Producing Monoclonal antibodies: Flashcards
1
Q
What is the first step?
A
= a mouse is injected with an antigen for the scientist are tryning to produce an antibody against
= b cells in the mouse produces specific antibodies extracted
2
Q
What is the second step?
A
= b-cells mixed with myeloma cells- divide rapidly
= b cells and myeloma cells fuse to form hybridomas
3
Q
Why are cancer cells used?
A
= divide readily outside the body
4
Q
What is the third step?
A
= hybridoma cells are seperated using light microscopy and each seperate cell is culture dto form clone of itself
= each culture made up of a single type of hybridoma cell that produce one type of monoclonal antibodies are tested to see if the desired is formed
