Enzymes: Effect of temperature on enzyme controlled reactions

Question 1

How can we investigate this?

Answer 1

= select an enzyme
= measure the amount of product formed in time
= repeat using a range of temperatures
= draw tangents to measure the rate of reaction at each temperatire

Question 2

As we increase the temperature what happens to the rate of reaction?

Answer 2

= increase temperature= increase rate of reaction- increasing the kinetic energy of the substrate and enzyme
= imcreases the chance for substrate collisions

Question 3

What happens eventually when we increase the temperature?

Answer 3

= at optimum temperature the rate of reaction is at the maximum- maximum frequency of collisions between substrate and active site

Question 4

What is the optimum temperature for human enzymes?

Answer 4

= 40 degrees celcius

Question 5

What happens if the temperature increases past the optimum temperature?

Answer 5

= rate of reaction rapildy decreases
= at high temperatures the enzyme molecule is vibrating more rapidly causes bonds within the enzymes to break
= teritary structure of the enzyme changes so active site is no longer complementary to the substrate
= DENATURED