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House Microbiology > Gram Positive Cocci > Flashcards

Gram Positive Cocci Flashcards

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Biology 101 flashcards
1
Q

Staphylococci - a u r e u s

• (culture)Growth on ___ agar:

-• ______ colonies (_______)

A

Blood

yellow; goldenstaph

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2
Q

Staphylococci - a u r e u s

•- (narrow or wide?) zone of beta hemolysis

• -Consistency of colonies is like _____, (can or can not?) be picked up easily by bacterial loop.

A

Narrow

butter

Can

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3
Q

Streptococci pyogenes

• (culture) Growth on _____ agar

• -__________ size colonies

A

Blood

Pin point

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4
Q

Streptococci pyogenes

•-(narrow or wide?) zone of betahemolysis

•-Consistency of colonies is ______, (can or can not?) be picked up easily by
bacterial loop.

A

Wide

tough

Can not

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5
Q

Catalase Test

______coccus is positive (+) for this test

______coccus and ______coccus are negative (-).

A

Staphylo

Strepto

Pneumo

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6
Q

Three important Staphylococcus species:

Staphylococcus ______

Staphylococcus _______

Staphylococcus ______

A

aureus

epidermidis

saprophyticus

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7
Q

Which Staphylococcus species is a major pathogenic specie

A

Staphylococcus aureus

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8
Q

Location and function of Staphylococcus epidermidis and Staphylococcus saprophyticus

A

Commensal on skin

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9
Q

Differentiating features of S.aureus from other Staphylococci.

Coagulase test-(Positive or negative?)

______ on blood agar.

A

Positive

Hemolysis

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10
Q

Differentiating features of S.aureus from other Staphylococci.

Ferment _______.

Grows in the presence 6.5% _____.

A

Mannitol

NaCl

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11
Q

__________ Agar is a selective medium for S.aureus

A

Mannitol Salt

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12
Q

________ test is used to differentiate between S.epidermidis and S.saprophyticus

A

Novobiocin

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13
Q

differentiate between S.epidermidis and S.saprophyticus based on :

Novobiocin sensitivity

Habitat

A

Sensitive; resistant

Normal Human Skin; Skin & Paraurethral area

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14
Q

Streptococcus can be divided into three based on hemolysis

Mention them

A

Alpha hemolytic
Beta hemolytic
Gamma hemolytic

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15
Q

Differentiate the following in terms of color, degree of hemolysis, and examples

Alpha hemolytic
Beta hemolytic
Gamma hemolytic

A

Green; clear; red

Partial, total, none

Viridans and pneumoniae; agalactiae and Pyogenes; Enterococcus

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16
Q

Group A streptococcus (Streptococcus _________)

Group B streptococcus (Streptococcus
_________)

A

pyogenes

agalactiae

17
Q

Enterococci belongs to Group ____ Streptococci

A

D

18
Q

Beta hemolytic streptococcus can be in which groups of streptococcus

A

A

B

C

G

19
Q

streptococcus pyogenes: live in
the ____,____,_________

A

nose and mouth. pharyngitis

20
Q

streptococcus pyogenes: live in
the nose and mouth. pharyngitis - ‘ _________ ‘, abscesses, otitis media and
sinusitis, cellulitis, rashes, gangrene, rheumatic fever and
toxic shock.

A

strep throat

21
Q

Streptococcus __________ In pregnant women, cause premature labor & urinary
tract infections

A

agalactiae

22
Q

Streptococcus agalactiae in the baby cause _______,______,______, and _______

A

pneumonia, meningitis, shock and bacteraemia.

23
Q

Bacitracin sensitivity test

beta hemolytic Streptococci are ( sensitive or resistant?) , except _______

A

resistant

group A streptococci

24
Q

CAMP test = __________________

A

Christie, Atkins, and Munch-Petersen.

25
Q

Christie, Atkins, and Munch-Petersen.

Which streptococcus is positive and which is negative?

A

Group A is negative
Group B is positive

26
Q

Mannitol salt agar

Staphylococcus _____ ferments mannitol turns _____ to ______ ( due to ____ production)

Staphylococcus _______ is a non mannitol fermenter , hence no color change(_____ ).

A

aureus; pink to yellow; acid

epidermidis; pink

27
Q

Group A Streptococcus : Group B Streptococcus

In terms Of :
Camp test, Bacitracin, Diseases

A

Negative; positive

Sensitive, resistant

Soft tissue infections and pharyngitis.; Neonatal meningitis. Neonatal septicaemia.

28
Q

Mention 2 species of Enterococcus

A

faecalis & faecium.

29
Q

Enterococcus ________ is susceptible to Penicillin (Ampicillin), while Enterococcus __________ is resistant

A

Faecalis

faecium

30
Q

BILE-ESCULIN test for ENTEROCOCCUS.

PRINCIPLE.

Certain bacteria are able to _________ in the _____ ( __% ____ or ___% ____).

A

hydrolyse esculin

presence of bile

4; bile salt

40; bile

31
Q

Many bacteria can not hydrolyses esculin

T/F

A

F

Many bacteria can hydrolyses esculin, but not in the presence of bile

32
Q

Bacteria that are bile-esculin positive are able to 1st of all, ______ in the presence of _______, then ____ the ____ in the medium to form ____ and a compound called ______.

A

grow; bile salt

hydolyse; esculin

Glucose; esculetin

33
Q

Bacteria that are bile-esculin positive form Glucose and a compound called esculetin.
Esculetin reacts with the ______, from the _____ in the medium to form ____ diffusible complex.

A

Ferric ion ; Ferric citrate

black

34
Q

bile-esculin positive bacteria

Example?

_________

A

Enterococcus

House Microbiology (93 decks)

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