus from other Staphylococci.
Coagulase test-(Positive or negative?)
______ on blood agar.
Positive
Hemolysis
Differentiating features of S.aureus from other Staphylococci.
Ferment _______.
Grows in the presence 6.5% _____.
Mannitol
NaCl
__________ Agar is a selective medium for S.aureus
Mannitol Salt
________ test is used to differentiate between S.epidermidis and S.saprophyticus
Novobiocin
differentiate between S.epidermidis and S.saprophyticus based on :
Novobiocin sensitivity
Habitat
Sensitive; resistant
Normal Human Skin; Skin & Paraurethral area
Streptococcus can be divided into three based on hemolysis
Mention them
Alpha hemolytic
Beta hemolytic
Gamma hemolytic
Differentiate the following in terms of color, degree of hemolysis, and examples
Alpha hemolytic
Beta hemolytic
Gamma hemolytic
Green; clear; red
Partial, total, none
Viridans and pneumoniae; agalactiae and Pyogenes; Enterococcus
Group A streptococcus (Streptococcus _________)
Group B streptococcus (Streptococcus
_________)
pyogenes
agalactiae
Enterococci belongs to Group ____ Streptococci
D
Beta hemolytic streptococcus can be in which groups of streptococcus
A
B
C
G
streptococcus pyogenes: live in
the ____,____,_________
nose and mouth. pharyngitis
streptococcus pyogenes: live in
the nose and mouth. pharyngitis - ‘ _________ ‘, abscesses, otitis media and
sinusitis, cellulitis, rashes, gangrene, rheumatic fever and
toxic shock.
strep throat
Streptococcus __________ In pregnant women, cause premature labor & urinary
tract infections
agalactiae
Streptococcus agalactiae in the baby cause _______,______,______, and _______
pneumonia, meningitis, shock and bacteraemia.
Bacitracin sensitivity test
beta hemolytic Streptococci are ( sensitive or resistant?) , except _______
resistant
group A streptococci
CAMP test = __________________
Christie, Atkins, and Munch-Petersen.
Christie, Atkins, and Munch-Petersen.
Which streptococcus is positive and which is negative?
Group A is negative
Group B is positive
Mannitol salt agar
Staphylococcus _____ ferments mannitol turns _____ to ______ ( due to ____ production)
Staphylococcus _______ is a non mannitol fermenter , hence no color change(_____ ).
aureus; pink to yellow; acid
epidermidis; pink
Group A Streptococcus : Group B Streptococcus
In terms Of :
Camp test, Bacitracin, Diseases
Negative; positive
Sensitive, resistant
Soft tissue infections and pharyngitis.; Neonatal meningitis. Neonatal septicaemia.
Mention 2 species of Enterococcus
faecalis & faecium.
Enterococcus ________ is susceptible to Penicillin (Ampicillin), while Enterococcus __________ is resistant
Faecalis
faecium
BILE-ESCULIN test for ENTEROCOCCUS.
PRINCIPLE.
Certain bacteria are able to _________ in the _____ ( __% ____ or ___% ____).
hydrolyse esculin
presence of bile
4; bile salt
40; bile
Many bacteria can not hydrolyses esculin
T/F
F
Many bacteria can hydrolyses esculin, but not in the presence of bile
Bacteria that are bile-esculin positive are able to 1st of all, ______ in the presence of _______, then ____ the ____ in the medium to form ____ and a compound called ______.
grow; bile salt
hydolyse; esculin
Glucose; esculetin
Bacteria that are bile-esculin positive form Glucose and a compound called esculetin.
Esculetin reacts with the ______, from the _____ in the medium to form ____ diffusible complex.
Ferric ion ; Ferric citrate
black
bile-esculin positive bacteria
Example?
_________
Enterococcus
