Microscopy: Flashcards

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1
Q

What is magnification?

A

= how many times bigger an image appears under the microscope

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2
Q

What is resolution?

A

= Smallest distance that 2 objects can be apart while appearing as 2 objects.

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3
Q

What is a light microscope?

A

= Uses light and leses to form a image of the specimen.

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4
Q

What are the advantages of a light microscope?

A

=Cheap
=Easy to use
= Study living cells

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5
Q

What are the disadvantages of a light microscope?

A

= Provides 2d imagery
= uses visible light to create images
= poor resolution- limited by the wavelength of light- longer wavelength
=

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6
Q

What is an electron microscope?

A

= Uses electrons and lenses to form an image of the specimen

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7
Q

What are the advantages of using a electron microscope?

A

= 3d imagery
= higher resolution- not limited by the wavelength of light
=

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8
Q

What are the disadvantages of using an electron microscope?

A

= has to be done in a vaccum, dead cell
= needs to be thin
= requires heavy staining

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9
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