Using a colorimeter to quantify glucose 1:

Question 1

Why is benedicts solution blue?

Answer 1

= contains cu2+ ions

Question 2

What can reducing sugars do?

Answer 2

= Donate an electron to another molecule

Question 3

If we leave the test tube for several of hours what happens?

Answer 3

= red precipitate would settle at the bottom
= allow to see the benedicts solution above it- less blue before the test
= some cu2+ iosn have reacted

Question 4

With the benedicts test the greater the concentration of glucose what happens?

Answer 4

= less blue the benedicts solution will be at the end
= may be too subtle to detect by eye

Question 5

How can we quantify the blueness of the solution?

Answer 5

= machine called colorimeter

Question 6

Before we use the colorimiter what do we do?

Answer 6

= need to filter out the red precipitate

Question 7

How does a colorimeter work?

Answer 7

= Shine a white light at a sample of benedicts solution
= all colors of white-light would be absorbed apart from blue- benedicts solution allows blue light to pass through it.

Question 8

What is the absorbtion of light used to quanify what?

Answer 8

= level of blueness

Question 9

What is an alternative color light that we can use that can be better?

Answer 9

= red
= benedicts solution absorbs red most out of the differrent colors
= opposiye end of the spectrum to blue

Question 10

What is the next steps that we do?

Answer 10

= place a red filter in front of lamp
= only allows red to pass through
= less absorbption and transmition–> less blue
=

Question 11

What is a red light detected by?

Answer 11

= photoelectric cell
= light detector