Testing For Biological Molecules: Reducing sugars., Flashcards
What are the 2 types of sugars?
= reducing and non-reducing sugars.
What is a reducing sugar?
= can donate an electron to another molecule.
Are monosacharides reducing or non-reducing and what about dissacharides?
ALL Monosacharrides are reducing
e.g: Glucose, fructose and Galactose.
SOME dissacharides.
e.g: Maltose + Lactose. (surcorse is non-reducing(,
What is a safety precaution for this practical?
= wearing goggles.
How do we start this practical?
= grinding the food with distilled water
= filtering away the solid food particles.
= place 3cm^3 of food solution into a boiling tube. `
What is the 2nd step for the practical?
= adding 3cm^3 of benedicts solution.
= place the boiling tube into a beaker of boiling water
= leave for 5 minutes.
What does benedicts solution contains?
= Cu2+ ion (copper ion)- makes solution blue.
If there is no reducing sugar present what happens?
= remains blue there is no reducing sugar present.
If reducing sugar is present what happens?
= Adds an electron to the CU+ ion.
= forms a Cu+ ion and red precipitate (solid).
If there is a small amount of reducing sugar present what happens?
= only a very small amount of reducing sugar forms a red precipitate.
= green.
If more reducing sugar is present what does the color turn into?
= color turns yellow.
= higher turns orange., `
What is the problem with using benedicts solution?
= gives us an approximate idea of the amount of reducing sugars.
= shows a narrow range of color changes
= all humans perceive colors differently.
Why do scientists call thebenedicts test semi-qunatitatve?
=