Testing For Biological Molecules: Reducing sugars., Flashcards

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1
Q

What are the 2 types of sugars?

A

= reducing and non-reducing sugars.

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2
Q

What is a reducing sugar?

A

= can donate an electron to another molecule.

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3
Q

Are monosacharides reducing or non-reducing and what about dissacharides?

A

ALL Monosacharrides are reducing
e.g: Glucose, fructose and Galactose.

SOME dissacharides.
e.g: Maltose + Lactose. (surcorse is non-reducing(,

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4
Q

What is a safety precaution for this practical?

A

= wearing goggles.

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5
Q

How do we start this practical?

A

= grinding the food with distilled water
= filtering away the solid food particles.
= place 3cm^3 of food solution into a boiling tube. `

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6
Q

What is the 2nd step for the practical?

A

= adding 3cm^3 of benedicts solution.
= place the boiling tube into a beaker of boiling water
= leave for 5 minutes.

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7
Q

What does benedicts solution contains?

A

= Cu2+ ion (copper ion)- makes solution blue.

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8
Q

If there is no reducing sugar present what happens?

A

= remains blue there is no reducing sugar present.

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9
Q

If reducing sugar is present what happens?

A

= Adds an electron to the CU+ ion.
= forms a Cu+ ion and red precipitate (solid).

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10
Q

If there is a small amount of reducing sugar present what happens?

A

= only a very small amount of reducing sugar forms a red precipitate.
= green.

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11
Q

If more reducing sugar is present what does the color turn into?

A

= color turns yellow.
= higher turns orange., `

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12
Q

What is the problem with using benedicts solution?

A

= gives us an approximate idea of the amount of reducing sugars.
= shows a narrow range of color changes
= all humans perceive colors differently.

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13
Q

Why do scientists call thebenedicts test semi-qunatitatve?

A

=

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